Manipulation of 3D cluster size and geometry by release from 2D micropatterns

Cellular and Molecular Bioengineering

Volumn 5, Issue 3, 2012, Pages 299-306

  Leight, Jennifer L ^a   Liu, Wendy F ^b   Chaturvedi, Ritika R ^a   Chen, Sophia ^a   Yang, Michael T ^a   Raghavan, Srivatsan ^b   Chen, Christopher S ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

2D; 3D; Collagenase; Micropatterning; Multicellular

Indexed keywords

COLLAGEN; COLLAGENASE;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL CULTURE; CELL PROLIFERATION; CELL STRUCTURE; CELLULAR PARAMETERS; COLLAGEN DEGRADATION; GEOMETRY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RAT; THREE DIMENSIONAL CELL CLUSTER SIZE; TWO DIMENSIONAL MICROPATTERN;

EID: 84866363835     PISSN: 18655025     EISSN: 18655033     Source Type: Journal    
DOI: 10.1007/s12195-012-0236-9     Document Type: Article

References (22)

1. Bhatia, S. N., M. L. Yarmush, and M. Toner. Controlling cell interactions by micropatterning in co-cultures: hepatocytes and 3T3 fibroblasts. J. Biomed. Mater. Res. 34(2): 189-199, 1997.
2. Burridge, P. W., et al. Improved human embryonic stem cell embryoid body homogeneity and cardiomyocyte differentiation from a novel V-96 plate aggregation system highlights interline variability. Stem Cells 25(4):929-938, 2007.
3. Casciari, J. J., S. V. Sotirchos, and R. M. Sutherland. Glucose diffusivity in multicellular tumor spheroids. Cancer Res. 48(14):3905-3909, 1988.
4. Fukuda, J., and K. Nakazawa. Orderly arrangement of hepatocyte spheroids on a microfabricated chip. Tissue Eng. 11(7-8):1254-1262, 2005.
5. Fukuda, J., et al. Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components. Biomaterials 27(8):1479-1486, 2006.
6. Kaji, H., et al. Engineering systems for the generation of patterned co-cultures for controlling cell-cell interactions. Biochim. Biophys. Acta 1810(3):239-250, 2011.
7. Kale, S., et al. Three-dimensional cellular development is essential for ex vivo formation of human bone. Nat. Biotechnol. 18(9):954-958, 2000.
8. Kelm, J. M., et al. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol. Bioeng. 83(2):173-180, 2003.
9. Korff, T., and H. G. Augustin. Integration of endothelial cells in multicellular spheroids prevents apoptosis and induces differentiation. J. Cell Biol. 143(5):1341-1352, 1998.
10. Kunz-Schughart, L. A., et al. Potential of fibroblasts to regulate the formation of three-dimensional vessel-like structures from endothelial cells in vitro. Am. J. Physiol. Cell Physiol. 290(5):C1385-C1398, 2006.
11. Landry, J., et al. Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J. Cell Biol. 101(3): 914-923, 1985.
12. Liu, W. F., et al. E-cadherin engagement stimulates proliferation via Rac1. J. Cell Biol. 173(3):431-441, 2006.
13. Mohr, J. C., J. J. de Pablo, and S. P. Palecek. 3-D microwell culture of human embryonic stem cells. Biomaterials 27(36):6032-6042, 2006.
14. Perez-Pomares, J. M., et al. In vitro self-assembly of proepicardial cell aggregates: an embryonic vasculogenic model for vascular tissue engineering. Anat. Rec. A Discov. Mol. Cell Evol. Biol. 288(7):700-713, 2006.
15. Reynolds, B. A., and S. Weiss. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255(5052):1707-1710, 1992.
16. Sutherland, R. M. Cell and environment interactions in tumor microregions: the multicell spheroid model. Science 240(4849):177-184, 1988.
17. Tan, J. L., et al. Simple approach to micropattern cells on common culture substrates by tuning substrate wettability. Tissue Eng. 10(5-6):865-872, 2004.
18. Tien, J., C. M. Nelson, and C. S. Chen. Fabrication of aligned microstructures with a single elastomeric stamp. Proc. Natl Acad. Sci. USA. 99(4):1758-1762, 2002.
19. Tsuda, Y., et al. The use of patterned dual thermoresponsive surfaces for the collective recovery as co-cultured cell sheets. Biomaterials 26(14):1885-1893, 2005.
20. Ungrin, M. D., et al. Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One 3(2):e1565, 2008.
21. Wang, W., et al. 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials 30(14): 2705-2715, 2009.
22. Yeo, W. S., and M. Mrksich. Electroactive self-assembled monolayers that permit orthogonal control over the adhesion of cells to patterned substrates. Langmuir 22(25):10816-10820, 2006.