Enzymolysis of high density lipoprotein with a combination of membrane-immobilized esterase and trypsin

Journal of Pharmaceutical and Biomedical Analysis

Volumn 71, Issue , 2012, Pages 179-182

  Sakikawa, Takahiro ^a   Shimazaki, Youji ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

6,9 Diamino 2 ethoxyacridine; Carboxylesterase; Electrophoresis; HDL; MALDI TOF MS; Trypsin

Indexed keywords

APOLIPOPROTEIN A1; ESTERASE; ETHACRIDINE LACTATE; HIGH DENSITY LIPOPROTEIN; LIPOPROTEIN; POLYPEPTIDE; TRYPSIN;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DIGESTION; ENZYME IMMOBILIZATION; MEMBRANE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

ANIMALS; APOLIPOPROTEIN A-I; ENZYMES, IMMOBILIZED; ESTERASES; LIPOPROTEINS, HDL; LIVER; MICE; TRYPSIN;

EID: 84866756765     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2012.07.032     Document Type: Article

References (13)

1. Iglesias A., Arranz M., Alvarez J.J., Perales J., Villar J., Herrera E., Lasuncion M.A. Choleteryl ester transfer activity in liver disease and cholestasis, and its relation with fatty acid composition of lipoprotein lipids. Clin. Chim. Acta 1996, 248:157-174.
2. Zhu H.L., Atkinson D. Conformation and lipid binding of a C-terminal (198-243) of human apolipoprotein A-1 (apo A-1). Biochemistry 2007, 46:1624-1634.
3. Park J.H., Park S.M., Park K.H., Cho K.H., Lee S.T. Analysis of apolipoprotein A-I as a substrate for matrix metalloproteinase-14. Biochem. Biophys. Res. Commun. 2011, 409:58-63.
4. Shimazaki Y., Watanabe S. Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis. Clin. Chim. Acta 2008, 390:145-147.
5. Shimazaki Y., Kuroda T. Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane. Biotechnol. Lett. 2009, 31:1545-1549.
6. Sakikawa T., Shimazaki Y. Reversible inhibition of esterase activity after separation and immobilization. Appl. Biochem. Biotechnol. 2011, 165:69-74.
7. Liu Y., Lu H., Zhong W., Song P., Kong J., Yong P., Girault H.H., Lui B. Multilayer-assembled microchip for enzyme immobilization as reactor toward low-level protein identification. Anal. Chem. 2006, 78:801-808.
8. Xu F., Wang W.H., Tan Y.J., Bruening M.L. Facile trypsin immobilization in polymeric membranes for rapid, efficient protein digestion. Anal. Chem. 2010, 82:10045-10051.
9. Zhou Y., Yi T., Park S.S., Chadwick W., Shen R.F., Wu W.W., Martin B., Maudsley S. Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor. J. Proteomics 2011, 74:1030-1035.
10. Bencharit S., Morton C.L., Hyatt J.L., Kuhn P., Danks M.K., Potter P.M., Redinbo M.R. Crystal structure of human carboxylesterase 1 complexed with the Alzheimer's drug tacrine: from binding promiscuity to selective inhibition. Chem. Biol. 2003, 10:341-349.
11. Shimazaki Y., Sugawara Y., Ohtsuka Y., Manabe T T. Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis. Proteomics 2003, 3:2002-2007.
12. Shimazaki Y., Sakikawa T. Determination of malic acid using a malate dehydrogenase reactor after purification and immobilization in non-denaturing conditions and staining with Ponceau S. Protein Peptide Lett. 2010, 17:1048-1052.
13. Hossain M.A., Ngeth S., Chan T., Oda M.N., Fracis G.A. Lipid-bound apolipoproteins in tyrosyl radical-oxidized HDL stabilize ABCA1 like lipid-free apolipoprotein A-I. BMC Biochem. 2012, 13:1-11.