Proteomic differential display identifies upregulated vinculin as a possible biomarker of pancreatic cancer

Oncology Reports

Volumn 28, Issue 5, 2012, Pages 1845-1850

  Wang, Yufeng ^a   Kuramitsu, Yasuhiro ^a   Ueno, Tomio ^a   Suzuki, Nobuaki ^a   Yoshino, Shigefumi ^a   Iizuka, Norio ^a   Zhang, Xiulian ^b   Akada, Junko ^a   Oka, Masaaki ^a   Nakamura, Kazuyuki ^a  

^a YAMAGUCHI UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b QINGDAO UNIVERSITY   (China)

Author keywords

Liquid chromatography tandem mass spectrometry; Pancreatic cancer; Two dimensional gel electrophoresis; Vinculin

Indexed keywords

ALPHA ENOLASE; CALRETICULIN; GLUTATHIONE SYNTHASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; STATHMIN; VINCULIN;

ADULT; AGED; ARTICLE; CELL ADHESION; CELL MIGRATION; CLINICAL ARTICLE; CONTROLLED STUDY; DIFFERENTIAL DISPLAY; DNA SEQUENCE; FEMALE; HUMAN; HUMAN TISSUE; LIQUID CHROMATOGRAPHY; MALE; NUCLEOTIDE SEQUENCE; OUTCOME ASSESSMENT; PANCREAS CANCER; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEOMICS; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY; TISSUE CHARACTERIZATION; TWO DIMENSIONAL GEL ELECTROPHORESIS; UPREGULATION; WESTERN BLOTTING;

AGED; CALRETICULIN; FEMALE; GENE EXPRESSION PROFILING; GLUTATHIONE SYNTHASE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; MALE; MIDDLE AGED; PANCREATIC NEOPLASMS; PHOSPHOPYRUVATE HYDRATASE; PROTEOMICS; STATHMIN; TUMOR MARKERS, BIOLOGICAL; UP-REGULATION; VINCULIN;

EID: 84866437004     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2012.2004     Document Type: Article

References (22)

1. Jemal A, Siegel R, Ward E, Hao Y, Xu J and Thun MJ: Cancer statistics, 2009. CA Cancer J Clin 59: 225-249, 2009.
2. Kuramitsu Y, Miyamoto H, Tanaka T, Zhang X, Fujimoto M, Ueda K, Tanaka T, Hamano K and Nakamura K: Proteomic differential display analysis identified upregulated astrocytic phosphoprotein PEA-15 in human malignant pleural mesothelioma cell lines. Proteomics 9: 5078-5089, 2009.
3. Luk JM, Lam CT, Siu AF, Lam BY, Ng IO, Hu MY, Che CM and Fan ST: Proteomic profiling of hepatocellular carcinoma in Chinese cohort reveals heat-shock proteins (Hsp27, Hsp70, GRP78) up-regulation and their associated prognostic values. Proteomics 6: 1049-1057, 2006. (Pubitemid 43260229)
4. Roth U, Razawi H, Hommer J, Engelmann K, Schwientek T, Müller S, Baldus SE, Patsos G, Corfield AP, Paraskeva C and Hanisch FG: Differential expression proteomics of human colorectal cancer based on a syngeneic cellular model for the progression of adenoma to carcinoma. Proteomics 10: 194-202, 2010.
5. Kuramitsu Y, Harada T, Takashima M, Yokoyama Y, Hidaka I, Iizuka N, Toda T, Fujimoto M, Zhang X, Sakaida I, et al: Increased expression and phosphorylation of liver glutamine synthetase in well-differentiated hepatocellular carcinoma tissues from patients infected with hepatitis C virus. Electrophoresis 27: 1651-1658, 2006.
6. Tanaka T, Kuramitsu Y, Fujimoto M, Naito S, Oka M and Nakamura K: Downregulation of two isoforms of ubiquitin carboxyl-terminal hydrolase isozyme L1 correlates with high metastatic potentials of human SN12C renal cell carcinoma cell clones. Electrophoresis 29: 2651-2659, 2008. (Pubitemid 351911893)
7. Kuramitsu Y, Hayashi E, Okada F, Tanaka T, Zhang X, Ueyama Y and Nakamura K: Proteomic analysis for nuclear proteins related to tumour malignant progression: a comparative proteomic study between malignant progressive cells and regressive cells. Anticancer Res 30: 2093-2099, 2010.
8. Kuramitsu Y, Baron B, Yoshino S, Zhang X, Tanaka T, Yashiro M, Hirakawa K, Oka M and Nakamura K: Proteomic differential display analysis shows up-regulation of 14-3-3 protein sigma in human scirrhous-type gastric carcinoma cells. Anticancer Res 30: 4459-4465, 2010.
9. Kuramitsu Y, Taba K, Ryozawa S, Yoshida K, Zhang X, Tanaka T, Maehara S, Maehara Y, Sakaida I and Nakamura K: Identification of up- and down-regulated proteins in gemcitabine-resistant pancreatic cancer cells using two-dimensional gel electrophoresis and mass spectrometry. Anticancer Res 30: 3367-3372, 2010.
10. Kuramitsu Y, Hayashi E, Okada F, Zhang X, Tanaka T, Ueyama Y and Nakamura K: Staining with highly sensitive Coomassie brilliant blue SeePico™ stain after Flamingo™ fluorescent gel stain is useful for cancer proteomic analysis by means of two-dimensional gel electrophoresis. Anticancer Res 30: 4001-4005, 2010.
11. Mori-Iwamoto S, Kuramitsu Y, Ryozawa S, Mikuria K, Fujimoto M, Maehara S, Maehara Y, Okita K, Nakamura K and Sakaida I: Proteomics finding heat shock protein 27 as a biomarker for resistance of pancreatic cancer cells to gemcitabine. Int J Oncol 31: 1345-1350, 2007.
12. Volberg T, Geiger B, Kam Z, Pankov R, Simcha I, Sabanay H, Coll JL, Adamson E and Ben-Ze'ev A: Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption. J Cell Sci 108: 2253-2260, 1995.
13. Xu W, Baribault H and Adamson ED: Vinculin knockout results in heart and brain defects during embryonic development. Development 125: 327-337, 1998. (Pubitemid 28064445)
14. Mikuriya K, Kuramitsu Y, Ryozawa S, Fujimoto M, Mori S, Oka M, Hamano K, Okita K, Sakaida I and Nakamura K: Expression of glycolytic enzymes is increased in pancreatic cancerous tissues as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry. Int J Oncol 30: 849-855, 2007.
15. Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW and Liddington RC: Structural basis for vinculin activation at sites of cell adhesion. Nature 430: 583-586, 2004. (Pubitemid 38998634)
16. Rüdiger M: Vinculin and alpha-catenin: shared and unique functions in adherens junctions. Bioessays 20: 733-740, 1998.
17. Rodríguez Fernández JL, Geiger B, Salomon D, Sabanay I, Zöller M and Ben-Ze'ev A: Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA. J Cell Biol 119: 427-438, 1992.
18. Subauste MC, Pertz O, Adamson ED, Turner CE, Junger S and Hahn KM: Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. J Cell Biol 165: 371-381, 2004. (Pubitemid 38649912)
19. Frisch SM and Francis H: Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol 124: 619-626, 1994. (Pubitemid 24064472)
20. Ruiz C, Holz DR, Oeggerli M, Schneider S, Gonzales IM, Kiefer JM, Zellweger T, Bachmann A, Koivisto PA, Helin HJ, et al: Amplification and overexpression of vinculin are associated with increased tumour cell proliferation and progression in advanced prostate cancer. J Pathol 223: 543-552, 2011.
21. Thorsen K, Sørensen KD, Brems-Eskildsen AS, Modin C, Gaustadnes M, Hein AM, Kruhøffer M, Laurberg S, Borre M, Wang K, et al: Alternative splicing in colon, bladder, and prostate cancer identified by exon array analysis. Mol Cell Proteomics 7: 1214-1224, 2008. (Pubitemid 352038268)
22. Ziegler WH, Liddington RC and Critchley DR: The structure and regulation of vinculin. Trends Cell Biol 16: 453-460, 2006. (Pubitemid 44293400)