Expression of cellobiose dehydrogenase from Neurospora crassa in Pichia pastoris and its purification and characterization

Protein Expression and Purification

Volumn 75, Issue 1, 2011, Pages 63-69

  Zhang, Ruifu ^a   Fan, Zhiliang ^a   Kasuga, Takao ^b,c  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Cellobiose dehydrogenase; Characterization; Heterologous expression; Neurospora crassa; Pichia pastoris

Indexed keywords

CELLOBIOSE QUINONE OXIDOREDUCTASE; CELLOBIOSE-QUINONE OXIDOREDUCTASE; OXIDOREDUCTASE; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; FUNGAL GENE; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NEUROSPORA CRASSA; PH; PICHIA; TEMPERATURE;

CARBOHYDRATE DEHYDROGENASES; CLONING, MOLECULAR; ENZYME STABILITY; GENE EXPRESSION; GENES, FUNGAL; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; KINETICS; NEUROSPORA CRASSA; PICHIA; RECOMBINANT PROTEINS; TEMPERATURE;

NEUROSPORA CRASSA; PICHIA PASTORIS;

EID: 78049264739     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.08.003     Document Type: Article

References (41)

1. B.P. Roy, T. Dumonceaux, A.A. Koukoulas, and F.S. Archibald Purification and characterization of cellobiose dehydrogenases from the white rot fungus Trametes versicolor Appl. Environ. Microbiol. 62 1996 4417 4427
2. B. Li, S.R. Nagalla, and V. Renganathan Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium Appl. Environ. Microbiol. 62 1996 1329 1335
3. C. Schou, M.H. Christensen, and M. Schulein Characterization of a cellobiose dehydrogenase from Humicola insolens Biochem. J. 330 Pt 1 1998 565 571
4. K.N. Karapetyan, T.V. Fedorova, L.G. Vasil'chenko, R. Ludwig, D. Haltrich, and M.L. Rabinovich Properties of neutral cellobiose dehydrogenase from the ascomycete Chaetomium sp. INBI 2-26(-) and comparison with basidiomycetous cellobiose dehydrogenases J. Biotechnol. 121 2006 34 48
5. D.R. Schmidhalter, and G. Canevascini Isolation and characterization of the cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana (Schum ex Fr.) Karst Arch. Biochem. Biophys. 300 1993 559 563 (Pubitemid 23232911)
6. U. Baminger, S.S. Subramaniam, V. Renganathan, and D. Haltrich Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii Appl. Environ. Microbiol. 67 2001 1766 1774
7. G. Henriksson, G. Johansson, and G. Pettersson A critical review of cellobiose dehydrogenases J. Biotechnol. 78 2000 93 113
8. M. Zamocky, M. Hallberg, R. Ludwig, C. Divne, and D. Haltrich Ancestral gene fusion in cellobiose dehydrogenases reflects a specific evolution of GMC oxidoreductases in fungi Gene 338 2004 1 14
9. B.M. Hallberg, G. Henriksson, G. Pettersson, and C. Divne Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase J. Mol. Biol. 315 2002 421 434
10. B.M. Hallberg, T. Bergfors, K. Backbro, G. Pettersson, G. Henriksson, and C. Divne A new scaffold for binding haem in the cytochrome domain of the extracellular flavocytochrome cellobiose dehydrogenase Structure 8 2000 79 88
11. P.C. Stapleton, M.M. O'Brien, J. O'Callaghan, and A.D. Dobson Molecular cloning of the cellobiose dehydrogenase gene from Trametes versicolor and expression in Pichia pastoris Enzyme Microb. Technol. 34 2004 55 63
12. M. Yoshida, K. Igarashi, R. Kawai, K. Aida, and M. Samejima Differential transcription of beta-glucosidase and cellobiose dehydrogenase genes in cellulose degradation by the basidiomycete Phanerochaete chrysosporium FEMS Microbiol. Lett. 235 2004 177 182
13. M.D. Cameron, and S.D. Aust Cellobiose dehydrogenase - an extracellular fungal flavocytochrome Enzyme Microb. Technol. 28 2001 129 138
14. L. Hilden, G. Johansson, G. Pettersson, J. Li, P. Ljungquist, and G. Henriksson Do the extracellular enzymes cellobiose dehydrogenase and manganese peroxidase form a pathway in lignin biodegradation? FEBS Lett. 477 2000 79 83
15. S.D. Mansfield, E. De Jong, and J.N. Saddler Cellobiose dehydrogenase, an active agent in cellulose depolymerization Appl. Environ. Microbiol. 63 1997 3804 3809
16. M. Elmgren, M. Nordling, and S.E. Lindquist The influence of flow rate on biosensors based on redox enzymes incorporated in a redox polymer mounted in a thin-layer flow cell Anal. Biochem. 215 1993 261 265
17. A. Lindgren, L. Stoica, T. Ruzgas, A. Ciucu, and L. Gorton Development of a cellobiose dehydrogenase modified electrode for amperometric detection of diphenols Analyst 124 1999 527 532
18. M.D. Cameron, Z.D. Post, J.D. Stahl, J. Haselbach, and S.D. Aust Cellobiose dehydrogenase-dependent biodegradation of polyacrylate polymers by Phanerochaete chrysosporium Environ. Sci. Pollut. Res. Int. 7 2000 130 134
19. I. Ciullini, S. Tilli, A. Scozzafava, and F. Briganti Fungal laccase, cellobiose dehydrogenase, and chemical mediators: combined actions for the decolorization of different classes of textile dyes Bioresour. Technol. 99 2008 7003 7010
20. W. Bao, S.N. Usha, and V. Renganathan Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium Arch. Biochem. Biophys. 300 1993 705 713
21. R. Ludwig, A. Salamon, J. Varga, M. Zamocky, C.K. Peterbauer, K.D. Kulbe, and D. Haltrich Characterisation of cellobiose dehydrogenases from the white-rot fungi Trametes pubescens and Trametes villosa Appl. Microbiol. Biotechnol. 64 2004 213 222
22. M. Raices, E. Paifer, J. Cremata, R. Montesino, J. Stahlberg, C. Divne, I.J. Szabo, G. Henriksson, G. Johansson, and G. Pettersson Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium FEBS Lett. 369 1995 233 238
23. B. Li, S.R. Nagalla, and V. Renganathan Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants Appl. Environ. Microbiol. 63 1997 796 799 (Pubitemid 27121378)
24. T.J. Dumonceaux, K.A. Bartholomew, T.C. Charles, S.M. Moukha, and F.S. Archibald Cloning and sequencing of a gene encoding cellobiose dehydrogenase from Trametes versicolor Gene 210 1998 211 219
25. M. Yoshida, T. Ohira, K. Igarashi, H. Nagasawa, and M. Samejima Molecular cloning and characterization of a cDNA encoding cellobiose dehydrogenase from the wood-rotting fungus Grifola frondosa FEMS Microbiol. Lett. 217 2002 225 230
26. T. Kajisa, M. Yoshida, K. Igarashi, A. Katayama, T. Nishino, and M. Samejima Characterization and molecular cloning of cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana J. Biosci. Bioeng. 98 2004 57 63
27. P.C. Stapleton, M.M. O'Brien, J. O'Callaghan, and A.D.W. Dobson Molecular cloning of the cellobiose dehydrogenase gene from Trametes versicolor and expression in Pichia pastoris Enzyme Microb. Technol. 34 2004 55 63
28. M. Zamocky, C. Schumann, C. Sygmund, J. O'Callaghan, A.D. Dobson, R. Ludwig, D. Haltrich, and C.K. Peterbauer Cloning, sequence analysis and heterologous expression in Pichia pastoris of a gene encoding a thermostable cellobiose dehydrogenase from Myriococcum thermophilum Protein Expr. Purif. 59 2008 258 265
29. G. Canevascini, P. Borer, and J.L. Dreyer Cellobiose dehydrogenases of Sporotrichum-(Chrysosporium) - thermophile Eur. J. Biochem. 198 1991 43 52
30. C. Tian, W.T. Beeson, A.T. Iavarone, J. Sun, M.A. Marletta, J.H. Cate, and N.L. Glass Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa Proc. Natl. Acad. Sci. USA 106 2009 22157 22162
31. M. Zamocky, R. Ludwig, C. Peterbauer, B.M. Hallberg, C. Divne, P. Nicholls, and D. Haltrich Cellobiose dehydrogenase - a flavocytochrome from wood-degrading, phytopathogenic and saprotrophic fungi Curr. Protein Pept. Sci. 7 2006 255 280
32. H.J. Vogel A convenient growth medium for Neurospora Microbiol. Genet. Bull. 13 1956 42 46
33. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press New York
34. M.M. Bradford Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding Anal. Biochem. 72 1976 248 254
35. J.E. Galagan, S.E. Calvo, K.A. Borkovich, E.U. Selker, N.D. Read, D. Jaffe, W. FitzHugh, L.J. Ma, S. Smirnov, S. Purcell, B. Rehman, T. Elkins, R. Engels, S. Wang, C.B. Nielsen, J. Butler, M. Endrizzi, D. Qui, P. Ianakiev, D. Bell-Pedersen, M.A. Nelson, M. Werner-Washburne, C.P. Selitrennikoff, J.A. Kinsey, E.L. Braun, A. Zelter, U. Schulte, G.O. Kothe, G. Jedd, W. Mewes, C. Staben, E. Marcotte, D. Greenberg, A. Roy, K. Foley, J. Naylor, N. Stange-Thomann, R. Barrett, S. Gnerre, M. Kamal, M. Kamvysselis, E. Mauceli, C. Bielke, S. Rudd, D. Frishman, S. Krystofova, C. Rasmussen, R.L. Metzenberg, D.D. Perkins, S. Kroken, C. Cogoni, G. Macino, D. Catcheside, W. Li, R.J. Pratt, S.A. Osmani, C.P. DeSouza, L. Glass, M.J. Orbach, J.A. Berglund, R. Voelker, O. Yarden, M. Plamann, S. Seiler, J. Dunlap, A. Radford, R. Aramayo, D.O. Natvig, L.A. Alex, G. Mannhaupt, D.J. Ebbole, M. Freitag, I. Paulsen, M.S. Sachs, E.S. Lander, C. Nusbaum, B. Birren, The genome sequence of the filamentous fungus Neurospora crassa, Nature 422 (2003) 859-868. (Pubitemid 36520034)
36. M. Yoshida, T. Ohira, K. Igarashi, H. Nagasawa, K. Aida, B.M. Hallberg, C. Divne, T. Nishino, and M. Samejima Production and characterization of recombinant Phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast Pichia pastoris Biosci. Biotechnol. Biochem. 65 2001 2050 2057
37. G. Henriksson, P. Ander, B. Pettersson, and G. Pettersson Cellobiose dehydrogenase (cellobiose oxidase) from Phanerochaete chrysosporium as a wood degrading enzyme - studies on cellulose, xylan and synthetic lignin Appl. Microbiol. Biotechnol. 42 1995 790 796
38. J. Fang, Y.B. Qu, and P.J. Gao Wide distribution of cellobiose-oxidizing enzymes in wood-rot fungus indicates a physiological importance in lignocellulosics degradation Biotechnol. Technol. 11 1997 195 197
39. U. Temp, and C. Eggert Novel interaction between laccase and cellobiose dehydrogenase during pigment synthesis in the white rot fungus Pycnoporus cinnabarinus Appl. Environ. Microbiol. 65 1999 389 395
40. S.S. Subramaniam, S.R. Nagalla, and V. Renganathan Cloning and characterization of a thermostable cellobiose dehydrogenase from Sporotrichum thermophile Arch. Biochem. Biophys. 365 1999 223 230
41. J. Fang, W. Liu, and P.J. Gao Cellobiose dehydrogenase from Schizophyllum commune: purification and study of some catalytic, inactivation, and cellulose-binding properties Arch. Biochem. Biophys. 353 1998 37 46