Evaluation of multiplexed PCR and liquid-phase array for identification of respiratory fungal pathogens

Medical Mycology

Volumn 50, Issue 7, 2012, Pages 775-780

  Buelow, Daelynn R ^a   Gu, Zhengming ^a   Walsh, Thomas J ^b   Hayden, Randall T ^a  

^a ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^b WEILL CORNELL MEDICAL CENTER   (United States)

Author keywords

Fungal detection; Luminex; Multiplex; PCR

Indexed keywords

ARTICLE; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; ASPERGILLUS ORYZAE; ASPERGILLUS TERREUS; CROSS REACTION; FUNGAL DETECTION; FUNGUS IDENTIFICATION; FUSARIUM SOLANI; LIMIT OF DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PAECILOMYCES LILACINUS; SCEDOSPORIUM APIOSPERMUM; SENSITIVITY AND SPECIFICITY;

CROSS REACTIONS; DNA PRIMERS; FUNGI; HUMANS; MICROARRAY ANALYSIS; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOLOGY; MYCOSES; RESPIRATORY TRACT DISEASES; SENSITIVITY AND SPECIFICITY;

ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; ASPERGILLUS ORYZAE; ASPERGILLUS TERREUS; FUNGI; FUSARIUM SOLANI; PAECILOMYCES LILACINUS; PSEUDALLESCHERIA BOYDII; RHIZOPUS MICROSPORUS;

EID: 84866484068     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.3109/13693786.2012.666681     Document Type: Article

References (17)

1. Stevens DA. Diagnosis of fungal infections: Current status. J Antimicrob Chemother 2002; 49 (Suppl. 1): 11-19.
2. Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev 2011; 24: 247-280.
3. Marr KA, Patterson T, Denning D. Aspergillosis. Pathogenesis, clinical manifestations, and therapy. Infect Dis Clin North Am 2002; 16: 875-894, v.i.
4. Bart-Delabesse E, Basile M, Al Jijakli A, et al . Detection of Aspergillus galactomannan antigenemia to determine biological and clinical implications of beta-lactam treatments. J Clin Microbiol 2005; 43: 5214-5220.
5. Pfeiffer CD, Fine JP, Safdar N. Diagnosis of invasive aspergillosis using a galactomannan assay: A meta-analysis. Clin Infect Dis 2006; 42: 1417-1427.
6. Odabasi Z, Mattiuzzi G, Estey E, et al . Beta-D-glucan as a diagnostic adjunct for invasive fungal infections: Validation, cutoff development, and performance in patients with acute myelogenous leukemia and myelodysplastic syndrome. Clin Infect Dis 2004; 39: 199-205.
7. Obayashi T, Yoshida M, Tamura H, et al . Determination of plasma (1- - 3)-beta-D-glucan: A new diagnostic aid to deep mycosis. J Med Vet Mycol 1992; 30: 275-280.
8. Weile J, Knabbe C. Current applications and future trends of molecular diagnostics in clinical bacteriology. Anal Bioanal Chem 2009; 394: 731-742.
9. Walsh TJ, Wissel MC, Grantham KJ, et al . Molecular diagnosis and species-specifi c identifi cation of medically important Aspergillus species by real time PCR in experimental invasive pulmonary Aspergillosis. J Clin Microbiol 2011; 49: 4150-4157.
10. Schabereiter-Gurtner C, Selitsch B, Rotter ML, Hirschl AM, Willinger B. Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens. J Clin Microbiol 2007; 45: 906-914.
11. Dunbar SA. Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid detection. Clin Chim Acta 2006; 363: 71-82.
12. Mahony J, Chong S, Merante F, et al . Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fl uid microbead-based assay. J Clin Microbio 2007; 45: 2965-2970.
13. Mazzulli T. Value of RVP in clinical settings: Intensive care. J Clin Virol 2007; 40 (Suppl. 1): S55-57.
14. Pabbaraju K, Wong S, Tokaryk KL, Fonseca K, Drews SJ. Comparison of the Luminex xTAG respiratory viral panel with xTAG respiratory viral panel fast for diagnosis of respiratory virus infections. J Clin Microbiol 2011; 49: 1738-1744.
15. Bortolin S, Black M, Modi H, et al. Analytical validation of the tag-it high-throughput microsphere-based universal array genotyping platform: Application to the multiplex detection of a panel of thrombophilia-associated single-nucleotide polymorphisms. Clin Chem 2004; 50: 2028-2036.
16. Babady NE, Miranda E, Gilhuley KA. Evaluation of Luminex xTAG® fungal analyte specifi c reagents for rapid identifi cation of clinically relevant fungi. J Clin Microbiol 2011; 49: 3777-3782.
17. Khot PD, Ko DL, Hackman RC, Fredricks DN. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fl uid. BMC Infect Dis 2008; 8: 73.