Embryonic stem cell test: Stem cell use in predicting developmental cardiotoxicity and osteotoxicity

Methods in Molecular Biology

Volumn 889, Issue , 2012, Pages 147-179

  Kuske, Béatrice ^a   Pulyanina, Polina Y ^a   Nieden, Nicole I Zur ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Developmental cardiotoxicity; Developmental osteotoxicity; Embryonic stem cell test; Embryotoxicity; Pluripotent stem cells; Teratogen

Indexed keywords

COMPLEMENTARY DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MYOSIN HEAVY CHAIN ALPHA; OSTEOCALCIN; RNA 18S; TOXIC SUBSTANCE; TERATOGENIC AGENT;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BONE DEVELOPMENT; BONE DISEASE; CARDIOTOXICITY; CELL ASSAY; CELL DIFFERENTIATION; CELL EXPANSION; CHEMOSENSITIVITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXICITY; DEVELOPMENTAL TOXICITY; EMBRYO; EMBRYO DEVELOPMENT; EMBRYONIC STEM CELL; EMBRYOTOXICITY; FIBROBLAST; GENE EXPRESSION PROFILING; HEART DEVELOPMENT; IC 50; IN VITRO STUDY; MOUSE; NONHUMAN; OSTEOTOXICITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THAWING; TOXICITY; TOXICITY TESTING; ANIMAL; CELL CULTURE; CULTURE TECHNIQUE; DEVELOPMENTAL BIOLOGY; DRUG EFFECT; FETUS MATURITY; HUMAN; METHODOLOGY; PHYSIOLOGY; PLURIPOTENT STEM CELL; REVIEW; TERATOLOGY;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; DEVELOPMENTAL BIOLOGY; EMBRYONIC STEM CELLS; FETAL ORGAN MATURITY; HUMANS; PLURIPOTENT STEM CELLS; TERATOGENS; TERATOLOGY; TOXICITY TESTS;

EID: 84866517272     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-867-2_10     Document Type: Article

References (27)

1. Gruber HE, Chow Y, Hoelscher GL, Ingram JA, Zinchenko N, Norton HJ, Sun Y, Hanley EN Jr (1976) Micromass culture of human anulus cells: morphology and extracellular matrix production. Spine 35(10): 1033-1038
2. Cuthbertson RA, Beck F (1990) Postimplantation whole embryo culture: a new method for studying ocular development. Invest Ophthalmol Vis Sci 31(8):1653-1656
3. Hermsen SA, van den Brandhof EJ, van der Ven LT, Piersma AH (2011) Relative embryotoxicity of two classes of chemicals in a modi fied zebra fish embryotoxicity test and comparison with their in vivo potencies. Toxicol In Vitro 25(3):745-753
4. Heuer J, Graeber IM, Pohl I, Spielmann H (1994) Culture system for the differentiation of murine embryonic stem cells-a new approach to in vitro testing for embryotoxicity and for developmental immunotoxicology. In: Fracchia GN (ed) European medicines research. IOS, Amsterdam, pp 134-145
5. Scholz G, Genschow E, Pohl I, Bremer S, Paparella M, Raabe H, Southee J, Spielmann H (1999) Prevalidation of the embryonic stem cell test (EST)-a new in vitro embryotoxicity test. Toxicol In Vitro 13(4-5):675-681
6. Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292(5819):154-156
7. Martin GR (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 78(12): 7634-7638
8. Doetschman TC, Eistetter H, Katz M, Schmidt W, Kemler R (1985) The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 87: 27-45
9. Wobus AM, Wallukat G, Hescheler J (1991) Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrener-gic and cholinergic agents and Ca 2+ channel blockers. Differentiation 48(3):173-182
10. Buttery LD, Bourne S, Xynos JD, Wood H, Hughes FJ, Hughes SP, Episkopou V, Polak JM (2001) Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng 7(1):89-99
11. Zur Nieden NI, Kempka G, Ahr HJ (2003) In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation 71(1):18-27
12. Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D (1988) Inhibition of pluripotential embryonic stem cell differentiation by puri fied polypeptides. Nature 336(6200):688-690
13. Niwa H, Burdon T, Chambers I, Smith A (1998) Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev 12(13):2048-2060
14. Hamazaki T, Oka M, Yamanaka S, Terada N (2004) Aggregation of embryonic stem cells induces Nanog repression and primitive endo-derm differentiation. J Cell Sci 117(Pt 23):5681-5686
15. Zur Nieden NI, Kempka G, Ahr HJ (2004) Molecular multiple endpoint embryonic stem cell test-a possible approach to test for the teratogenic potential of compounds. Toxicol Appl Pharmacol 194(3):257-269
16. Zur Nieden NI, Baumgartner L (2010) Assessing developmental osteotoxicity of chlorides in the embryonic stem cell test. Reprod Toxicol 30(2):277-283
17. Genschow E, Spielmann H, Scholz G, Seiler A, Brown N, Piersma A, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Becker K (2002) The ECVAM international validation study on in vitro embryotoxicity tests: results of the de finitive phase and evaluation of prediction models. European Centre for the Validation of Alternative Methods. Altern Lab Anim 30(2):151-176
18. Laschinski G, Vogel R, Spielmann H (1991) Cytotoxicity test using blastocyst-derived euploid embryonal stem cells: a new approach to in vitro teratogenesis screening. Reprod Toxicol 5:57-64
19. Spielmann H, Pohl I, Döring B, Liebsch M, Moldenhauer F (1997) The embryonic stem cell test (EST), an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. Toxicol In Vitro 10:119-127
20. Genschow E, Scholz G, Brown N, Piersma A, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Becker K, Spielmann H (2000) Development of prediction models for three in vitro embryotoxicity tests in an ECVAM validation study. Vitro Mol Toxicol 13(1): 51-66
21. Buesen R, Genschow E, Slawik B, Visan A, Spielmann H, Luch A, Seiler A (2009) Embryonic stem cell test remastered: comparison between the validated EST and the new molecular FACS-EST for assessing developmental toxicity in vitro. Toxicol Sci 108(2):389-400
22. Zur Nieden NI, Ruf LJ, Kempka G, Hildebrand H, Ahr HJ (2001) Molecular markers in embryonic stem cells. Toxicol In Vitro 15:455-461
23. Zur Nieden NI, Davis LA, Rancourt DE (2010) Comparing three novel endpoints for developmental osteotoxicity in the embryonic stem cell test. Toxicol Appl Pharmacol 247(2):91-97
24. Zur Nieden NI, Davis LA, Rancourt DE (2010) Monolayer cultivation of osteoprogeni-tors shortens duration of the embryonic stem cell test while reliably predicting developmental osteotoxicity. Toxicology 277(1-3):66-73
25. Adler S, Pellizzer C, Hareng L, Hartung T, Bremer S (2008) First steps in establishing a developmental toxicity test method based on human embryonic stem cells. Toxicol In Vitro 22(1):200-211
26. Hackenberg U, Bartling H (1959) Messen und Rechnen im pharmakologischen Laboratorium mit einem speziellen Zahlensystem (WL24-System). Arch Exp Pathol Pharmakol 235:437-463
27. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402-408