Secretome analysis of chondroitin sulfate-treated chondrocytes reveals anti-angiogenic, anti-inflammatory and anti-catabolic properties

Arthritis Research and Therapy

Volumn 14, Issue 5, 2012, Pages

  Calamia, Valentina ^a   Lourido, Lucía ^a   Fernández Puente, Patricia ^a   Mateos, Jesús ^a   Rocha, Beatriz ^a   Montell, Eulalia ^b   Vergés, Josep ^b   Ruiz Romero, Cristina ^a,c   Blanco, Francisco J ^a  

^a Osteoarticular and Aging Research Lab   (Spain)

^b I3 Research   (Spain)

^c INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTITRYPSIN; ALTERNATIVE COMPLEMENT PATHWAY C3 C5 CONVERTASE; BETA 2 MICROGLOBULIN; BIGLYCAN; CATHEPSIN B; CELL PROTEIN; CFAB PROTEIN; CHITINASE 3 LIKE PROTEIN 1; CHITINASE 3 LIKE PROTEIN 2; CHONDROITIN SULFATE; CLUSTERIN; CO3 PROTEIN; COILED COIL DOMAIN CONTAINING PROTEIN 80; COMPLEMENT; COMPLEMENT COMPONENT C1R; COMPLEMENT COMPONENT C1S; CXCL3 CHEMOKINE; EPITHELIAL DERIVED NEUTROPHIL ACTIVATING FACTOR 78; GELATINASE A; GRANULOCYTE CHEMOTACTIC PROTEIN 2; INTERLEUKIN 1BETA; INTERSTITIAL COLLAGENASE; ISOTOPE; LIPOCORTIN 2; MONOCYTE CHEMOTACTIC PROTEIN 1; MONOCYTE CHEMOTACTIC PROTEIN 2; STROMELYSIN; THROMBOSPONDIN 1; TSG6 PROTEIN; UNCLASSIFIED DRUG; UNINDEXED DRUG; ANABOLIC AGENT; ANGIOGENESIS INHIBITOR; ANTIINFLAMMATORY AGENT; CELL ADHESION MOLECULE; TNFAIP6 PROTEIN, HUMAN;

ANTIANGIOGENIC ACTIVITY; ANTIINFLAMMATORY ACTIVITY; ARTICLE; CARTILAGE CELL; CATABOLISM; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEOMICS; PSEUDOMONAS PUTIDA; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING; CELL CULTURE; COMPARATIVE STUDY; DRUG EFFECTS; METABOLISM;

ANABOLIC AGENTS; ANGIOGENESIS INHIBITORS; ANTI-INFLAMMATORY AGENTS; CELL ADHESION MOLECULES; CELLS, CULTURED; CHONDROCYTES; CHONDROITIN SULFATES; COMPLEMENT C1R; COMPLEMENT C1S; HUMANS; INTERLEUKIN-1BETA; MATRIX METALLOPROTEINASE 1; MATRIX METALLOPROTEINASE 3; THROMBOSPONDIN 1;

EID: 84866788862     PISSN: 14786354     EISSN: 14786362     Source Type: Journal    
DOI: 10.1186/ar4040     Document Type: Article

References (36)

1. Martel-Pelletier J, Lajeunesse D, Pelletier J. Etiopathogenesis of osteoarthritis. Arthritis & Allied Conditions. A Textbook of Rheumatology 2005, 2199-2226. Baltimore, MD: Lippincott, Williams & Wilkins, Koopman WJ, Moreland LW, 15, 3460249, 23060942.
2. Alcaraz MJ, Megías J, García-Arnandis I, Clérigues V, Guillén MI. New molecular targets for the treatment of osteoarthritis. Biochem Pharmacol 2010, 80:13-21. 10.1016/j.bcp.2010.02.017, 20206140.
3. Imada K, Oka H, Kawasaki D, Miura N, Sato T, Ito A. Anti-arthritic action mechanisms of natural chondroitin sulfate in human articular chondrocytes and synovial fibroblasts. Biol Pharm Bull 2010, 33:410-414. 10.1248/bpb.33.410, 20190401.
4. Monfort J, Pelletier JP, Garcia-Giralt N, Martel-Pelletier J. Biochemical basis of the effect of chondroitin sulphate on osteoarthritis articular tissues. Ann Rheum Dis 2008, 67:735-740. 10.1136/ard.2006.068882, 17644553.
5. Kahan A, Uebelhart D, De Vathaire F, Delmas PD, Reginster JY. Long-term effects of chondroitins 4 and 6 sulfate on knee osteoarthritis: the study on osteoarthritis progression prevention, a two-year, randomized, double-blind, placebo-controlled trial. Arthritis Rheum 2009, 60:524-533. 10.1002/art.24255, 19180484.
6. Sawitzke AD, Shi H, Finco MF, Dunlop DD, Harris CL, Singer NG, Bradley JD, Silver D, Jackson CG, Lane NE, Oddis CV, Wolfe F, Lisse J, Furst DE, Bingham CO, Reda DJ, Moskowitz RW, Williams HJ, Clegg DO. Clinical efficacy and safety of glucosamine, chondroitin sulphate, their combination, celecoxib or placebo taken to treat osteoarthritis of the knee: 2-year results from GAIT. Ann Rheum Dis 2010, 69:1459-1464. 10.1136/ard.2009.120469, 3086604, 20525840.
7. McAlindon TE, LaValley MP, Gulin JP, Felson DT. Glucosamine and chondroitin for treatment of osteoarthritis: a systematic quality assessment and meta-analysis. JAMA 2000, 283:1469-1475. 10.1001/jama.283.11.1469, 10732937.
8. Clegg DO, Reda DJ, Harris CL, Klein MA, O'Dell JR, Hooper MM, Bradley JD, Bingham CO, Weisman MH, Jackson CG, Lane NE, Cush JJ, Moreland LW, Schumacher HR, Oddis CV, Wolfe F, Molitor JA, Yocum DE, Schnitzer TJ, Furst DE, Sawitzke AD, Shi H, Brandt KD, Moskowitz RW, Williams HJ. Glucosamine, chondroitin sulfate, and the two in combination for painful knee osteoarthritis. N Engl J Med 2006, 354:795-808. 10.1056/NEJMoa052771, 16495392.
9. Calamia V, Ruiz-Romero C, Rocha B, Fernandez-Puente P, Mateos J, Montell E, Verges J, Blanco FJ. Pharmacoproteomic study of the effects of chondroitin and glucosamine sulfate on human articular chondrocytes. Arthritis Res Ther 2010, 12:R138. 10.1186/ar3077, 2945029, 20626852.
10. Fernandes JC, Martel-Pelletier J, Pelletier JP. The role of cytokines in osteoarthritis pathophysiology. Biorheology 2002, 39:237-246.
11. Piersma SR, Fiedler U, Span S, Lingnau A, Pham TV, Hoffmann S, Kubbutat MH, Jimenez CR. Workflow comparison for label-free, quantitative secretome proteomics for cancer biomarker discovery: method evaluation, differential analysis, and verification in serum. J Proteome Res 2010, 9:1913-1922. 10.1021/pr901072h, 20085282.
12. Calamia V, Rocha B, Mateos J, Fernández-Puente P, Ruiz-Romero C, Blanco FJ. Metabolic labeling of chondrocytes for the quantitative analysis of the interleukin-1-beta-mediated modulation of their intracellular and extracellular proteomes. J Proteome Res 2011, 10:3701-3711. 10.1021/pr200331k, 21692455.
13. Ruiz-Romero C, López-Armada MJ, Blanco FJ. Proteomic characterization of human normal articular chondrocytes: a novel tool for the study of osteoarthritis and other rheumatic diseases. Proteomics 2005, 5:3048-3059. 10.1002/pmic.200402106, 16035116.
14. Tat SK, Pelletier JP, Mineau F, Duval N, Martel-Pelletier J. Variable effects of 3 different chondroitin sulfate compounds on human osteoarthritic cartilage/chondrocytes: relevance of purity and production process. J Rheumatol 2010, 37:656-664. 10.3899/jrheum.090696, 20110528.
15. Roche Applied Science. , http://www.roche-applied-science.com
16. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25:402-408. 10.1006/meth.2001.1262, 11846609.
17. Dudhia J. Aggrecan, aging and assembly in articular cartilage. Cell Mol Life Sci 2005, 62:2241-2256. 10.1007/s00018-005-5217-x, 16143826.
18. Martel-Pelletier J, Kwan Tat S, Pelletier JP. Effects of chondroitin sulfate in the pathophysiology of the osteoarthritic joint: a narrative review. Osteoarthritis Cartilage 2010, 18:S7-S11.
19. Polacek M, Bruun JA, Johansen O, Martinez I. Differences in the secretome of cartilage explants and cultured chondrocytes unveiled by SILAC technology. J Orthop Res 2010, 28:1040-1049.
20. Clutterbuck AL, Smith JR, Allaway D, Harris P, Liddell S, Mobasheri A. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics 2011, 74:704-715. 10.1016/j.jprot.2011.02.017, 3078332, 21354348.
21. Kapoor M, Martel-Pelletier J, Lajeunesse D, Pelletier JP, Fahmi H. Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 2011, 7:33-42. 10.1038/nrrheum.2010.196, 21119608.
22. Fernández-Puente P, Mateos J, Fernández-Costa C, Oreiro N, Fernández-López C, Ruiz-Romero C, Blanco FJ. Identification of a panel of novel serum osteoarthritis biomarkers. J Proteome Res 2011, 10:5095-5101. 10.1021/pr200695p, 21973172.
23. Mateos J, Lourido L, Fernández-Puente P, Calamia V, Fernández-López C, Oreiro N, Ruiz-Romero C, Blanco FJ. Differential protein profiling of synovial fluid from rheumatoid arthritis and osteoarthritis patients using LC-MALDI TOF/TOF. J Proteomics 2012, 75:2869-2878. 10.1016/j.jprot.2011.12.042, 22245418.
24. Ong SE, Mann M. A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). Nat Protoc 2006, 1:2650-2660.
25. Zivanović S, Rackov LP, Vojvodić D, Vucetić D. Human cartilage glycoprotein 39 - biomarker of joint damage in knee osteoarthritis. Int Orthop 2009, 33:1165-1170. 10.1007/s00264-009-0747-8, 2898969, 19308408.
26. Homandberg GA, Wen C, Hui F. Cartilage damaging activities of fibronectin fragments derived from cartilage and synovial fluid. Osteoarthritis Cartilage 1998, 6:231-244. 10.1053/joca.1998.0116, 9876392.
27. Zack MD, Arner EC, Anglin CP, Alston JT, Malfait AM, Tortorella MD. Identification of fibronectin neoepitopes present in human osteoarthritic cartilage. Arthritis Rheum 2006, 54:2912-2922. 10.1002/art.22045, 16948117.
28. Calamia V, Fernandez-Puente P, Mateos J, Lourido L, Rocha B, Montell E, Verges J, Ruiz-Romero C, Blanco FJ. Pharmacoproteomic study of three different chondroitin sulfate compounds on intracellular and extracellular human chondrocyte proteomes. Mol Cell Proteomics 2012, 11:M111.013417. 10.1074/mcp.M111.013417, 22203690.
29. Wang Q, Rozelle AL, Lepus CM, Scanzello CR, Song JJ, Larsen DM, Crish JF, Bebek G, Ritter SY, Lindstrom TM, Hwang I, Wong HH, Punzi L, Encarnacion A, Shamloo M, Goodman SB, Wyss-Coray T, Goldring SR, Banda NK, Thurman JM, Gobezie R, Crow MK, Holers VM, Lee DM, Robinson WH. Identification of a central role for complement in osteoarthritis. Nat Med 2011, 17:1674-1679. 10.1038/nm.2543, 3257059, 22057346.
30. Wisniewski HG, Hua JC, Poppers DM, Naime D, Vilcek J, Cronstein BN. TNF/IL-1-inducible protein TSG-6 potentiates plasmin inhibition by inter-alpha-inhibitor and exerts a strong anti-inflammatory effect in vivo. J Immunol 1996, 156:1609-1615.
31. Hsieh JL, Shen PC, Shiau AL, Jou IM, Lee CH, Wang CR, Teo ML, Wu CL. Intraarticular gene transfer of thrombospondin-1 suppresses the disease progression of experimental osteoarthritis. J Orthop Res 2010, 28:1300-1306. 10.1002/jor.21134, 20309955.
32. Silvestre JS, Théry C, Hamard G, Boddaert J, Aguilar B, Delcayre A, Houbron C, Tamarat R, Blanc-Brude O, Heeneman S, Clergue M, Duriez M, Merval R, Lévy B, Tedgui A, Amigorena S, Mallat Z. Lactadherin promotes VEGF-dependent neovascularization. Nat Med 2005, 11:499-506. 10.1038/nm1233, 15834428.
33. Lambert C, Mathy-Hartert M, Dubuc JE, Montell E, Vergés J, Munaut C, Noël A, Henrotin Y. Characterization of synovial angiogenesis in osteoarthritis patients and its modulation by chondroitin sulfate. Arthritis Res Ther 2012, 14:R58. 10.1186/ar3771, 3446424, 22409996.
34. Miller RR, McDevitt CA. Thrombospondin is present in articular cartilage and is synthesized by articular chondrocytes. Biochem Biophys Res Commun 1988, 153:708-714. 10.1016/S0006-291X(88)81152-5, 3289537.
35. Bornstein P. Thrombospondins function as regulators of angiogenesis. J Cell Commun Signal 2009, 3:189-200. 10.1007/s12079-009-0060-8, 2778581,2778581, 19798599.
36. Haywood L, McWilliams DF, Pearson CI, Gill SE, Ganesan A, Wilson D, Walsh DA. Inflammation and angiogenesis in osteoarthritis. Arthritis Rheum 2003, 48:2173-2177. 10.1002/art.11094, 12905470.