Directed evolution of a β-glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars

Protein Engineering, Design and Selection

Volumn 25, Issue 9, 2012, Pages 465-472

  Shim, Jae Hoon ^a,b   Chen, Hong Ming ^a   Rich, Jamie R ^a   Goddard Borger, Ethan D ^a   Withers, Stephen G ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b Hallym University   (South Korea)

Author keywords

3 O methyl glucose; directed evolution; glucosidase; glycosynthase; site saturation mutagenesis

Indexed keywords

ACTIVE SITE; AGROBACTERIUM SP; DIRECTED EVOLUTION; GALACTOPYRANOSIDE; GALACTOSIDASES; GLUCOSIDASE; GLYCANS; GLYCOSIDASES; GLYCOSYL; GLYCOSYNTHASE; HOMOLOGY MODELS; HYDROXYL GROUPS; SATURATION MUTAGENESIS; WILD TYPES;

AMINO ACIDS; CARBOHYDRATES; GENES; GLUCOSE; MUTAGENESIS;

ENZYMES;

3 METHYLGLUCOSE; BACTERIAL ENZYME; BETA GLYCOSIDASE; GALACTOSIDASE; GLUCOSIDASE; GLYCOSIDASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SYNTHESIS; MUTAGENESIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; RHIZOBIUM;

3-O-METHYLGLUCOSE; AGROBACTERIUM; BETA-GLUCOSIDASE; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; SUBSTRATE SPECIFICITY;

AGROBACTERIUM SP.;

EID: 84865721253     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs045     Document Type: Article

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