Identification of suitable culture condition for expansion and osteogenic differentiation of human bone marrow stem cells

Human Cell

Volumn 25, Issue 3, 2012, Pages 69-77

  Chowdhury, Shiplu Roy ^a   Ng, Min Hwei ^a   Hassan, Norazril Shamsul Abu ^a,b   Aminuddin, Bin Saim ^a,c   Ruszymah, Bt Hj Idrus ^a,b  

^a UNIVERSITI KEBANGSAAN MALAYSIA MEDICAL CENTER   (Malaysia)

^b DEPARTMENT OF PHARMACOLOGY   (Malaysia)

^c Ampang Puteri Specialist Hospital Malaysia   (Malaysia)

Author keywords

Early osteogenic induction; Growth factors supplementation; In vitro expansion; Mesenchymal stem cells; Osteogenic differentiation; Tissue engineering

Indexed keywords

TRANSCRIPTION FACTOR RUNX2;

ALPHA MINIMUM ESSENTIAL MEDIUM; ARTICLE; BONE DEVELOPMENT; BONE MARROW CELL; BONE TISSUE; CASE REPORT; CELL DIFFERENTIATION; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; GENE EXPRESSION; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STEM CELL EXPANSION; TISSUE ENGINEERING;

EID: 84867131629     PISSN: 09147470     EISSN: 17490774     Source Type: Journal    
DOI: 10.1007/s13577-012-0045-4     Document Type: Article

References (33)

1. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284: 143-7.
2. Wakitani S, Goto T, Pineda SJ, et al. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am. 1994; 76: 579-92.
3. Tan KK, Aminuddin BS, Tan GH, et al. Strategy for generating tissue-engineered human bone construct. Med J Malaysia. 2004; 59(Suppl B): 43-4.
4. Oreffo RO, Cooper C, Mason C, Clements M. Mesenchymal stem cells: lineage, plasticity, and skeletal therapeutic potential. Stem Cell Rev. 2005; 1: 169-78.
5. Awad HA, Boivin GP, Dressler MR, Smith FN, Young RG, Butler DL. Repair of patellar tendon injuries using a cell-collagen composite. J Orthop Res. 2003; 21: 420-31.
6. Murphy JM, Fink DJ, Hunziker EB, Barry FP. Stem cell therapy in a caprine model of osteoarthritis. Arthritis Rheum. 2003; 48: 3464-74.
7. Ng MH, Aminuddin BS, Tan KK, Tan GH, Sabarul AM, Ruszymah BHI. The use of bone marrow stem cells for bone tissue engineering. Med J Malaysia. 2004; 59(Suppl B): 41-2.
8. Mauney JR, Volloch V, Kaplan DL. Role of adult mesenchymal stem cells in bone tissue engineering applications: current status and future prospects. Tissue Eng. 2005; 11: 787-802.
9. Yoshioka T, Mishima H, Ohyabu Y, et al. Repair of large osteochondral defects with allogeneic cartilaginous aggregates formed from bone marrow derived cells using RWV bioreactor. J Orthop Res. 2007; 25: 1291-8.
10. Sotiropoulou PA, Perez SA, Salagianni M, Baxevanis CN, Papamichail M. Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells. Stem Cells. 2006; 24: 462-71.
11. Shahdadfar A, Frønsdal K, Haug T, Reinholt FP, Brinchmann JE. In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells. 2005; 23: 1357-66.
12. van den Bos C, Mosca JD, Winkles J, Kerrigan L, Burgess WH, Marshak DR. Human mesenchymal stem cells respond to fibroblast growth factors. Hum Cell. 1997; 10: 45-50.
13. Louis MS, Michael K, Malissa M, Derek L. Insulin-like growth factor-i stimulates both cell growth and lipogenesis during differentiation of human mesenchymal stem cells into adipocytes. J Clin Endocrinol Metab. 2004; 89: 3543-53.
14. Kulterer B, Friedl G, Jandrositz A, et al. Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation. BMC Genomics. 2007; 8: 70-84.
15. Coelho MJ, Fernandes MH. Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, beta-glycerophosphate and dexamethasone on osteoblastic differentiation. Biomaterial. 2000; 21: 1095-102.
16. Malaval L, Modrowski D, Gupta AK, Aubin JE. Cellular expression of bone related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures. J Cell Physiol. 1994; 158: 555-72.
17. Jaiswal N, Haynesworth SE, Caplan AL, Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem. 1997; 64: 295-312.
18. Aubin JE, Turksen K, Heersche JNM. Osteoblastic cell lineage: cellular and molecular biology of bone. In: Noda M, editor. New York: Academic; 1993. p. 1-45.
19. Aubin JE. Advances in the osteoblast lineage. Biochem Cell Biol. 1998; 76: 899-910.
20. Tan KK, Tan GH, Shamsul BS, et al. Bone graft substitute using hydroxyapatite scaffold seeded with tissue engineered autologous osteoprogenitor cells in spinal fusion: early result in a sheep model. Med J Malaysia. 2005; 60(Suppl C): 53-8.
21. Sharen SharenAini S, Masfueh R, Badiah B, et al. Challenges in culturing Macaca fascicularis bone marrow stem cells. Sains Malaysiana. 2012; 41: 419-22.
22. Ng MH, Aminuddin BS, Tan KK, et al. Comparison of bioengineered human bone construct from four sources of osteogenic cells. J Ortho Sci. 2005; 10: 192-9.
23. Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV. Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology. 1994; 134: 277-86.
24. Choi KM, Seo YK, Yoon HH, et al. Effect of ascorbic acid on bone marrow-derived mesenchymal stem cell proliferation and differentiation. J Biosci Bioeng. 2008; 105: 586-94.
25. Xiao Y, Peperzak V, van Rijn L, Borst J, de Bruijn J. Dexamethasone treatment during the expansion phase maintains stemness of bone marrow mesenchymal stem cells. J Tissue Eng Regen Med. 2010; 4: 374-86.
26. Hamoud HA, Chua KH, Aminuddin BS, Ruszymah BHI. Growth medium with low serum and transforming growth factor beta 3 promotes better chondrogenesis of bone marrow-derived stem cells in vitro and in vivo. Saudi Med J. 2011; 32: 640-1.
27. Tsutsumi S, Shimazu A, Miyazaki K, et al. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun. 2001; 288: 413-9.
28. Benavente CA, Sierralta WD, Conget PA, Minguell JJ. Subcellular distribution and mitogenic effect of basic fibroblast growth factor in mesenchymal uncommitted stem cells. Growth Factors. 2003; 21: 87-94.
29. Ng F, Boucher S, Koh S, et al. PDGF, TGF-β, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages. Blood. 2008; 112: 295-307.
30. Li WG, Xu XX. The expression of N-cadherin, fibronectin during chondrogenic differentiation of MSC induced by TGF-beta1. Chin J Traumatol. 2005; 8: 349-51.
31. Jeong WK, Park SW, Im GI. Growth factors reduce the suppression of proliferation and osteogenic differentiation by titanium particles on MSCs. J Biomed Mater Res A. 2008; 86A: 1137-44.
32. Fakhry A, Ratisoontorn C, Vedhachalam C, et al. Effects of FGF-2/-9 in calvarial bone cell cultures: differentiation stage-dependent mitogenic effect, inverse regulation of BMP-2 and noggin, and enhancement of osteogenic potential. Bone. 2005; 36: 254-66.
33. Hatch NE, Nociti F, Swanson E, Bothwell M, Somerman M. FGF2 alters expression of the pyrophosphate/phosphate regulating proteins, PC-1, ANK and TNAP, in the calvarial osteoblastic cell line, MC3T3E1(C4). Connect Tissue Res. 2005; 46: 184-92.