Using FLIM-FRET to Measure Conformational Changes of Transglutaminase Type 2 in Live Cells

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Caron, Nicholas S ^a   Munsie, Lise N ^a   Keillor, Jeffrey W ^b   Truant, Ray ^a  

^a MCMASTER UNIVERSITY   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE DIPHOSPHATE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE 2;

ARTICLE; BIOSENSOR; CALCIUM BINDING; CALCIUM CELL LEVEL; CELL STRESS; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FORSTER RESONANCE ENERGY TRANSFER; NUCLEOTIDE BINDING SITE; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS;

ANIMALS; CELL LINE; CELL SURVIVAL; ENZYME INHIBITORS; FLUORESCENCE RESONANCE ENERGY TRANSFER; GTP-BINDING PROTEINS; HUMANS; MICE; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; POINT MUTATION; PROTEIN CONFORMATION; STRESS, PHYSIOLOGICAL; TRANSGLUTAMINASES;

EID: 84865659035     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0044159     Document Type: Article

References (32)

1. Folk JE, Finlayson JS, (1977) The epsilon-(gamma-glutamyl)lysine crosslink and the catalytic role of transglutaminases. AdvProtein Chem 31: 1-133.
2. Griffin M, Casadio R, Bergamini CM, (2002) Transglutaminases: nature's biological glues. BiochemJ 368: 377-396.
3. Chen JS, Mehta K, (1999) Tissue transglutaminase: an enzyme with a split personality. IntJBiochemCell Biol 31: 817-836.
4. Nakaoka H, Perez DM, Baek KJ, Das T, Husain A, et al. (1994) Gh: a GTP-binding protein with transglutaminase activity and receptor signaling function. Science 264: 1593-1596.
5. Begg GE, Holman SR, Stokes PH, Matthews JM, Graham RM, et al. (2006) Mutation of a critical arginine in the GTP-binding site of transglutaminase 2 disinhibits intracellular cross-linking activity. JBiol Chem 281: 12603-12609.
6. Pinkas DM, Strop P, Brunger AT, Khosla C, (2007) Transglutaminase 2 undergoes a large conformational change upon activation. PLoSBiol 5: e327.
7. Gundemir S, Johnson GV, (2009) Intracellular localization and conformational state of transglutaminase 2: implications for cell death. PLoSOne 4: e6123.
8. Van Der Meer BW, Coker I, Chen SYS (1994) Resonance Energy Transfer: Theory and Data. VCH.
9. Rizzo MA, Springer G, Segawa K, Zipfel WR, Piston DW, (2006) Optimization of pairings and detection conditions for measurement of FRET between cyan and yellow fluorescent proteins. MicroscMicroanal 12: 238-254.
10. Rizzo MA, Springer GH, Granada B, Piston DW, (2004) An improved cyan fluorescent protein variant useful for FRET. NatBiotechnol 22: 445-449.
11. Wallrabe H, Periasamy A, (2010) Imaging Protein Molecules Using FRET and FLIM Microscopy. CurrOpinBiotech 16: 19-27.
12. Wallrabe H, Periasamy A, (2005) Imaging protein molecules using FRET and FLIM microscopy. CurrOpinBiotechnol 16: 19-27.
13. Chen Y, Mills JD, Periasamy A, (2003) Protein localization in living cells and tissues using FRET and FLIM. Differentiation 71: 528-541.
14. Majoul I, Straub M, Duden R, Hell SW, Soling HD, (2002) Fluorescence resonance energy transfer analysis of protein-protein interactions in single living cells by multifocal multiphoton microscopy. J Biotechnol 82: 267-277.
15. Alcor D, Calleja V, Larijani B, (2009) Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy. Methods Mol Biol 462: 307-343.
16. Duncan RR, Bergmann A, Cousin MA, Apps DK, Shipston MJ, (2004) Multi-dimensional time-correlated single photon counting (TCSPC) fluorescence lifetime imaging microscopy (FLIM) to detect FRET in cells. JMicrosc 215: 1-12.
17. DeLano WL (2002) The PyMOL Molecular Graphics System. San Carlos, CA, USA: DeLano Scientific.
18. Ruan Q, Tucholski J, Gundemir S, Johnson Voll GV, (2008) The Differential Effects of R580A Mutation on Transamidation and GTP Binding Activity of Rat and Human Type 2 Transglutaminase. IntJClinExpMed 1: 248-259.
19. Murthy SN, Iismaa S, Begg G, Freymann DM, Graham RM, et al. (2002) Conserved tryptophan in the core domain of transglutaminase is essential for catalytic activity. ProcNatlAcadSciUSA 99: 2738-2742.
20. Sorger PK, (1991) Heat shock factor and the heat shock response. Cell 65: 363-366.
21. Lunec J, Cresswell SR, (1983) Heat-induced thermotolerance expressed in the energy metabolism of mammalian cells. RadiatRes 93: 588-597.
22. Drummond IA, McClure SA, Poenie M, Tsien RY, Steinhardt RA, (1986) Large changes in intracellular pH and calcium observed during heat shock are not responsible for the induction of heat shock proteins in Drosophila melanogaster. MolCell Biol 6: 1767-1775.
23. Wiemann M, Busselberg D, Schirrmacher K, Bingmann D, (1998) A calcium release activated calcium influx in primary cultures of rat osteoblast-like cells. CalcifTissue Int 63: 154-159.
24. Colak G, Keillor JW, Johnson GV, (2011) Cytosolic guanine nucledotide binding deficient form of transglutaminase 2 (R580a) potentiates cell death in oxygen glucose deprivation. PLoSOne 6: e16665.
25. Keillor JW, Chica RA, Chabot N, Vinci V, Pardin C, et al. (2008) The bioorganic chemistry of transglutaminase- from mechanism to inhibition and engineering. CanJChem 86: 271-276.
26. Pardin C, Roy I, Lubell WD, Keillor JW, (2008) Reversible and competitive cinnamoyl triazole inhibitors of tissue transglutaminase. ChemBiolDrug Des 72: 189-196.
27. Kim SY, Jeitner TM, Steinert PM, (2001) Transglutaminases and Disease. NeurochemInt 40: 85-103.
28. Munsie L, Caron N, Atwal RS, Marsden I, Wild EJ, et al. (2011) Mutant huntingtin causes defective actin remodeling during stress: defining a new role for transglutaminase 2 in neurodegenerative disease. HumMolGenet 20: 1937-1951.
29. Cooper AJ, Jeitner TM, Blass JP, (2002) The role of transglutaminases in neurodegenerative diseases: overview. NeurochemInt 40: 1-5.
30. Jeitner TM, Pinto JT, Krasnikov BF, Horswill M, Cooper AJ, (2009) Transglutaminases and neurodegeneration. JNeurochem 109 (Suppl 1):: 160-166.
31. Kenworthy AK, (2001) Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. Methods 24: 289-296.
32. Trettel F, Rigamonti D, Hilditch-Maguire P, Wheeler VC, Sharp AH, et al. (2000) Dominant phenotypes produced by the HD mutation in STHdh(Q111) striatal cells. HumMolGenet 9: 2799-2809.