Rhamnosyltransferase genes migA and wapR are regulated in a differential manner to modulate the quantities of core oligosaccharide glycoforms produced by Pseudomonas aeruginosa

Journal of Bacteriology

Volumn 194, Issue 16, 2012, Pages 4295-4300

  Kocíncová, Dana ^a   Ostler, Sarah L ^a   Anderson, Erin M ^a   Lam, Joseph S ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MIGA PROTEIN; OLIGOSACCHARIDE; POLYSACCHARIDE; UNCLASSIFIED DRUG; WAPR PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; CORRELATION ANALYSIS; GENE CONTROL; GENE EXPRESSION; GENETIC ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS AERUGINOSA;

EID: 84866342421     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.05741-11     Document Type: Article

References (21)

1. Brint JM, Ohman DE. 1995. Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J. Bacteriol. 177:7155-7163.
2. Brock JE, Pourshahian S, Giliberti J, Limbach PA, Janssen GR. 2008. Ribosomes bind leaderless mRNA in Escherichia coli through recognition of their 5'-terminal AUG. RNA 14:2159-2169.
3. de Kievit TR, Dasgupta T, Schweizer H, Lam JS. 1995. Molecular cloning and characterization of the rfc gene of Pseudomonas aeruginosa (serotype O5). Mol. Microbiol. 16:565-574.
4. de Kievit TR, Lam JS. 1994. Monoclonal antibodies that distinguish inner core, outer core, and lipid A regions of Pseudomonas aeruginosa lipopolysaccharide. J. Bacteriol. 176:7129-7139.
5. Dotsch A, et al. 2012. The Pseudomonas aeruginosa transcriptome in planktonic cultures and static biofilms using RNA sequencing. PLoS One 7:e31092. doi:10.1371/journal.pone.0031092.
6. Gruber TM, Gross CA. 2003. Multiple sigma subunits and the partitioning of bacterial transcription space. Annu. Rev. Microbiol. 57:441-466.
7. Hancock RE, Carey AM. 1979. Outer membrane of Pseudomonas aeruginosa: heat-2-mercaptoethanol-modifiable proteins. J. Bacteriol. 140:902-910.
8. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:77-86.
9. Hoang TT, Kutchma AJ, Becher A, Schweizer HP. 2000. Integrationproficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains. Plasmid 43:59-72.
10. King JD, Kocíncová D, Westman EL, Lam JS. 2009. Review: lipopolysaccharide biosynthesis in Pseudomonas aeruginosa. Innate Immun. 15:261-312.
11. Kocincova D, Hao Y, Vinogradov E, Lam JS. 2011. Evidence that WapB is a 1,2-glucosyltransferase of Pseudomonas aeruginosa involved in lipopolysaccharide outer core biosynthesis. J. Bacteriol. 193:2708-2716.
12. Poon KK, Westman EL, Vinogradov E, Jin S, Lam JS. 2008. Functional characterization of MigA and WapR: putative rhamnosyltransferases involved in outer core oligosaccharide biosynthesis of Pseudomonas aeruginosa. J. Bacteriol. 190:1857-1865.
13. Potvin E, Sanschagrin F, Levesque RC. 2008. Sigma factors in Pseudomonas aeruginosa. FEMS Microbiol. Rev. 32:38-55.
14. Rust L, Pesci EC, Iglewski BH. 1996. Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region. J. Bacteriol. 178:1134-1140.
15. Savli H, et al. 2003. Expression stability of six housekeeping genes: a proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR. J. Med. Microbiol. 52:403-408.
16. Schweizer HP, Chuanchuen R. 2001. Small broad-host-range lacZ operon fusion vector with low background activity. Biotechniques 31:1260-1262.
17. Simon R, Priefer U, Puhler A. 1983. A broad host range mobilization system for in vivo genetic-engineering-transposon mutagenesis in gramnegative bacteria. Biotechnology 1:784-791.
18. Sinoquet C, Demey S, Braun F. 2008. Large-scale computational and statistical analyses of high transcription potentialities in 32 prokaryotic genomes. Nucleic Acids Res. 36:3332-3340.
19. Wang J, Lory S, Ramphal R, Jin S. 1996. Isolation and characterization of Pseudomonas aeruginosa genes inducible by respiratory mucus derived from cystic fibrosis patients. Mol. Microbiol. 22:1005-1012.
20. West SE, Schweizer HP, Dall C, Sample AK, Runyen-Janecky LJ. 1994. Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 148:81-86.
21. Yang H, et al. 2000. migA, a quorum-responsive gene of Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-mass lipopolysaccharide. Microbiology 146:2509-2519.