A fast and simple method for probing the interaction of peptides and proteins with lipids and membrane-mimetics using GB1 fusion proteins and NMR spectroscopy

Protein Science

Volumn 21, Issue 10, 2012, Pages 1566-1570

  Sommer, Lisa A M ^a   Meier, Melanie A ^a   Dames, Sonja A ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Membrane mimetic; NMR spectroscopy; Protein lipid interactions; Protein membrane interactions

Indexed keywords

B1 DOMAIN OF STREPTOCOCCAL PROTEIN G; HYBRID PROTEIN; LIPID; LIPOSOME; UNCLASSIFIED DRUG;

ARTICLE; MICELLE; MOLECULAR PROBE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LIPID INTERACTION; PROTEIN PURIFICATION;

BACTERIAL PROTEINS; MAGNETIC RESONANCE SPECTROSCOPY; MEMBRANE LIPIDS; MEMBRANE PROTEINS; MEMBRANES, ARTIFICIAL; PEPTIDES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

EID: 84866600111     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2127     Document Type: Article

References (23)

1. Di Paolo G, De Camilli P (2006) Phosphoinositides in cell regulation and membrane dynamics. Nature 443:651-657. (Pubitemid 44564702)
2. Yeung T, Ozdamar B, Paroutis P, Grinstein S (2006) Lipid metabolism and dynamics during phagocytosis. Curr Opin Cell Biol 18:429-437. (Pubitemid 44016055)
3. Botelho RJ, Grinstein S (2011) Phagocytosis. Curr Biol 21:R533-538.
4. Navarro-Lerida I, Sanchez-Perales S, Calvo M, Rentero C, Zheng Y, Enrich C, Del Pozo MA (2012) A palmitoylation switch mechanism regulates Rac1 function and membrane organization. EMBO J 31:534-551.
5. Xian W, Janmey PA (2002) Dissecting the gelsolin-polyphosphoinositide interaction and engineering of a polyphosphoinositide-sensitive gelsolin C-terminal half protein. J Mol Biol 322:755-771.
6. Kumar N, Zhao P, Tomar A, Galea CA, Khurana S (2004) Association of villin with phosphatidylinositol 4,5-bisphosphate regulates the actin cytoskeleton. J Biol Chem 279:3096-3110. (Pubitemid 38114303)
7. Goult BT, Bouaouina M, Elliott PR, Bate N, Patel B, Gingras AR, Grossmann JG, Roberts GC, Calderwood DA, Critchley DR, Barsukov IL (2010) Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation. EMBO J 29:1069-1080.
8. Dames SA, Junemann A, Sass HJ, Schonichen A, Stopschinski BE, Grzesiek S, Faix J, Geyer M (2011) Structure, dynamics, lipid binding, and physiological relevance of the putative GTPase-binding domain of Dictyostelium formin C. J Biol Chem 286:36907-36920.
9. Jacinto E, Hall MN (2003) Tor signalling in bugs, brain and brawn. Nat Rev Mol Cell Biol 4:117-126. (Pubitemid 36172695)
10. Wullschleger S, Loewith R, Hall MN (2006) TOR signaling in growth and metabolism. Cell 124:471-484. (Pubitemid 43199434)
11. Dames SA, Mulet JM, Rathgeb-Szabo K, Hall MN, Grzesiek S (2005) The solution structure of the FATC domain of the protein kinase target of rapamycin suggests a role for redox-dependent structural and cellular stability. J Biol Chem 280:20558-20564. (Pubitemid 40776757)
12. Dames SA (2010) Structural basis for the association of the redox-sensitive target of rapamycin FATC domain with membrane-mimetic micelles. J Biol Chem 285:7766-7775.
13. Huth JR, Bewley CA, Jackson BM, Hinnebusch AG, Clore GM, Gronenborn AM (1997) Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR. Protein Sci 6:2359-2364. (Pubitemid 27490746)
14. Koenig BW, Rogowski M, Louis JM (2003) A rapid method to attain isotope labeled small soluble peptides for NMR studies. J Biomol NMR 26:193-202. (Pubitemid 36758440)
15. Gronenborn AM, Filpula DR, Essig NZ, Achari A, Whitlow M, Wingfield PT, Clore GM (1991) A novel, highly stable fold of the immunoglobulin binding domain of streptococcal protein G. Science 253:657-661. (Pubitemid 21917198)
16. Rodriguez Camargo DC, Link NM, Dames SA (2012) The FKBP-rapamycin binding domain of human TOR undergoes strong conformational changes in the presence of membrane-mimetics with and without the regulator phosphatidic acid. Biochemistry 51:4909-4921.
17. Warschawski DE, Arnold AA, Beaugrand M, Gravel A, Chartrand E, Marcotte I (2011) Choosing membrane mimetics for NMR structural studies of transmembrane proteins. Biochim Biophys Acta 1808:1957-1974.
18. Tieleman DP, van der Spoel D, Berendsen HJC (2000) Molecular dynamics simulations of dodecylphosphocholine micelles at three different aggregate sizes: micellar structure and chain relaxation. J Phys Chem B 104:6380-6388.
19. Sanders CR, Sonnichsen F (2006) Solution NMR of membrane proteins: practice and challenges. Magn Reson Chem 44:S24-S40. (Pubitemid 44090388)
20. Stafford RE, Fanni T, Dennis EA (1989) Interfacial properties and critical micelle concentration of lysophospholipids. Biochemistry 28:5113-5120. (Pubitemid 19162760)
21. Hauser H (2000) Short-chain phospholipids as detergents. Biochim Biophys Acta 1508:164-181.
22. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277-293.
23. Johnson BA (2004) Using NMRView to visualize and analyze the NMR spectra of macromolecules. Methods Mol Biol 278:313-352.