An RNAi-Based genomic library for forward genetics in the African trypanosome

Gene Silencing by RNA Interference: Technology and Application

Volumn , Issue , 2004, Pages 241-257

  Morris, James C ^a   Wang, Zefeng ^b   Motyka, Shawn A ^c   Drew, Mark E ^d   Englund, Paul T ^c  

^a CLEMSON UNIVERSITY   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84866715134     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1201/9780203489253     Document Type: Chapter

References (27)

1. Vickerman, K., Developmental cycles and biology of pathogenic trypanosomes. Br. Med. Bull., 41, 105, 1985.
2. Ferguson, M.A.J. et al., Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane. Science, 239, 753, 1988.
3. Masterson, W.J. et al., A novel pathway for glycan assembly: biosynthesis of the glycosylphosphatidylinositol anchor of the trypanosome variant surface glycoprotein. Cell, 56, 793, 1989.
4. Simpson, L., S. Sbicego, and R. Aphasizhev, Uridine insertion/deletion RNA editing in trypanosome mitochondria: a complex business. RNA, 9, 265, 2003.
5. Boothroyd, J.C. and G.A. Cross, Transcripts coding for variant surface glycoproteins of Trypanosoma brucei have a short, identical exon at their 5' end. Gene, 20, 281, 1982.
6. Mair, G. et al., A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA. RNA, 6, 163, 2000.
7. Wirtz, E. et al., A tightly regulated inducible expression system for conditional gene knockouts and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol., 99, 89, 1999.
8. Seebeck, T. et al., Tubulin genes of Trypanosoma brucei: a tightly clustered family of alternating genes. Proc. Natl. Acad. Sci. USA, 80, 4634, 1983.
9. Thomashow, L.S. et al., Tubulin genes are tandemly linked and clustered in the genome of Trypanosoma brucei. Cell, 32, 35, 1983.
10. Bringaud, F. and T. Baltz, Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei. Mol. Cell Biol., 13, 1146, 1993.
11. Sanchez, M.A. et al., Six related nucleoside/nucleobase transporters from Trypanosoma brucei exhibit distinct biochemical functions. J. Biol. Chem., 277, 21499, 2002.
12. Ngo, H., C. Tschudi, K. Gull, and E. Ullu, Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc. Natl. Acad. Sci. USA, 95, 14687, 1998.
13. Bastin, P., T. Sherwin, and K. Gull, Paraflagellar rod is vital for trypanosome motility. Nature, 391, 548, 1998.
14. LaCount, D.J., B. Barrett, and J.E. Donelson, Trypanosoma brucei FLA1 is required for flagellum attachment and cytokinesis. J. Biol. Chem., 277, 17580, 2002.
15. Chen, Y. et al., Development of RNA interference revertants in Trypanosoma brucei cell lines generated with a double stranded RNA expression construct driven by two opposing promoters. Mol. Biochem. Parasitol., 126, 275, 2003.
16. Wang, Z. et al., Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem., 275, 40174, 2000.
17. El-Sayed, N.M. et al., The African trypanosome genome. Int. J. Parasitol., 30, 329, 2000.
18. Morris, J.C. et al., Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library. EMBO J., 21, 4429, 2002.
19. Drew, M.E. et al., The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNAi library. J. Biol. Chem., 278, 465ab 2003.
20. Morris, J.C. et al., Inhibition of bloodstream form Trypanosoma brucei gene expression by RNA interference using the pZJM dual T7 vector. Mol. Biochem. Parasitol., 117, 111, 2001.
21. Wirtz, E. and C. Clayton, Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science, 268, 1179, 1995.
22. van den Hoff, M.J., A.F. Moorman, and W.H. Lamers, Electroporation in “intracellular” buffer increases cell survival. Nucleic Acids Res., 20, 2902, 1992.
23. Hirumi, H. and K. Hirumi, Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers. J. Parasitol., 75, 985, 1989.
24. Brun, R. and M. Shonenberger, Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Tropica, 36, 289, 1979.
25. Shi, H. et al., Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA, 6, 1069, 2000.
26. Timmons, L. and A. Fire, Specific interference by ingested dsRNA. Nature, 395, 854, 1998.
27. Tzfati, Y. et al., A single-stranded DNA-binding protein from Crithidia fasciculata recognizes the nucleotide sequence at the origin of replication of kinetoplast DNA minicircles. Proc. Natl. Acad. Sci. USA, 89, 6891, 1992.