Single-step purification and characterization of recombinant aspartase of aeromonas media NFB-5

Applied Biochemistry and Biotechnology

Volumn 167, Issue 5, 2012, Pages 991-1001

  Singh, Ram Sarup ^a   Yadav, Mukesh ^a  

^a PUNJABI UNIVERSITY   (India)

Author keywords

Aeromonas media; Amino acid sequence; Aspartase; Characterization; Purification; Thermodynamics

Indexed keywords

AEROMONAS; AMINO ACID SEQUENCE; ASPARTASE; CLONED GENES; CODING GENES; DIVALENT METAL ION; EXPRESSION VECTORS; FUMARIC ACIDS; HIS-TAG; L-ASPARTIC ACID; MAGNESIUM IONS; OPTIMAL ACTIVITY; OPTIMUM TEMPERATURE; PH RANGE; SDS-PAGE; SINGLE-STEP PURIFICATION;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CHARACTERIZATION; CLONING; ENZYMES; ESCHERICHIA COLI; METAL IONS; MOLECULAR WEIGHT; PURIFICATION; THERMODYNAMICS;

GENE EXPRESSION;

AMINO ACID; ASPARTATE AMMONIA LYASE; CALCIUM ION; MAGNESIUM ION; MANGANESE; METAL ION; NICKEL; NITRILOTRIACETIC ACID; POLYHISTIDINE TAG; POTASSIUM DIHYDROGEN PHOSPHATE; RECOMBINANT ASPARTASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

AEROMONAS; AEROMONAS MEDIA; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; CHEMICAL ANALYSIS; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; ROOM TEMPERATURE; THERMODYNAMICS;

AEROMONAS; AMINO ACID SEQUENCE; ASPARTATE AMMONIA-LYASE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENTROPY; ENZYME ACTIVATION; ENZYME STABILITY; HALF-LIFE; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; TEMPERATURE;

AEROMONAS MEDIA; ESCHERICHIA COLI;

EID: 84866363102     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9589-8     Document Type: Conference Paper

References (22)

1. Papierz, M., Grazyna, G., Sobierajski, B., & Chmiel, A. (2007). Polish Journal of Microbiology, 56, 71-76.
2. Quastel, J. H., & Woolf, B. (1926). Journal of Biochemistry, 20, 545-555.
3. Kisumi, M., Ashikaga, Y., & Chibata, I. (1960). Bulletin of Agricultural Chemistry Japan, 24, 296-305.
4. Takagi, J. S., Ida, N., Tokushige, M., Sakamoto, H., & Shimura, Y. (1985). Nucleic Acids Research, 13, 2063-2074.
5. Kazuoka, T., Masuda, Y., Oikawa, T., & Soda, K. (2003). Journal of Biochemistry, 133, 51-56.
6. Takagi, J. S., Tokushige, M., & Shimura, Y. (1986). Journal of Biochemistry, 100, 697-705.
7. Kawata, Y., Tamura, K., Kawamura, M., Ikei, K., Mizobata, T., Nagai, J., et al. (2000). European Journal of Biochemistry, 267, 1847-1857.
8. Sun, D. X., & Setlow, P. (1991). Journal of Bacteriology, 173, 3831-3845.
9. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265-275.
10. Tokushige, M. (1985). Methods in Enzymology, 113, 618-627.
11. Laemmli, U. K. (1970). Nature, 227, 680-685.
12. Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D., et al. (2005). In J. M. Walker (Ed.), The Proteomics Protocols Handbook (pp. 571-607). USA: Humana Press.
13. Pal, A., & Khanum, F. (2010). Journal of Biochemistry and Technology, 2, 203-209.
14. Jayasekera, M. M., Shi, W., Farber, G. K., & Viola, R. E. (1997). Biochemistry, 36, 9145-9150.
15. Shi, W., Dunbar, J., Jayasekera, M. M., Viola, R. E., & Farber, G. K. (1997). Biochemistry, 36, 9136-9144.
16. Asano, Y., Kira, I., & Yokozeki, K. (2005). Biomolecular Engineering, 22, 95-101.
17. Veetil, V. P., Raj, H., Quax, W. J., Janssen, D. B., & Poelarends, G. J. (2009). FEBS Journal, 276, 2994-3007.
18. Komatsubara, S., Taniguchi, T., & Kisumi, M. (1986). Journal of Biotechnology, 3, 281-291.
19. Williams, V. R., & Lartigue, D. J. (1967). Journal of Biological Chemistry, 242, 2973-2978.
20. Suzuki, S., Yamagushi, J., & Togushige, M. (1973). Biochimica et Biophysica Acta, 321, 369-381.
21. Zhang, H. Y., Zhang, J., Lin, L., Du, W. Y., & Lu, J. (1993). Biochemical and Biophysical Research Communications, 192, 15-21.
22. Marangoni, A. G. (2003). Enzyme Kinetics: A Modern Approach. Hoboken: Wiley.