Characterization of dynamic and steady-state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry

Electrophoresis

Volumn 25, Issue 15, 2004, Pages 2526-2532

  Schulenberg, Birte ^a   Goodman, Terrie N ^a   Aggeler, Robert ^b   Capaldi, Roderick A ^b   Patton, Wayne F ^c  

^a MOLECULAR PROBES INC   (United States)

^b UNIVERSITY OF OREGON   (United States)

^c PERKINELMER INC   (United States)

Author keywords

Phosphopeptides; Phosphoproteins; Phosphoproteome; Phosphoproteomics; Signal transduction

Indexed keywords

BACTERIOPHAGES; DESORPTION; DIAMONDS; ENZYMES; FLUORESCENCE; MASS SPECTROMETRY; MITOCHONDRIA; PEPTIDES; PHOSPHORYLATION; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

CELLULAR ACTIVITIES; PHOSPHOPEPTIDES; PHOSPHOPROTEIN; PHOSPHOPROTEOMES; PHOSPHOPROTEOMICS; PROTEIN FUNCTIONS; PROTEIN KINASE; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION PATHWAYS; STEADY STATE;

SIGNAL TRANSDUCTION;

CYCLIC AMP DEPENDENT PROTEIN KINASE; FLUORESCENT DYE; PHOSPHOPROTEIN; PHOSPHOSERINE; PHOSPHOTHREONINE; PHOSPHOTYROSINE; PRO Q DIAMOND; PROTEIN KINASE; PROTEIN SUBUNIT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CHEMICAL ANALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE ANALYSIS; HEART MITOCHONDRION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MITOCHONDRIAL RESPIRATION; MOLECULAR DYNAMICS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEOMICS; REGULATORY MECHANISM; RESPIRATORY CHAIN; SIGNAL TRANSDUCTION; STEADY STATE;

EID: 4444305698     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200406007     Document Type: Article

References (18)

1. Patton, W., J. Chromatogr. B 2002, 771, 3-31.
2. Patton, W., Beechem, J., Curr. Opin. Chem. Biol. 2002, 6, 63-69.
3. Steinberg, T., Agnew, B., Gee, K., Leung, W., Goodman, T., Schulenberg, B., Hendrickson, J., Beechem, J., Haugland, R., Patton, W., Proteomics 2003, 3, 1128-1144.
4. Martin, K., Steinberg, T., Cooley, L., Gee, K., Beechem, J., Patton, W., Proteomics 2003, 3, 1244-1255.
5. Martin, K., Steinberg, T., Goodman, T., Schulenberg, B., Kilgore, J., Gee, K., Beechem, J., Patton, W., Comb. Chem. High-Throughput Screen. 2003, 6, 79-99.
6. Schulenberg, B., Aggeler, B., Beechem, J., Capaldi, R., Patton, W., J. Biol. Chem. 2003, 278, 27251-27255.
7. Hanson, B., Schulenbery, B., Patton, W., Capaldi, R., Electrophoresis 2001, 22, 950-959.
8. Wessel, D., Flügge, U. I., Anal. Biochem. 1984, 138, 141-143.
9. Berggren, K., Chernokalskaya, E., Steinberg, T., Kemper, C., Lopez, M., Diwu, Z., Haugland, R., Patton, W., Electrophoresis 2000, 21, 2509-2521.
10. Berggren, K., Schulenberg, B., Lopez, M., Steinberg, T., Bogdanova, A., Smejkal, G., Wang, A., Patton, W., Proteomics 2002, 2, 486-498.
11. Smilansky Z., Electrophoresis 2001, 22,1616-1626.
12. Martin, K., Hart, C., Liu, J., Leung, W., Patton, W., Proteomics 2003, 3, 1215-1227.
13. Aggeler, R., Coons, J., Taylor, S. W., Ghosh, S. S., Garcia, J. J., Capaldi, R. A., Marusich, M. F., J. Biol. Chem. 2002, 277, 33906-33912.
14. Scacco, S., Vergari, R., Scarpulla, R., Technikova-Dobrova, Z., Sardanelli, A., Lambo, R., Lorusso, V., Papa, S., J. Biol. Chem. 2000, 275, 17578-17582.
15. Sardanelli, A. M., Technikova-Dobrova, Z., Scacco, S. C., Speranza, F., Papa, S., FEBS Lett. 1995, 377, 470-474.
16. Sardanelli, A. M., Technikova-Dobrova, Z., Speranza, F., Mazzocca, A., Scacco, S., Papa, S., FEBS Lett. 1996, 396, 276-278.
17. Technikova-Dobrova, Z., Sardanelli, A. M., Speranza, F., Scacco, S., Signorile, A., Lorusso, V., Papa, S., Biochemistry 2001, 40, 13941-13947.
18. Chen, R., Fearnley, I., Peak-Chew, S. Y., Walker, J. E., J. Biol. Chem. 2004, in press.