A stepwise protocol to coat aAPC beads prevents out-competition of anti-CD3 mAb and consequent experimental artefacts

Journal of Immunological Methods

Volumn 385, Issue 1-2, 2012, Pages 90-95

  Fuertes Marraco, Silvia A ^a   Baumgaertner, Petra ^a   Legat, Amandine ^a   Rufer, Nathalie ^a   Speiser, Daniel E ^a  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

Author keywords

Artificial antigen presenting cells; Protein coating protocol; Quality control by flow cytometry; T cell activation

Indexed keywords

CD3 ANTIBODY; MOLECULAR SCAFFOLD; MONOCLONAL ANTIBODY; NANOBEAD;

ANTIGEN PRESENTING CELL; ARTICLE; CD8+ T LYMPHOCYTE; CONTROLLED STUDY; IMMUNOASSAY; IMMUNOLOGICAL SYNAPSE; MATERIAL COATING; PRIORITY JOURNAL; QUALITY CONTROL;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGEN-PRESENTING CELLS; ANTIGENS, CD3; ARTIFACTS; CD8-POSITIVE T-LYMPHOCYTES; FLOW CYTOMETRY; HUMANS; IMMUNOASSAY; LEUKOCYTES, MONONUCLEAR; MICE; MICROSPHERES; MUROMONAB-CD3;

EID: 84866514580     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.07.017     Document Type: Article

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