A practical random mutagenesis system for probiotic Lactobacillus casei using Tn5 transposition complexes

Journal of Applied Microbiology

Volumn 109, Issue 2, 2010, Pages 657-666

  Ito, M ^a   Kim, Y G ^a   Tsuji, H ^b   Kiwaki, M ^b   Nomoto, K ^b   Tanaka, R ^b   Okada, N ^a   Danbara, H ^a  

^a KITASATO UNIVERSITY   (Japan)

^b YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

Author keywords

auxotrophic requirement; electroporation; EZ::TN transposome; Lactobacillus casei; probiotics; random mutagenesis; Tn5 transposon

Indexed keywords

GENES;

AUXOTROPHIC REQUIREMENT; BENEFICIAL EFFECTS; ELECTROPORATION; EZ::TN TRANSPOSOME; INSERTION MUTANTS; LACTOBACILLUS CASEI; PROBIOTIC LACTOBACILLI; PROBIOTICS; RANDOM MUTAGENESIS; TN5 TRANSPOSON;

MUTAGENESIS;

PLASMID DNA; PROBIOTIC AGENT;

BACTERIUM; GENOME; MUTATION; NUTRITIONAL REQUIREMENT; PLASMID; PROBIOTICS;

ARTICLE; AUXOTROPHIC MUTANT; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATE; BACTERIUM MUTANT; COLONY FORMING UNIT; DNA SEQUENCE; ELECTROPORATION; GENE INSERTION; GENE LIBRARY; GENETIC SCREENING; INVERSE POLYMERASE CHAIN REACTION; LACTOBACILLUS CASEI; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROCESS OPTIMIZATION; TRANSPOSON;

BACTERIA (MICROORGANISMS); LACTOBACILLUS CASEI;

EID: 77954518675     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04690.x     Document Type: Article

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