Cell-engineered human elastic chondrocytes regenerate natural scaffold in vitro and neocartilage with neoperichondrium in the human body post-transplantation

Tissue Engineering - Part A

Volumn 18, Issue 19-20, 2012, Pages 2020-2029

  Yanaga, Hiroko ^a   Imai, Keisuke ^b   Koga, Mika ^a   Yanaga, Katsu ^a  

^a Yanaga Clinic and Tissue Culture Laboratory   (Japan)

^b OSAKA CITY GENERAL HOSPITAL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; CARTILAGE; CELL CULTURE; CELL ENGINEERING; CELLS; ORGANIC ACIDS; TISSUE;

BIOCHEMICAL CHANGES; CELL COUNT; CHONDROCYTES; CLINICAL APPLICATION; CULTURE MEDIUM; CULTURE SYSTEMS; EXTRACELLULAR MATRICES; FIBROBLAST GROWTH FACTOR-2; HUMAN BODIES; HYALURONIC ACIDS; IMMUNOHISTOCHEMICAL ANALYSIS; IN-SITU; IN-VITRO; LIVING BODIES; MATRIX FORMATION; MICROSCOPIC ANALYSIS; MULTI-LAYERED; NEOCARTILAGE; THREE-DIMENSIONAL STRUCTURE; TISSUE INTEGRITY;

SCAFFOLDS (BIOLOGY);

FIBROBLAST GROWTH FACTOR 2; HYALURONIC ACID;

ARTICLE; CARTILAGE CELL; CELL CULTURE; CYTOLOGY; DRUG EFFECT; EAR CARTILAGE; ELASTIC CARTILAGE; HUMAN; METABOLISM; METHODOLOGY; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING;

CELLS, CULTURED; CHONDROCYTES; EAR CARTILAGE; ELASTIC CARTILAGE; FIBROBLAST GROWTH FACTOR 2; HUMANS; HYALURONIC ACID; MICROSCOPY, ELECTRON, SCANNING; TISSUE ENGINEERING;

EID: 84867021110     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2011.0370     Document Type: Article

References (39)

1. Yanaga, H., Koga, M., Imai, K., and Yanaga, K. Clinical application of biotechnically cultured autologous chon-drocytes as novel graft material for nasal augmentation. Aesthetic Plast Surg 28, 212, 2004.
2. Yanaga, H., Yanaga, K., Imai, K., Koga, M., Soejima, C., and Ohmori, K. Clinical application of cultured autologous human auricular chondrocytes with autologous serum for craniofacial or nasal augmentation and repair. Plast Reconstr Surg 117, 2019, 2006.
3. Yanaga, H., Imai, K., and Yanaga, K. Generative surgery of cultured autologous auricular chondrocytes for nasal augmentation. Aesthetic Plast Surg 33, 795, 2009.
4. Yanaga, H., Imai, K., Fujimoto, T., and Yanaga, K. Generating ears from cultured autologous auricular chon-drocytes by using two-stage implantation in treatment of microtia. Plast Reconstr Surg 124, 817, 2009.
5. Vacanti, C.A., Langer, R., Schloo, B., and Vacanti, J.P. Synthetic polymers seeded with chondrocytes provide a template for new cartilage formation. Plast Reconstr Surg 88, 753, 1991.
6. Cao, Y., Vacanti, J.P., Paige, K.T., Upton, J., and Vacanti, C.A. Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear. Plast Reconstr Surg 100, 297, 1997.
7. Rodriguez, A., Cao, Y.L., Ibarra, C., Pap, S., Vacanti, M., Eavey, R.D., et al. Characteristics of cartilage engineered from human pediatric auricular cartilage. Plast Reconstr Surg 103, 1111, 1999.
8. Ting, V., Sims, C.D., Brecht, L.E., McCarthy, J.G., Kasabian, A.K., Connelly, P.R., et al. In vitro prefabrication of human cartilage shapes using fibrin glue and human chondrocytes. Ann Plast Surg 40, 413, 1998.
9. van Osch, G.J., van der Veen, S.W., and Verwoerd-Verhoef, H.L. In vitro redifferentiation of culture-expanded rabbit and human auricular chondrocytes for cartilage reconstruction. Plast Reconstr Surg 107, 433, 2001.
10. Tay, A.G., Farhadi, J., Suetterlin, R., Pierer, G., Heberer, M., and Martin, I. Cell yield, proliferation, and postexpansion differentiation capacity of human ear, nasal, and rib chon-drocytes. Tissue Eng 10, 762, 2004.
11. Kamil, S.H., Kojima, K., Vacanti, M.P., Bonassar, L.J., Vacanti, C.A., and Eavey, R.D. In vitro tissue engineering to generate a human-sized auricle and nasal tip. Laryngoscope 113, 90, 2003.
12. Chang, S.C., Tobias, G., Roy, A.K., Vacanti, C.A., and Bo-nassar, L.J. Tissue engineering of autologous cartilage for craniofacial reconstruction by injection molding. Plast Re-constr Surg 112, 793, 2003.
13. von der Mark, K., Gauss, V., von der Mark, H., and Müller, P. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 267, 531, 1977.
14. Schiltz, J.R., Mayne, R., and Holtzer, H. The synthesis of collagen and glycosaminoglycans by dedifferentiated chon-droblasts in culture. Differentiation 1, 97, 1977.
15. Aulthouse, A.L., Beck, M., Griffey, E., Sanford, J., Arden, K., and Machado, M.A., et al. Expression of the human chon-drocyte phenotype in vitro. In Vitro Cell Dev Biol 25, 659, 1989.
16. Binderman, I., and Sömjen, D. 24, 25-Dihydroxy-cholecalciferol induces the growth of chick cartilage in vitro. Endocrinology 115, 430, 1984.
17. Giovannini, S., Diaz-Romero, J., Aigner, T., Heini, P., Mainil-Varlet, P., and Nesic, D. Micromass co-culture of human articular chondrocytes and human bone marrow mesen-chymal stem cells to investigate stable neocatilage tissue formation in vitro. Eur Cells Mater 20, 245, 2010.
18. Roy, R., Kudryashov, V., Doty, S.B., Binderman, I., and Boskey, A.L Differentiation and mineralization of murine mesenchymal C3H10T1/2 cells in micromass culture. Differentiation 79, 211, 2010.
19. Arévalo-Silva, C.A., Cao, Y., Weng, Y., Vacanti, M., Ro-dríguez, A., Vacanti, C.A., et al. The effect of fibroblast growth factor and transforming growth factor-beta on porcine chondrocytes and tissue-engineered autologous elastic cartilage. Tissue Eng 7, 81, 2001.
20. Weksler, N.B., Lunstrum, G.P., Reid, E.S., and Horton, W.A. Differential effects of fibroblast growth factor (FGF) 9 and FGF2 on proliferation, differentiation and terminal differentiation of chondrocytic cells in vitro. Biochem J 342, 677, 1999.
21. Jakob, M., Démarteau, O., Schäfer, D., Hintermann, B., Dick, W., Heberer, M., et al. Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro. J Cell Biochem 81, 368, 2001.
22. Praul, C.A., Ford, B.C., and Leach, R.M. Effect of fibroblast growth factors 1, 2, 4, 5, 6, 7, 8, 9, and 10 on avian chon-drocyte proliferation. J cell Biochem 84, 359, 2002.
23. Mandl, E.W., van der Veen, S.W., Verhaar, J.A., and van Osch, G.J. Serum-Free medium supplemented with high-concentration FGF2 for cell expansion culture of human ear chondrocytes promotes redifferentiation capacity. Tissue Eng 8, 573, 2002.
24. Mandl, E.W., Jahr, H., Koevoet, J.L., van Leeuwen, J.P., Weinans, H., Verhaar, J.A., et al. Fibroblast growth factor-2 in serum-free medium is a potent mitogen and reduces dedifferentiation of human ear chondrocytes in monolayer culture. Matrix Biol 23, 231, 2004.
25. Engström-Laurent, A., Lööf. L., Nyberg, A., and Schrö der, T. Increased serum levels of hyaluronate in liver disease. He-patology 5, 638, 1985.
26. Meanrd, K.P. Dynamic Mechanical Analysis: A Practical Introduction. New York: CRC Press, 2008. pp. 1-15.
27. Ma, Z., Gao, C., Gong, Y., and Shen, J. Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor. Biomaterials 26, 1253, 2005.
28. Freed, L.E., Marquis, J.C., Nohria, A., Emmanual, J., Mikos, A.G., and Langer, R. Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 27, 11, 1993.
29. Lu, L., Stamatas, G.N., and Mikos, A.G. Controlled release of transforming growth factor beta1 from biodegradable polymer microparticles. J Biomed Mater Res 5, 440, 2000.
30. Liu, H., Lee, Y.W., and Dean, M.F. Re-expression of differentiated proteoglycan phenotype by dedifferentiated human chondrocytes during culture in alginate beads. Biochimi Biophys Acta 1425, 505, 1998.
31. Benya, P.D., and Shaffer, J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30, 215, 1982.
32. Chang, C.H., Liu, H.C., Lin, C.C., Chou, C.H., and Lin, F.H. Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering. Biomaterials 24, 4853, 2003.
33. Ma, L., Gao, C., Mao, Z., Zhou, J., and Shen, J. Enhanced biological stability of collagen porous scaffolds by using amino acids as novel cross-linking bridges. Biomaterials 25, 2997, 2004.
34. van Susante, J.L.C., Pieper, J., Buma, P., van Kuppevelt, T.H., van Beuningen, H., van Der Kraan, P.M., et al. Linkage of chondroitin-sulfate to type I collagen scaffolds stimulates the bioactivity of seeded chondrocytes in vitro. Biomaterials 22, 2359, 2001.
35. Xia, W., Liu, W., Cui, L., Liu, Y., Zhong, W., Liu, D., et al. Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds. J Biomed Mater Res B Appl Biomater 71, 373-80, 2004. (Pubitemid 39463774)
36. Suh, J.K., and Matthew, H.W. Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials 21, 2589, 2000.
37. Perka, C., Arnold, U., Spitzer, R.S., and Lindenhayn, K. The use of fibrin beads for tissue engineering and subsequential transplantation. Tissue Eng 7, 359, 2001.
38. Lisignoli, G., Cristino, S., Piacentini, A., Toneguzzi, S., Grassi, F., Cavallo, C., et al. Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold. Biomaterials 26, 5677, 2005.
39. Green, H. Therapy with Cultured Cells. Singapore: Pan Stanford Publishing Pte. Ltd. www.panstanford.com, 2010, pp. 55-56.