The Escherichia coli GTPase ObgE modulates hydroxyl radical levels in response to DNA replication fork arrest

FEBS Journal

Volumn 279, Issue 19, 2012, Pages 3692-3704

  Kint, Cyrielle I ^a   Verstraeten, Natalie ^a   Wens, Inez ^a   Liebens, Veerle R ^a   Hofkens, Johan ^a   Versées, Wim ^b,c   Fauvart, Maarten ^a   Michiels, Jan ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^c DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

GTPase; hydroxyurea; ObgE; random mutagenesis; toxin antitoxin module

Indexed keywords

ANTITOXIN; BACTERIAL ENZYME; GUANOSINE TRIPHOSPHATASE; HYDROXYL RADICAL; HYDROXYUREA; MAZEF; PROTEIN OBGE; PROTEIN TONB; RELBE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHROMOSOME DELETION; CONTROLLED STUDY; DRUG SENSITIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE INSERTION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MOTIF; RNA REPLICATION; TRANSPOSON;

AMINO ACID SEQUENCE; CHROMOSOMES, BACTERIAL; DNA REPLICATION; DRUG HYPERSENSITIVITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FREE RADICAL SCAVENGERS; GENE DELETION; HYDROXYL RADICAL; HYDROXYUREA; MOLECULAR SEQUENCE DATA; MONOMERIC GTP-BINDING PROTEINS; MUTAGENESIS; MUTATION; NUCLEIC ACID SYNTHESIS INHIBITORS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID;

ESCHERICHIA COLI;

EID: 84866369581     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2012.08731.x     Document Type: Article

References (34)

1. Kobayashi G, Moriya S, &, Wada C, (2001) Deficiency of essential GTP-binding protein ObgE in Escherichia coli inhibits chromosome partition. Mol Microbiol 41, 1037-1051. (Pubitemid 36124701)
2. Sato A, Kobayashi G, Hayashi H, Yoshida H, Wada A, Maeda M, Hiraga S, Takeyasu K, &, Wada C, (2005) The GTP binding protein Obg homolog ObgE is involved in ribosome maturation. Genes Cells 10, 393-408. (Pubitemid 40590358)
3. Verstraeten N, Fauvart M, Versees W, &, Michiels J, (2011) The universally conserved prokaryotic GTPases. Microbiol Mol Biol Rev 75, 507-542.
4. B. J Bacteriol 181, 4653-4660. (Pubitemid 29357949)
5. Raskin DM, Judson N, &, Mekalanos JJ, (2007) Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Proc Natl Acad Sci USA 104, 4636-4641. (Pubitemid 47186277)
6. Kuo S, Demeler B, &, Haldenwang WG, (2008) The growth-promoting and stress response activities of the Bacillus subtilis GTP binding protein Obg are separable by mutation. J Bacteriol 190, 6625-6635.
7. Vidwans SJ, Ireton K, &, Grossman AD, (1995) Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis. J Bacteriol 177, 3308-3311.
8. Kok J, Trach KA, &, Hoch JA, (1994) Effects on Bacillus subtilis of a conditional lethal mutation in the essential GTP-binding protein Obg. J Bacteriol 176, 7155-7160. (Pubitemid 24364040)
9. Okamoto S, &, Ochi K, (1998) An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor. Mol Microbiol 30, 107-119. (Pubitemid 28446624)
10. Foti JJ, Persky NS, Ferullo DJ, &, Lovett ST, (2007) Chromosome segregation control by Escherichia coli ObgE GTPase. Mol Microbiol 65, 569-581. (Pubitemid 47052736)
11. Foti JJ, Schienda J, Sutera VA Jr, &, Lovett ST, (2005) A bacterial G protein-mediated response to replication arrest. Mol Cell 17, 549-560. (Pubitemid 40269119)
12. Shah S, Das B, &, Bhadra RK, (2008) Functional analysis of the essential GTP-binding-protein-coding gene cgtA of Vibrio cholerae. J Bacteriol 190, 4764-4771. (Pubitemid 351898995)
13. Davies BW, Kohanski MA, Simmons LA, Winkler JA, Collins JJ, &, Walker GC, (2009) Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli. Mol Cell 36, 845-860.
14. Buglino J, Shen V, Hakimian P, &, Lima CD, (2002) Structural and biochemical analysis of the Obg GTP binding protein. Structure 10, 1581-1592. (Pubitemid 35351498)
15. Lin B, &, Maddock JR, (2001) The N-terminal domain of the Caulobacter crescentus CgtA protein does not function as a guanine nucleotide exchange factor. FEBS Lett 489, 108-111.
16. Bourne HR, Sanders DA, &, McCormick F, (1991) The GTPase superfamily: conserved structure and molecular mechanism. Nature 349, 117-127. (Pubitemid 21912023)
17. Polkinghorne A, &, Vaughan L, (2011) Chlamydia abortus YhbZ, a truncated Obg family GTPase, associates with the Escherichia coli large ribosomal subunit. Microb Pathog 50, 200-206.
18. C is required for progression through the cell cycle and for maintaining 50S ribosomal subunit levels. Mol Microbiol 54, 1379-1392. (Pubitemid 39578279)
19. Lapik YR, Misra JM, Lau LF, &, Pestov DG, (2007) Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1. Mol Cell Biol 27, 7735-7744. (Pubitemid 350033678)
20. Imlay JA, (2008) Cellular defenses against superoxide and hydrogen peroxide. Annu Rev Biochem 77, 755-776.
21. C protein cosediments with the free 50S ribosomal subunit. J Bacteriol 186, 481-489. (Pubitemid 38076439)
22. Kukimoto-Niino M, Murayama K, Inoue M, Terada T, Tame JR, Kuramitsu S, Shirouzu M, &, Yokoyama S, (2004) Crystal structure of the GTP-binding protein Obg from Thermus thermophilus HB8. J Mol Biol 337, 761-770. (Pubitemid 38326889)
23. Persky NS, Ferullo DJ, Cooper DL, Moore HR, &, Lovett ST, (2009) The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli. Mol Microbiol 73, 253-266.
24. E cofractionates with the 50S ribosomal subunit and interacts with SpoT, a ppGpp synthetase/hydrolase. J Bacteriol 186, 5249-5257. (Pubitemid 39038128)
25. Foti JJ, Devadoss B, Winkler JA, Collins JJ, &, Walker GC, (2012) Oxidation of the guanine nucleotide pool underlies cell death by bactericidal antibiotics. Science 336, 315-319.
26. D'Hooghe I, Michiels J, Vlassak K, Verreth C, Waelkens F, &, Vanderleyden J, (1995) Structural and functional analysis of the fixLJ genes of Rhizobium leguminosarum biovar phaseoli CNPAF512. Mol Gen Genet 249, 117-126.
27. Kuong KJ, &, Kuzminov A, (2009) Cyanide, peroxide and nitric oxide formation in solutions of hydroxyurea causes cellular toxicity and may contribute to its therapeutic potency. J Mol Biol 390, 845-862.
28. Datsenko KA, &, Wanner BL, (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97, 6640-6645. (Pubitemid 30412767)
29. Michiels J, Vande BroekA, &, Vanderleyden J, (1991) Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recA gene. Mol Gen Genet 228, 486-490.
30. Patrick WM, Firth AE, &, Blackburn JM, (2003) User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries. Protein Eng 16, 451-457. (Pubitemid 36917359)
31. Harlow E, &, Lane D, (1998) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
32. Kim DE, Chivian D, &, Baker D, (2004) Protein structure prediction and analysis using the Robetta server. Nucleic Acids Res 32, W526-W531. (Pubitemid 38997389)
33. Kim DE, Chivian D, Malmstrom L, &, Baker D, (2005) Automated prediction of domain boundaries in CASP6 targets using Ginzu and RosettaDOM. Proteins 61 (Suppl. 7), 193-200. (Pubitemid 43069974)
34. Raman S, Vernon R, Thompson J, Tyka M, Sadreyev R, Pei J, Kim D, Kellogg E, DiMaio F, Lange O, et al. (2009) Structure prediction for CASP8 with all-atom refinement using Rosetta. Proteins 77 (Suppl. 9), 89-99.