A low-cost hydrogel chip for SNP typing by the incorporation of cy5-dCTP into label-free allele-specific probes hybridizing to gel-immobilized targets

Journal of Nanoscience and Nanotechnology

Volumn 12, Issue 9, 2012, Pages 6887-6892

  Wang, Hui ^a,b   Chen, Zhiyao ^c   Fu, Xiaoying ^b   Li, Youzhi ^b   Tang, Xiaowen ^a,b   Wu, Haiping ^b   Zou, Bingjie ^c   Zhou, Guohua ^a,b,c  

^a NANJING MEDICAL UNIVERSITY   (China)

^b HUADONG RESEARCH INSTITUTE FOR MEDICINE AND BIOTECHNICS   (China)

^c MEDICAL SCHOOL OF NANJING UNIVERSITY   (China)

Author keywords

Electrophoresis; Extension Reaction; Polyacrylamide Gel Microarray; Single Nucleotide Polymorphism (SNP)

Indexed keywords

3-DIMENSIONAL; ACRYLAMIDE MONOMERS; AMPLICONS; CROSS CONTAMINATION; EXTENSION REACTION; HIGH SPECIFICITY; HIGH-THROUGHPUT; LABEL FREE; LOW BACKGROUND; LOW COSTS; PCR PRODUCTS; PERSONALIZED MEDICINES; POLYACRYLAMIDE GELS; PROOF OF CONCEPT; PYROSEQUENCING; SINGLE-NUCLEOTIDE POLYMORPHISMS; SINGLE-STRANDED DNA;

AMIDES; COSTS; DIAGNOSIS; DNA; ELECTROPHORESIS; FLUORESCENCE; HYDROGELS; POLYMERASE CHAIN REACTION; PROBES; PURIFICATION; THREE DIMENSIONAL;

GENES;

CARBOCYANINE; CY5 DCTP; CY5-DCTP; DEOXYCYTIDINE PHOSPHATE;

ALLELE; ARTICLE; DNA PROBE; HYDROGEL; METABOLISM; SINGLE NUCLEOTIDE POLYMORPHISM;

ALLELES; CARBOCYANINES; DEOXYCYTOSINE NUCLEOTIDES; DNA PROBES; HYDROGELS; POLYMORPHISM, SINGLE NUCLEOTIDE;

EID: 84866132514     PISSN: 15334880     EISSN: 15334899     Source Type: Journal    
DOI: 10.1166/jnn.2012.6558     Document Type: Article

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