Protein interaction affinity determination by quantitative FRET technology

Biotechnology and Bioengineering

Volumn 109, Issue 11, 2012, Pages 2875-2883

  Song, Yang ^a   Rodgers, V G J ^a   Schultz, Jerome S ^a   Liao, Jiayu ^a,b  

^a University of California Riverside   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fluorescent proteins; Kd determination; Quantitative FRET; SUMOylation

Indexed keywords

ACCEPTOR CONCENTRATIONS; ACCURACY TESTING; BIOLOGICAL STUDIES; CASCADE REACTIONS; DISSOCIATION CONSTANT; FLUORESCENT PROTEIN; HIGH SENSITIVITY; IN-VITRO; ISOTHERMAL TITRATION CALORIMETRY; MULTI-STEP; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN INTERACTION; PROTEIN-PROTEIN INTERACTIONS; QUANTITATIVE FRET; RESONANCE ENERGY TRANSFER; SUMOYLATION; TEST SYSTEMS;

DISSOCIATION; ENERGY TRANSFER;

PROTEINS;

SUMO 1 PROTEIN;

ACCURACY; ARTICLE; BINDING AFFINITY; BIOLOGICAL ACTIVITY; CONJUGATION; COVALENT BOND; DISSOCIATION CONSTANT; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; OPEN READING FRAME; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; SURFACE PLASMON RESONANCE;

FLUORESCENCE RESONANCE ENERGY TRANSFER; KINETICS; PROTEIN BINDING; PROTEINS; SUMO-1 PROTEIN; UBIQUITIN-CONJUGATING ENZYMES;

EID: 84866774623     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24564     Document Type: Article

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