Comparing three novel endpoints for developmental osteotoxicity in the embryonic stem cell test

Toxicology and Applied Pharmacology

Volumn 247, Issue 2, 2010, Pages 91-97

  zur Nieden, Nicole I ^a,b,c   Davis, Lesley A ^a   Rancourt, Derrick E ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c FRAUNHOFER INSTITUTE FOR CELL THERAPY AND IMMUNOLOGY   (Germany)

Author keywords

Developmental osteotoxicity; Embryonic stem cell test; Embryonic stem cells; Embryotoxicity; Osteogenesis; Retinoic acid

Indexed keywords

ALKALINE PHOSPHATASE; CALCIUM; FLUOROURACIL; MESSENGER RNA; OSTEOCALCIN; PENICILLIN G; RETINOIC ACID;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BONE; CALCIFICATION; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; ENZYME ACTIVITY; GENE EXPRESSION; IMAGE ANALYSIS; IN VIVO STUDY; MORPHOMETRICS; MOUSE; NONHUMAN; OSTEOTOXICITY; QUANTITATIVE ANALYSIS; TERATOGENICITY;

EID: 77955278895     PISSN: 0041008X     EISSN: 10960333     Source Type: Journal    
DOI: 10.1016/j.taap.2010.05.010     Document Type: Article

References (41)

1. Adler S., Pellizzer C., Paparella M., Hartung T., Bremer S. The effects of solvents on embryonic stem cell differentiation. Toxicol. In Vitro 2006, 20(3):265-271.
2. Bigot K., De Lange J., Archer J., Clothier R., Bremer S. The relative semi-quantification of mRNA expression as a useful toxicological endpoint for the identification of embryotoxic/teratogenic substances. Toxicol. In Vitro 1999, 13:619-623.
3. Boucher D., Delost P. Developpement post-natal des descendents issus de meres traitees par la penicilline au cours de la gestion chez la souris. C.R. Soc. Biol. 1964, 158:528-532.
4. Bremer S., van Dooren M., Paparella M., Kolossov E., Fleischmann B., Hescheler J. Establishment of an in vitro embryotoxicity assay with green-fluorescent protein-expressing embryonic cell-derived cardiomyocytes. ATLA 1999, 27:471-484.
5. Bremer S., Worth A.P., Paparella M., Bigot K., Kolossov E., Fleischmann B.K., Hescheler J., Balls M. Establishment of an in vitro reporter gene assay for developmental cardiac toxicity. Toxicol. In Vitro 2001, 15(3):215-223. Jun.
6. Cormier J.T., zur Nieden N.I., Rancourt D.E., Kallos M.S. Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors. Tissue Eng. 2006, 12(11):3233-3245.
7. Dagg C.P. Sensitive stages for the production of developmental abnormalities in mice with 5-fluorouracil. Am. J. Anat. 1960, 106:89-96.
8. Denhardt D.T., Noda M. Osteopontin expression and function: role in bone remodeling. J. Cell. Biochem. 1998, Suppl 30-31:90-102.
9. Eichele G. Retinoids and vertebrate limb pattern formation. Trends Genet. 1989, 5:246-251.
10. Festag M., Viertel B., Steinberg P., Sehner C. An in vitro embryotoxicity assay based on the disturbance of the differentiation of murine embryonic stem cells into endothelial cells. II. Testing of compounds. Toxicol. In Vitro 2007, 21(8):1631-1640.
11. Genschow E., Scholz G., Brown N., Piersma A., Brady M., Clemann N., Huuskonen H., Paillard F., Bremer S., Becker K., Spielmann H. Development of prediction models for three in vitro embryotoxicity tests in an ECVAM validation study. In Vitro Mol. Toxicol. 2000, 13(1):51-66.
12. Genschow E., Spielmann H., Scholz G., Seiler A., Brown N., Piersma A., Brady M., Clemann N., Huuskonen H., Paillard F., Bremer S., Becker K. The ECVAM international validation study on in vitro embryotoxicity tests: results of the definitive phase and evaluation of prediction models. European Centre for the Validation of Alternative Methods. Altern. Lab. Anim. 2002, 30(2):151-176. Mar-Apr.
13. Genschow E., Spielmann H., Scholz G., Pohl I., Seiler A., Clemann N., Bremer S., Becker K. Validation of the embryonic stem cell test in the international ECVAM validation study on three in vitro embryotoxicity tests. Altern. Lab. Anim. 2004, 32(3):209-244.
14. 3 effects on alkaline phosphatase activity and parathyroid hormone responsiveness in an osteoblast-like osteosarcoma cell line. Endocrinology 1986, 119:932-939.
15. Heinonen O.P., Slone D., Shapiro S., Kaufman D.W. Birth Defects and Drugs in Pregnancy 1977, John Wright Publishing Sciences Group, Littleton, MA.
16. Huuskonen H. New models and molecular markers in evaluation of developmental toxicity. Toxicol. Appl. Pharmacol. 2005, 207(2 Suppl):495-500.
17. Kolossov E., Fleischmann B.K., Liu Q., Bloch W., Viatchenko-Karpinski S., Manzke O., Ji G.J., Bohlen H., Addicks K., Hescheler J. Functional characteristics of ES cell-derived cardiac precursor cells identified by tissue-specific expression of the green fluorescent protein. J. Cell Biol. 1998, 143:2045-2056.
18. Kramer J., Hegert C., Guan K., Wobus A.M., Müller P.K., Rohwedel J. Embryonic stem cell-derived chondrogenic differentiation in vitro: activation by BMP-2 and BMP-4. Mech. Dev. 2000, 92(2):193-205.
19. Laschinski G., Vogel R., Spielmann H. Cytotoxicity test using blastocyst-derived euploid embryonal stem cells: a new approach to in vitro teratogenesis screening. Reprod. Toxicol. 1991, 5:57-64.
20. Paparella M., Kolossov E., Fleischmann B.K., Hescheler J., Bremer S. The use of quantitative image analysis in the assessment of in vitro embryotoxicity endpoints based on a novel embryonic stem cell clone with endoderm-related GFP expression. Toxicol. In Vitro 2002, 16:589-597.
21. Pellizzer C., Adler S., Corvi R., Hartung T., Bremer S. Monitoring of teratogenic effects in vitro by analyzing a selected gene expression pattern. Toxicol. In Vitro 2004, 18:325-335.
22. Peters A.K., Steemans M., Hansen E., Mesens N., Verheyen G.R., Vanparys P. Evaluation of the embryotoxic potency of compounds in a newly revised high throughput embryonic stem cell test. Toxicol. Sci. 2008, 105(2):342-350.
23. Purpura K.A., Aubin J.E., Zandstra P.W. Two-color image analysis discriminates between mineralized and unmineralized bone nodules in vitro. Biotechniques 2003, 34(6):1188-1192.
24. Raouf A., Seth A. Ets transcription factors and targets in osteogenesis. Oncogene 2000, 19:6455-6463.
25. Scholz G., Pohl I., Seiler A., Bremer S., Brown N.A., Piersma A.H., Holzhütter H.G., Spielmann H. Results of the first phase of the ECVAM project "prevalidation and validation of three in vitro embryotoxicity tests". ALTEX 1998, 15(1):3-8. Article in German.
26. Scholz G., Pohl I., Genschow E., Klemm M., Spielmann H. Embryotoxicity screening using embryonic stem cells in vitro: correlation to in vivo teratogenicity. Cells Tissues Organs 1999, 165(3-4):203-211.
27. Scholz G., Genshow E., Pohl I., Bremer S., Paparella M., Raabe H., Southee J., Spielmann H. Prevalidation of the embryonic stem cell test (EST) - a new in vitro embryotoxicity test. Toxicol. In Vitro 1999, 13:675-681.
28. Seiler A., Visan A., Buesen R., Genschow E., Spielmann H. Improvement of an in vitro stem cell assay for developmental toxicity: the use of molecular endpoints in the embryonic stem cell test. Reprod. Toxicol. 2004, 18:231-240.
29. Shuey D.L., Lau C., Logson T.R., Zucker R.M., Elstein K.H., Narotsky M.G., Setzer R.W., Kavlock R.J., Rogers J.M. Biologically based dose-response modeling in developmental toxicology: biochemical and cellular sequelae of 5-fluorouracil exposure in the developing rat. Toxicol. Appl. Pharmacol. 1994, 126:129-144.
30. Smith M.K., Kimmel G.L., Kochhar D.M., Shepard S.P., Spielberg S.P., Wilson J.G. A selection of candidate compounds for in vitro teratogenesis test validation. Teratogen. Carcinogen. Mutagen. 1983, 3:461-480.
31. Spielmann H. Animal alternatives in Germany. Science 1997, 276(5309):19.
32. Spielmann H., Pohl I., Döring B., Moldenhauer F. In vitro embryotoxicity assay using two permanent cell lines: mouse embryonic stem cells and 3T3 fibroblasts. Abstracts of the 23 ETS conference 1995, Dublin. Teratology 1995, 51(6):31A-32A.
33. Spielmann H., Genschow E., Scholz G., Brown N.A., Piersma A.H., Brady M., Clemann N., Huuskonen H., Paillard F., Bremer S., Becker K. Preliminary results of the ECVAM validation study on three in vitro embryotoxicity tests. Altern. Lab. Anim. 2001, 29(3):301-303.
34. Stephens J.D., Golbus M.S., Miller T.R., Wilber R.R., Epstein C.J. Multiple congenital anomalies in a fetus exposed to 5-fluorouracil during the first trimester. Am. J. Obstet. Gynecol. 1980, 137:747-749.
35. Wobus A.M., Rohwedel J., Maltsev V., Hescheler J. Development of cardiomyocytes expressing cardiac-specific genes, action potentials, and ionic channels during embryonic stem cell-derived cardiogenesis. Ann. NY Acad. Sci. 1995, 752:460-469.
36. zur Nieden N.I., Ruf L.J., Kempka G., Hildebrand H., Ahr H.J. Molecular markers in embryonic stem cells. In vitro Toxicol. 2001, 15:455-461.
37. zur Nieden N.I., Kempka G., Ahr H.J. In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation 2003, 71(1):18-27.
38. zur Nieden N.I., Kempka G., Ahr H.J. Molecular multiple endpoint embryonic stem cell test - a possible approach to test for the teratogenic potential of compounds. Toxicol. Appl. Pharmacol. 2004, 194:257-269.
39. zur Nieden N.I., Kempka G., Rancourt D.E., Ahr H.J. Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: effect of cofactors on differentiating lineages. BMC Dev. Biol. 2005, 5:1.
40. zur Nieden N.I., Price F.D., Davis L.A., Everitt R.E., Rancourt D.E. Gene profiling on mixed embryonic stem cell populations reveals a biphasic role for beta-catenin in osteogenic differentiation. Mol. Endocrinol. 2007, 21(3):674-685.
41. zur Nieden N.I., Cormier J.T., Kallos M.S., Rancourt D.E., Rancourt D.E. Murine embryonic stem cells maintain pluripotency after long-term culture in suspension bioreactors. J. Biotechnol. 2007, 129(3):421-432.