Evaluation of genotoxic effects of lead in pottery-glaze workers using micronucleus assay, alkaline comet assay and DNA diffusion assay

International Archives of Occupational and Environmental Health

Volumn 85, Issue 7, 2012, Pages 807-818

  Kasuba V ^a   Rozgaj, R ^a   Milic M ^a   Zeljezic D ^a   Kopjar, N ^a   Pizent, A ^a   Kljakovic Gaspic Z ^a   Jazbec, A ^b  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

^b UNIVERSITY OF ZAGREB   (Croatia)

Author keywords

Alkaline comet assay; Biological markers; Ceramic workers; Lead exposure; Micronucleus assay

Indexed keywords

CADMIUM; CYTOCHALASIN B; DNA; LEAD; MUTAGENIC AGENT;

ADOLESCENT; ADULT; ARTICLE; BLOOD; CELL CYCLE; CERAMICS; CHEMISTRY; COMET ASSAY; DNA FRAGMENTATION; DRUG EFFECT; FEMALE; GENETICS; HUMAN; LEAD POISONING; LYMPHOCYTE; MALE; MICRONUCLEUS; MICRONUCLEUS TEST; MIDDLE AGED; OCCUPATIONAL DISEASE; OCCUPATIONAL EXPOSURE;

ADOLESCENT; ADULT; CADMIUM; CELL CYCLE; CERAMICS; COMET ASSAY; CYTOCHALASIN B; DNA; DNA FRAGMENTATION; FEMALE; HUMANS; LEAD; LEAD POISONING; LYMPHOCYTES; MALE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MIDDLE AGED; MUTAGENS; OCCUPATIONAL DISEASES; OCCUPATIONAL EXPOSURE; YOUNG ADULT;

EID: 84866731403     PISSN: 03400131     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00420-011-0726-4     Document Type: Article

References (89)

1. Ademuyiwa O, Ugbaja RN, Ojo DA, Owoigbe AO, Adeokun SE (2005) Reversal of aminolevulinic acid dehydratase (ALAD) inhibition and reduction of erythrocyte protoporphyrin levels by Vitamin C in occupational lead exposure in Abeokuta. Nigeria Environ Tox Pharmacol 20:404-411
2. Agency for toxic substances and disease registry (ATSDR) (1999) Toxicological profile for Lead. US Department of Health and Human Services, Atlanta
3. Alessio L, Castoldi MR, Odone P, Franchini I (1981) Behaviour of indicators of exposure and effect after cessation of occupational exposure to lead. Br J Ind Med 38:262-267
4. Al-Hakkak Z S H, Hamamy HA, Murad A M B, Hussain AF (1986) Chromosome aberrations in workers at a storage battery plant in Iraq. Mutat Res 171:53-60
5. ARC (Seventh Annual report on carcinogens) (1994) National toxicology program. National Institute of environmental Health Sciences. Research Triangle Park, North Carolina
6. Asomaning K, Miller DP, Liu G, Wain JC, Lynch TJ, Su L, Christiani DC (2008) Second hand smoke, age of exposure and lung cancer risk. Lung Cancer 61:13-20
7. Barale R, Marrazzini A, Bacci E, Disibio A, Tessa A, Cocchi L, Scarcelli V, Lubrano V, Vassalle C, Landi S (1998) Sister chromatid exchange and micronucleus frequency in human lymphocytes of 1650 subjects in an Italian population: I. Contribution of methodological factors. Environ Mol Mutagen 31:218-227
8. Basaran N, Shubair M, Ündeger Ü, Kars A (2003) Monitoring of DNA damage in foundry and pottery workers exposed to silica by the alkaline comet assay. Am J Ind Med 43:602-610
9. Beetstra S, Thomas P, Salisbury C, Turner J, Fenech M (2005) Folic acid deficiency increases chromosomal instability, chromosome 21 aneuploidy and sensitivity to radiation-induced micronuclei. Mutat Res 578:317-326
10. Berlin A, Schaller H (1974) European method for determination of deltaamino-levulinic acid dehydratase activity in blood. Z Clin Chem Clin Biochim 2:389-390
11. Bermúdez E, Stone K, Carter KM, Pryor WA (1994) Environmental tobacco smoke is just as damaging to DNA as mainstream smoke. Environ Health Perspect 102:870-874
12. Betti C, Davini T, Giannessi L, Loprieno N, Barale R (1995) Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat Res 343:201-207
13. Bolognesi C, Lando C, Forni, Landini E, Scarpato R, Migliore L, Bonassi S (1999) Chromosomal damage and aging: effect on micronuclei frequency in peripheral blood lymphocytes. Age Aging 28:393-397
14. Bonassi S, Ceppi M, Fontana V, Merlo F (1997) Multiple regression analysis of cytogenetic human data. Mutat Res 313:69-80
15. Bonassi S, Neri M, Lando C, Ceppi M, Lin Y, Chang WP, Holland N, Kirsch-Volders M, Wushou PC, Zeiger E, Fenech M (2003) The HUMN collaborative group, effect of smoking habit on the frequency of micronuclei in human lymphocytes: results from the human MicroNucleus project. Mutat Res 543:155-166
16. Calderón-Ezquerro C, Guerrero-Guerra C, Sansores-Martínez R, Calderón-Segura ME, Villalobos-Pietrini R, Amador-Muñoz O, Gómez-Arroyo S (2010) Genotoxicity in lymphocytes of smokers living in México City. Rev Int Contam Ambient 26:47-63
17. Chen Z, Jianlin L, Shijie C, Wei Z, Wei W, Lifen J, Hongping D, Jiliang H (2006) Evaluating the genotoxic effects of workers exposed to lead using micronucleus assay, comet assay and TCR gene mutation test. Toxicology 223:219-226
18. Chislom JJ, Brown DH (1975) Micro-scale photofluorimetric determination of "free erythrocyte protoporphyrin" (protoporphyrin IX). Clin Chem 21:1669
19. Collins AR, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, Fillion F, Panayiotidis M, Raslova K, Vaughan N (1997) Comet assay in human biomonitoring studies: reliability, validation, and applications. Environ Mol Mutagen 30:139-146
20. Dandevi K, Rozati R, Banu BS, Rao PH, Grover P (2003) DNA damage in workers exposed to lead using comet assay. Toxicology 187:183-193
21. De Restrepo HG, Sicard D, Torres MM (2000) DNA damage and repair in cells of lead exposed people. Am J Ind Med 38:330-334
22. Eastmond DA, Tucker JD (1989) Identification of aneuploidy-inducing agents using cytokinesis-blocked human lymphocyttes and antikinetochore antibody. Environ Mol Mutagen 13:34-43
23. Ergurhan-Ilhan I, Cadir B, Koyuncu-Arslan M, Arslan C, Gultepe FM, Ozkan G (2008) Level of oxidative stress and damage in erythrocytes in apprentices indirectly exposed to lead. Pediatr Int 50:45-50
24. Fenech M (2000) The in vitro micronucleus technique. Mutat Res 455:81-95
25. Fenech M (2007) Cytokinesis-block micronucleus cytome assay. Nat Protoc 2:1084-1104
26. Fenech M, Bonassi S (2011) The effect of age, gender, diet and life style on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes. Mutagenesis 26(1):43-49
27. Fenech M, Morley AA (1985) Measurement of micronuclei in lymphocytes. Mutat Res 147:29-36
28. Fenech M, Morley AA (1986) Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mutat Res 161:193-198
29. Fenech M, Rinaldi J (1994) The relationship between micronuclei in human lymphocytes and plasma level of vitamin C, vitamin E, vitamin B12 and folic acid. Carcinogenesis 15:1405-1411
30. Fenech M, Neville S, Rinaldi J (1994) Sex is important variable affecting spontaneous micronucleus frequency in cytokinesis-blocked lymphocytes. Mutat Res 313:203-207
31. Fenech MF, Dreosti LE, Rinaldi JR (1997) Folate, vitamin B12, homocysteine status and chromosome damage rate in lymphocytes of older men. Carcinogenesis 18:1329-1336
32. Forni A, Camiaghi G, Sechi GC (1976) Initial occupational exposure to lead: chromosome and biochemical Wndings. Arch Environ Health 31:73-78
33. Fracasso ME, Perbellini L, Solda S, Talamini G, Franceschetti P (2002) Lead induced DNA strand breaks in lymphocytes of exposed workers: role of reactive oxygen species and protein kinase C. Mutat Res 515:159-169
34. Frome EL, Du Frain RJ (1986) Maximum likelihood estimation for cytogenetic dose-response curves. Biometrics 42:73-84
35. Ganguly BB (1993) Cell division, chromosomal damage and micronucleus formation in peripheral lymphocytes of healthy donors: related to donors age. Mutat Res 295:135-148
36. Garcia-Leston J, Laffon B, Roma-Torres J, Teixeira JP, Costa C, Coelho P, Monteiro S, Mayan O, Valdiglesias V, Pasaro E, Mendez J (2009) Evaluation of genotoxic effects of occupational exposure to lead bay means of the comet assay. Abstract book of ICEM 2009, August 20-25, Firenze, Italy, pp 287-288
37. Garcia-Leston J, Mendez J, Pasaro E, Laffon B (2010) Genotoxic effects of lead: an updated review. Environ Int 36:623-636
38. Grover PK, Danadevi K, Mahboob M, Rozati R, Saleha B, Rahman MF (2003) Evaluation of genetic damage in workers employed in pesticide production utilizing comet assay. Mutagenesis 18:201-205
39. Hamurcu Z, Donmez H, Saraymen R, Demirtas H (2001) Micronucleus frequencies in workers exposed to Lead, Zinc, and Cadmium. Biol Trace Elem Res 83:97-102
40. Hartmann A, Fender H, Speit G (1998) A comparative biomonitoring study of workers of a waste disposal site using cytogenetic tests and the comet (single cell gel) assay. Environ Mol Mutagen 32:17-24
41. Hartwig A (1994) Role of DNA repair inhibition in lead-And cadmium-induced genotoxicity: a review. Environ Health Perspect 102:45-50
42. Hartwig A, Schlepergrell R, Beyersmann D (1990) Indirect mechanism of lead induced genotoxicity in cultured mammalian cells. Mutat Res 241:75-82
43. Hoffmann M, Hagberg S, Karlsson A, Nilsson R, Ranstam J, Hogstedt B (1984) Inorganic lead exposure does not effect lymphocyte micronuclei in car radiator repair workers. Hereditas 101:223-226
44. Hogstedt C, Hane M, Agrell A, Bodin L (1983) Neuropsychological test results and symptoms among workers with well-defined longterm exposure to lead. Br J Ind Med 40:99-105
45. Huang P, Huang B, Weng H, Nakayama K, Morimoto K (2009) Effects of lifestyle on micronuclei frequency in human lymphocytes in Japanese hard-metal workers. Prev Med 48:383-388
46. Huber R, Streng S, Bauchinger M (1983) The suitability of the human lymphocyte micronucleus assay system for biological dosimetry. Mutat Res 111:185-193
47. Johansson L, Pelliccari CE (1988) Lead induced changes in the stabilization of the mouse sperm chromatin. Toxicology 51:11-24
48. Johnson FM (1998) The genetic effects of environmental lead. Mutat Res 410:123-140
49. Jurasovic J, Telisman S (1993) Determination of lead and cadmium in human seminal fluid by electrothermal atomic absorption spectrometry. J Anal At Spectrom 8:419-425
50. Kasuba V, Rozgaj R, Milic M, Zeljezic D, Kopjar N, Pizent A, Kljakovic-Gaspic Z (2009) Evaluation of lead exposure in battery-manufacturing workers with focus on different biomarkers. J Appl Tox 30:321-328
51. Kazimirova A, Barancokova M, Krajcovicova-Kudlackova M, Volkovova K, Staruchova M, Valachovicova M, Paukova V, Blazicek P, Wsolova L, Dusinska M (2006) The relationship between micronuclei in human lymphocytes and selected micronutrients in vegetarians and non-vegetarians. Mutat Res 611:64-70
52. Kelada SN, Shelton E, Kaufmann RB, Khoury MJ (2001) A-Aminolevulinic acid dehydratase genotype and lead toxicity: a HuGE. Am J Epidemiol 154:1-13
53. Manikantan P, Balachander V, Sasikala K (2010) DNA damage in workers occupationally exposed to lead, using comet assay. Int J Biol 2:103-110
54. Mantere P, Hanninen H, Hernberg S, Luukkonen R (1984) A prospective follow-up study on psychological effects in workers exposed to low levels of lead. Scand J Work Environ Health 10:43-50
55. McKevith B (2004) Folate and folic acid. British Nutrition Foundation, www.nutrition.org.uk
56. Migliore L, Parrini M, Sbrana I, Biagini C, Battaglia A, Loprieno N (1991) Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect. Mutat Res 256:13-20
57. Milić M, Kašuba V, Oreščanin V, Želježić D, Kopjar N, Rozgaj R (2008) Chromosome damage in workers in cigarette manufacturing industry. J Appl Tox 28:399-404
58. Minozzo R, Deimling LJ, Petrucci Gigante L, Santos-Mello R (2004) Micronuclei in peripheral blood lymphocytes of workers exposed to lead. Mutat Res 565:53-60
59. MRC Vitamin Study Research Group (1991) Prevention of neural tube defects: Results of the Medical Researc Council Vitamin Study. Lancet 338:131-137
60. Murata K, Iwata T, Dakeishi M, Karita K (2009) Lead toxicity: does the critical level of lead resulting in adverse effects differ between adults and children? J Occup Health 51:1-12
61. OSHA (2002) Employee Standard Summary-1910.1025 App B. U. S. Department of Labor, Occupational Health and Safety Administration. Available at: http://www.osha.gov/pls/oshaweb/ovaolisp. show-document?-table=STANDARDS&p- id=10032
62. Palus J, Dziubaltowska E, Rydzynski K (1999) DNA damage detected by the comet assay in the white blood cells of workers in a wooden furniture plant. Mutat Res 444:61-74
63. Palus J, Rydzynski K, Dziubaltowska E, Wyszynska K, Natarajan AK, Nilsson R (2003) Genotoxic effects of occupational exposure to lead and cadmium. Mutat Res 540:19-28
64. Pinto D, Ceballos JM, García G, Guzmán P, Del Razo LM, Vera E, Gómez H, García A, Gonsebatt ME (2000) Increased cytogenetic damage in outdoor painters. Mutat Res 467:105-111
65. Popović M, McNeill FE, Chettle DR, Webber CE, Lee CV, Kaye WE (2005) Impact of occupational exposure on lead levels in women. Environ Med 113:478-484
66. Rozgaj R, Kašuba V, Jazbec A (2001) Preliminary study of cytogenetic damage in personnel exposed to anesthetic gases. Mutagenesis 16:139-143
67. Sarto F, Stella M, Acqua A (1978) Cytogenetic studies in twenty workers occupationally exposed to lead (in Italian). Med Lav 69:120-180
68. ® user's guide, Version 8. SAS Institute Inc, Cary
69. Schwartz J, Landrigan PJ, Baker EL Jr, Orenstein WA, von Lindern IH (1990) Lead-induced anaemia: dose-response relationships and evidence for a threshold. Am J Pub Health 80:165-168
70. Seppäläinen AM, Hernberg S, Vesanto R, Kock B (1983) Early neurotoxic effects of occupational lead exposure: a prospective study. Neurotoxicology 4:181-192
71. Shaik AP, Jamil K (2009) Individual susceptibility and genotoxicity in workers exposed to hazardous materials like lead. J Hazard Mater 168:918-924
72. Silbergeld EK, Waalkes M, Rice JM (2000) Lead as carcinogen: experimental evidence and mechanisms of action. Am J Ind Med 38:316-323
73. Singh NP (2000) A simple method for accurate estimation of apoptotic cells. Exp Cell Res 256:328-337
74. Singh NP (2005) Apoptosis assessment by the DNA diffusion assay. Chemosensitivity Vol. 2: In vivo models. Imaging Mole Regulat 111:55-67
75. Singh N, McCoy M, Tice R, Schneider E (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191
76. Stoleski S, Karadžinska-Bislimovska J, Stikova E, Risteska-Kuc S, Mijakovski D, Minov J (2008) Adverse effects in workers exposed to inorganic lead. Arch Ind Hyg Toxicol 59:19-29
77. Swanson DA, Liu MJ, Baker PJ, Garrett L, Stitzel M, Wu J, Harris M, Banerjee R, Shane B, Brody LC (2001) Targeted disruption of the methionine synthase gene in mice. Mol Cell Biol 21:1058-1065
78. Telišman S, Keršanc A, Prpić-Majić D (1982) The relevance of arguments for excluding ALAD from the recommended biological limit values in occupational exposure to inorganic lead WHO 1980. Int Arch Occup Environ Health 50:397-412
79. Telišman S, Prpić-Majić D, Keršanc A (1990) Relationships between blood lead and indicators of effect in cows environmentally exposed to lead. Toxicol Lett 52:347-356
80. Thierens H, Vral A, De Ridder L (1996) A cytogenetic study of radiological workers: effect of age, smoking and radiation burden on the micronucleus frequency. Mutat Res 360:75-82
81. Vaglenov A, Creus A, Laltchev S, Petkova V, Pavlova S, Marcos R (2001) Occupational exposure to lead and induction of genetic damage. Environ Health Perspect 109:295-298
82. Wagner C (1995) Biochemical role of folate in cellular metabolism. In: Bailey LB (ed) Folate in health and disease. Marcel Dekker, New York, pp 23-42
83. Wetmur JG (1994) Influence of the common human delta-Aminolevulinate dehydratase polymorphism on lead body burden. Environ Health Perspect 102(Suppl. 3):215-219
84. Woźniak K, Blasiak J (2003) In vitro genotoxicity of lead-Acetate; induction of single and double DNA strand breaks and DNA-protein cross-links. Mutat Res 535:127-139
85. Zalata A, Yahia S, El-Bakary A, Elsheikha HM (2007) Increased DNA damage in children caused by passive smoking as assessed by comet assay and oxidative stress. Mutat Res 629:140-147
86. Zhijian C, Jianlin L, Shije C, Wei Z, Wei W, Lifen J, Hongping D, Jiliang H (2006) Evaluating the genotoxic effects of workers exposed to lead using micronucleus assay, comet assay and TRC gene mutation test. Toxicology 223:219-226
87. Zhou W, Heist RS, Liu G, Asomaning K, Miller DP, Neuberg DS, Wain JC, Lynch TJ, Christiani DC (2006) Second hand smoke exposure and survival in early-stage non-small-cell lung cancer patients. Clin Cancer Res 12:7187-7193
88. Zijno A, Andreoli C, Leopardi P, Marcon F, Rossi S, Caiola S, Verdina A, Galati R, Cafolla A, Crebelli R (2003) Folate status, metabolic genotype, and biomarkers of genotoxicity in healthy subjects. Carcinogenesis 24:1097-1103
89. Zing JM, Jones PA (1997) Genetic and epigenetic aspects of DNA methylation on genome expression, evolution, mutation and carcinogenesis. Carcinogenesis 18:869-882