Development of FRET assay into quantitative and high-throughput screening technology platforms for protein-protein interactions

Annals of Biomedical Engineering

Volumn 39, Issue 4, 2011, Pages 1224-1234

  Song, Yang ^a   Madahar, Vipul ^a   Liao, Jiayu ^a,b  

^a University of California at Riverside ^*  (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

F rster resonance energy transfer; High throughput screening; K d affinity determination; SUMOylation

Indexed keywords

AFFINITY MEASUREMENT; BIOMEDICAL RESEARCH; CASCADE REACTIONS; CELL-BASED; CELLULAR PROCESS; DISSOCIATION CONSTANT; EXPERIMENTAL PROCEDURE; FLUORESCENT ENERGY TRANSFERS; HIGH EFFICIENCY; HIGH-THROUGHPUT; HIGH-THROUGHPUT SCREENING; IN-CELL; ISOTHERMAL TITRATION CALORIMETRY; K D AFFINITY DETERMINATION; MULTI-STEP; PROTEIN INTERACTION; PROTEIN-PROTEIN INTERACTIONS; RESONANCE ENERGY TRANSFER; STEADY STATE; SUMOYLATION; UBIQUITINATION; WELL PLATES;

DISSOCIATION; ENERGY TRANSFER; SURFACE PLASMON RESONANCE; THROUGHPUT;

PROTEINS;

HYBRID PROTEIN; PHOTOPROTEIN; SUMO 1 PROTEIN; UBIQUITIN CONJUGATING ENZYME; UBIQUITIN CONJUGATING ENZYME UBC9; UBIQUITIN-CONJUGATING ENZYME UBC9;

ARTICLE; BIOENGINEERING; CELL STRAIN HEK293; EVALUATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; HUMAN; KINETICS; METABOLISM; METHODOLOGY; PROTEIN ANALYSIS; SUMOYLATION; SURFACE PLASMON RESONANCE;

BIOENGINEERING; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; KINETICS; LUMINESCENT PROTEINS; PROTEIN INTERACTION MAPPING; RECOMBINANT FUSION PROTEINS; SUMO-1 PROTEIN; SUMOYLATION; SURFACE PLASMON RESONANCE; UBIQUITIN-CONJUGATING ENZYMES;

EID: 79954414627     PISSN: 00906964     EISSN: 15739686     Source Type: Journal    
DOI: 10.1007/s10439-010-0225-x     Document Type: Article

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