A peptide-based biosensor assay to detect intracellular Syk kinase activation and inhibition

Biochemistry

Volumn 51, Issue 38, 2012, Pages 7515-7524

  Lipchik, Andrew M ^a   Killins, Renee L ^a   Geahlen, Robert L ^a   Parker, Laurie L ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION STATE; ARTIFICIAL PEPTIDES; B CELLS; CELL-BASED ASSAYS; CELL-PERMEABLE PEPTIDES; COMPLEX RELATIONSHIPS; DIRECT DETECTION; DRUG ACTION; INTACT CELLS; KINASE ACTIVATION; LABORATORY CELLS; LIVE CELL; PHOSPHORYLATION SITES; PRIMARY CELLS; RHEUMATOID ARTHRITIS; SCAFFOLDING PROTEINS; SPLEEN TYROSINE KINASE; THERAPEUTIC TARGETS;

AMINO ACIDS; BIOSENSORS; CELL CULTURE; CELLS; CHEMICAL ACTIVATION; DISEASES; PEPTIDES; PHOSPHORYLATION; PHYSIOLOGICAL MODELS; SUBSTRATES;

BIOASSAY;

B LYMPHOCYTE ANTIGEN; HYDROGEN PEROXIDE; IMMUNOGLOBULIN M; PROTEIN KINASE SYK;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; B LYMPHOCYTE; BURKITT LYMPHOMA; CANCER CELL; CELL CULTURE; ENZYME ACTIVATION; ENZYME INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYMIC BIOSENSOR; IN VITRO STUDY; MOUSE; NONHUMAN; OXIDATIVE STRESS; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; SASTIDE BIOSENSOR; SPLEEN CELL;

ANIMALS; B-LYMPHOCYTES; BIOSENSING TECHNIQUES; CELL LINE; ENZYME ACTIVATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; PEPTIDES; PHOSPHORYLATION; PROTEIN-TYROSINE KINASES; SPECTROMETRY, FLUORESCENCE;

EID: 84866695243     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300970h     Document Type: Article

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