Molecular detection and species-specific identification of medically important Aspergillus species by real-time PCR in experimental invasive pulmonary aspergillosis

Journal of Clinical Microbiology

Volumn 49, Issue 12, 2011, Pages 4150-4157

  Walsh, Thomas J ^a,b,c   Wissel, Mark C ^d   Grantham, Kevin J ^d   Petraitiene, Ruta ^a,b,e   Petraitis, Vidmantas ^a,b,e   Kasai, Miki ^a   Francesconi, Andrea ^a   Cotton, Margaret P ^a   Hughes, Johanna E ^a   Greene, Lora ^a   Bacher, John D ^f   Manna, Pradip ^d,g   Salomoni, Martin ^d   Kleiboeker, Steven B ^d   Reddy, Sushruth K ^d  

^a NATIONAL CANCER INSTITUTE   (United States)

^b WEILL CORNELL MEDICAL COLLEGE   (United States)

^c NEW YORK PRESBYTERIAN HOSPITAL   (United States)

^d Viracor IBT Laboratories   (United States)

^e SAIC FREDERICK INC   (United States)

^f NATIONAL INSTITUTES OF HEALTH   (United States)

^g Physicians Reference Laboratory   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOTERICIN B DEOXYCHOLATE; GALACTOMANNAN; INTERNAL TRANSCRIBED SPACER 1;

ACCURACY; ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; ASPERGILLUS NIGER; ASPERGILLUS TERREUS; CONTROLLED STUDY; DIAGNOSTIC VALUE; DRUG DOSE COMPARISON; FEMALE; FUNGAL BURDEN; FUNGUS CULTURE; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; GALACTOMANNAN ASSAY; GENE AMPLIFICATION; GENE DOSAGE; IMMUNOCOMPROMISED PATIENT; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LUNG ASPERGILLOSIS; LUNG LAVAGE; LUNG PARENCHYMA; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGEN LOAD; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RABBIT; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SPECIES DIFFERENCE; SPECIES IDENTIFICATION; VALIDATION PROCESS;

ANIMALS; ASPERGILLUS; BACTERIAL LOAD; BRONCHOALVEOLAR LAVAGE FLUID; DISEASE MODELS, ANIMAL; DNA PRIMERS; DNA, RIBOSOMAL SPACER; FEMALE; INVASIVE PULMONARY ASPERGILLOSIS; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOLOGY; OLIGONUCLEOTIDE PROBES; RABBITS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 82455185117     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00570-11     Document Type: Article

References (30)

1. Balajee, S. A., et al. 2009. Molecular identification of Aspergillus species collected for the Transplant-Associated Infection Surveillance Network. J. Clin. Microbiol. 47:3138-3141.
2. Clancy, C. J., et al. 2007. Bronchoalveolar lavage galactomannan in diagnosis of invasive pulmonary aspergillosis among solid-organ transplant recipients. J. Clin. Microbiol. 45:1759-1765. (Pubitemid 46933903)
3. De Pauw, B., et al. 2008. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin. Infect. Dis. 46:1813-1821.
4. Francesconi, A., et al. 2006. Characterization and comparison of galactomannan enzyme immunoassay and quantitative real-time PCR assay for detection of Aspergillus fumigatus in bronchoalveolar lavage fluid from experimental invasive pulmonary aspergillosis. J. Clin. Microbiol. 44:2475-2480. (Pubitemid 44092414)
5. Francis, P., et al. 1994. Efficacy of unilamellar liposomal amphotericin B in treatment of pulmonary aspergillosis in persistently granulocytopenic rabbits: the potential role of bronchoalveolar D-mannitol and serum galactomannan as markers of infection. J. Infect. Dis. 169:356-368.
6. Hachem, R. Y., et al. 2004. Aspergillus terreus: an emerging amphotericin B-resistant opportunistic mold in patients with hematologic malignancies. Cancer 101:1594-1600. (Pubitemid 39318906)
7. Hahn, T., et al. 2002. Efficacy of high-efficiency particulate air filtration in preventing aspergillosis in immunocompromised patients with hematologic malignancies. Infect. Control Hosp. Epidemiol. 23:525-531. (Pubitemid 35033864)
8. Hayden, R., et al. 2008. Galactomannan antigenemia in pediatric oncology patients with invasive aspergillosis. Pediatr. Infect. Dis. J. 27:815-819.
9. Herrera, M. L., A. C. Vallor, J. A. Gelfond, T. F. Patterson, and B. L. Wickes. 2009. Strain-dependent variation in 18S ribosomal DNA copy numbers in Aspergillus fumigatus. J. Clin. Microbiol. 47:1325-1332.
10. Hope, W. W., et al. 2010. The initial 96 hours of invasive pulmonary aspergillosis: histopathology, comparative kinetics of galactomannan and (1→3)- β-D-glucan, and consequences of delayed antifungal therapy. Antimicrob. Agents Chemother. 54:4879-4886.
11. Hope, W. W., T. J. Walsh, and D. W. Denning. 2005. Laboratory diagnosis of invasive aspergillosis. Lancet Infect. Dis. 5:609-622. (Pubitemid 41383906)
12. Husain, S., et al. 2008. Performance characteristics of the platelia Aspergillus enzyme immunoassay for detection of Aspergillus galactomannan antigen in bronchoalveolar lavage fluid. Clin. Vaccine Immunol. 15:1760-1763.
13. Kahn, F. W., J. M. Jones, and D. M. England. 1986. The role of bronchoalveolar lavage in the diagnosis of invasive pulmonary aspergillosis. Am. J. Clin. Pathol. 86:518-523. (Pubitemid 16006882)
14. Kontoyiannis, D. P., et al. 2010. Prospective surveillance for invasive fungal infections in hematopoietic stem cell transplant recipients, 2001-2006: overview of the Transplant-Associated Infection Surveillance Network (TRANSNET) Database. Clin. Infect. Dis. 50:1091-1100.
15. Lass-Florl, C. 2010. In vitro susceptibility testing in Aspergillus species: an update. Future Microbiol. 5:789-799.
16. Luong, M. L., et al. 2011. Comparison of an Aspergillus real-time polymerase chain reaction assay with galactomannan testing of bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in lung transplant recipients. Clin. Infect. Dis. 52:1218-1226.
17. Marr, K. A., et al. 2004. Detection of galactomannan antigenemia by enzyme immunoassay for the diagnosis of invasive aspergillosis: variables that affect performance. J. Infect. Dis. 190:641-649. (Pubitemid 38982107)
18. Musher, B., et al. 2004. Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. J. Clin. Microbiol. 42:5517-5522. (Pubitemid 39664330)
19. Petraitiene, R., et al. 2001. Antifungal activity and pharmacokinetics of posaconazole (SCH 56592) in treatment and prevention of experimental invasive pulmonary aspergillosis: correlation with galactomannan antigenemia. Antimicrob. Agents Chemother. 45:857-869. (Pubitemid 32182037)
20. Petraitiene, R., et al. 2004. Efficacy, safety, and plasma pharmacokinetics of escalating dosages of intravenously administered ravuconazole lysine phosphoester for treatment of experimental pulmonary aspergillosis in persistently neutropenic rabbits. Antimicrob. Agents Chemother. 48:1188-1196. (Pubitemid 38405472)
21. Petraitis, V., et al. 2005. Efficacy and safety of generic amphotericin B in experimental pulmonary aspergillosis. Antimicrob. Agents Chemother. 49:1642-1645. (Pubitemid 40463411)
22. Saito, H., E. J. Anaissie, R. C. Morice, R. Dekmezian, and G. P. Bodey. 1988. Bronchoalveolar lavage in the diagnosis of pulmonary infiltrates in patients with acute leukemia. Chest 94:745-749.
23. Smith, N. L., and D. W. Denning. 2011. Underlying conditions in chronic pulmonary aspergillosis, including simple aspergilloma. Eur. Respir. J. 37:865-872.
24. Steinbach, W. J., et al. 2004. Infections due to Aspergillus terreus: a multicenter retrospective analysis of 83 cases. Clin. Infect. Dis. 39:192-198. (Pubitemid 38971489)
25. Vonberg, R. P., and P. Gastmeier. 2006. Nosocomial aspergillosis in outbreak settings. J. Hosp. Infect. 63:246-254.
26. Walsh, T. J., J. Bacher, and P. A. Pizzo. 1988. Chronic Silastic central venous catheterization for induction, maintenance and support of persistent granulocytopenia in rabbits. Lab. Anim. Sci. 38:467-471.
27. Walsh, T. J., and A. H. Groll. 2001. Overview: non-fumigatus species of Aspergillus: perspectives on emerging pathogens in immunocompromised hosts. Curr. Opin. Investig. Drugs 2:1366-1367. (Pubitemid 32994384)
28. Walsh, T. J., et al. 2003. Experimental pulmonary aspergillosis due to Aspergillus terreus: pathogenesis and treatment of an emerging fungal pathogen resistant to amphotericin B. J. Infect. Dis. 188:305-319. (Pubitemid 36898165)
29. Walsh, T. J., et al. 2009. Diagnostic imaging of experimental invasive pulmonary aspergillosis. Med. Mycol. 47(Suppl. 1):S138-S145.
30. Walsh, T. J., et al. 2004. Detection of galactomannan antigenemia in patients receiving piperacillin-tazobactam and correlations between in vitro, in vivo, and clinical properties of the drug-antigen interaction. J. Clin. Microbiol. 42:4744-4748. (Pubitemid 39362397)