Gene cloning, purification, and characterization of a novel peptidoglutaminase-asparaginase from Aspergillus sojae

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5182-5188

  Ito, Kotaro ^a,b   Matsushima, Kenichiro ^a   Koyama, Yasuji ^b  

^a KIKKOMAN CORPORATION   (Japan)

^b Noda Institute for Scientific Research   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINYL; ASPERGILLUS ORYZAE; CELL SURFACES; DEAMIDATED; EXPRESSED SEQUENCE TAGS; FERMENTED FOODS; GEL-FILTRATION CHROMATOGRAPHY; GENE CLONING; GENOME SEQUENCES; GLUTAMINASE; GLUTAMINYL; L-GLUTAMATE; L-GLUTAMINE; NATIVE FORMS; OPTIMAL TEMPERATURE; OVER-EXPRESSION; SALT TOLERANT; SDS-PAGE; SODIUM PHOSPHATE BUFFER; STRUCTURAL GENE; SUBMERGED CULTURES; SUBSTRATE SPECIFICITY; UMAMI TASTE;

AMINO ACIDS; ASPERGILLUS; CELL MEMBRANES; CLONING; DIMERS; ENZYMES; GEL PERMEATION CHROMATOGRAPHY; OPTIMIZATION; PURIFICATION; SODIUM COMPOUNDS;

GENE ENCODING;

AMIDASE; ASPARAGINASE; PEPTIDYL GLUTAMINASE; PEPTIDYL-GLUTAMINASE; PRIMER DNA;

ENZYME ACTIVITY; FERMENTATION; FOOD PRODUCTION; FUNGUS; GENE EXPRESSION; GENOME; HYDROLYSIS; PH; SALINITY TOLERANCE;

ARTICLE; ASPERGILLUS; DNA SEQUENCE; ENZYMOLOGY; EXPRESSED SEQUENCE TAG; GEL CHROMATOGRAPHY; GENE STRUCTURE; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; TEMPERATURE;

AMIDOHYDROLASES; ASPARAGINASE; ASPERGILLUS; BASE SEQUENCE; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXPRESSED SEQUENCE TAGS; GENE COMPONENTS; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

ASPERGILLUS ORYZAE; ASPERGILLUS SOJAE; CRYPTOCOCCUS FLAVESCENS;

EID: 84866171039     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00765-12     Document Type: Article

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