Myofibroblasts in interstitial lung diseases show diverse electron microscopic and invasive features

Laboratory Investigation

Volumn 92, Issue 9, 2012, Pages 1270-1284

  Karvonen, Henna M ^a   Lehtonen, Siri T ^a,b   Sormunen, Raija T ^b   Harju, Terttu H ^a   Lappi Blanco, Elisa ^a,b   Bloigu, Risto S ^b   Kaarteenaho, Riitta L ^a  

^a OULU UNIVERSITY HOSPITAL   (Finland)

^b UNIVERSITY OF OULU   (Finland)

Author keywords

alpha smooth muscle actin; bronchoalveolar lavage; fibronectin; fibronexus; immunoelectron microscopy; pulmonary fibrosis

Indexed keywords

ACTIN; ALPHA SMOOTH MUSCLE ACTIN; FIBRONECTIN; MESSENGER RNA;

ARTICLE; BRONCHOSCOPY; CELL CULTURE; CELL JUNCTION; ELECTRON MICROSCOPY; FIBROBLAST; FIBROSING ALVEOLITIS; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOELECTRON MICROSCOPY; INTERSTITIAL LUNG DISEASE; LUNG LAVAGE; MAJOR CLINICAL STUDY; MYOFIBROBLAST; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; WESTERN BLOTTING;

ACTINS; BASE SEQUENCE; BIOPSY; BLOTTING, WESTERN; BRONCHOALVEOLAR LAVAGE FLUID; DNA PRIMERS; HUMANS; LUNG DISEASES, INTERSTITIAL; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, IMMUNOELECTRON; MYOFIBROBLASTS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84865620039     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2012.95     Document Type: Article

References (31)

1. Majno G, Shea SM, Leventhal M. Endothelial contraction induced by histamine-type mediators: an electron microscopic study. J Cell Biol 1969;42:647-672.
2. Majno G, Gabbiani G, Hirschel BJ, et al. Contraction of granulation tissue in vitro: similarity to smooth muscle. Science 1971;173:548-550.
3. Gabbiani G, Ryan GB, Majne G. Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction. Experientia 1971;27:549-550.
4. Singer II. The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts. Cell 1979;16:675-685. (Pubitemid 9140470)
5. Singer II, Kawka DW, Kazazis DM, et al. In vivo co-distribution of fibronectin and actin fibers in granulation tissue: immunofluorescence and electron microscope studies of the fibronexus at the myofibroblast surface. J Cell Biol 1984;98:2091-2106. (Pubitemid 14121840)
6. Singer II, Kazazis DM, Kawka DW. Localization of the fibronexus at the surface of granulation tissue myofibroblasts using double-label immunogold electron microscopy on ultrathin frozen sections. Eur J Cell Biol 1985;38:94-101. (Pubitemid 15045724)
7. Desmouliere A, Chaponnier C, Gabbiani G. Tissue repair, contraction, and the myofibroblast. Wound Repair Regen 2005;13:7-12. (Pubitemid 40250424)
8. Eyden B. The myofibroblast: phenotypic characterization as a prerequisite to understanding its functions in translational medicine. J Cell Mol Med 2008;12:22-37. (Pubitemid 351430454)
9. Eyden B. The myofibroblast: an assessment of controversial issues and a definition useful in diagnosis and research. Ultrastruct Pathol 2001;25:39-50. (Pubitemid 32182093)
10. Eyden B. The fibronexus in reactive and tumoral myofibroblasts: further characterisation by electron microscopy. Histol Histopathol 2001;16:57-70. (Pubitemid 32094023)
11. Eyden B, Banerjee SS, Shenjere P, et al. The myofibroblast and its tumours. J Clin Pathol 2009;62:236-249.
12. Eyden BP. Brief review of the fibronexus and its significance for myofibroblastic differentiation and tumor diagnosis. Ultrastruct Pathol 1993;17:611-622. (Pubitemid 24028743)
13. Kaarteenaho-Wiik R, Sademies O, Paakko P, et al. Extracellular matrix proteins and myofibroblasts in granulomas of sarcoidosis, atypical mycobacteriosis, and tuberculosis of the lung. Hum Pathol 2007;38:147-153. (Pubitemid 44880210)
14. Kaarteenaho-Wiik R, Paakko P, Sormunen R. Ultrastructural features of lung fibroblast differentiation into myofibroblasts. Ultrastruct Pathol 2009;33:6-15.
15. Technical recommendations and guidelines for bronchoalveolar lavage (BAL). Report of the European Society of Pneumology Task Group. Eur Respir J 1989;2:561-585.
16. Silcocks PB. Measuring repeatability and validity of histological diagnosis\a brief review with some practical examples. J Clin Pathol 1983;36:1269-1275. (Pubitemid 14205410)
17. Slot JW, Geuze HJ. A new method of preparing gold probes for multiple-labeling cytochemistry. Eur J Cell Biol 1985;38:87-93. (Pubitemid 15045723)
18. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 2001;25:402-408. (Pubitemid 34164012)
19. Rothman KJ. No adjustments are needed for multiple comparisons. Epidemiology 1990;1:43-46. (Pubitemid 20255621)
20. Badri L, FAU-Murray S, Murray S, et al. Mesenchymal stromal cells in bronchoalveolar lavage as predictors of bronchiolitis obliterans syndrome. Am J Respir Crit Care Med 2011;183:1062-1070.
21. Rock JR, Barkauskas CE, Cronce MJ, et al. Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition. Proc Natl Acad Sci USA 2011;108: E1475-E1483.
22. Hinz B, Gabbiani G. Cell-matrix and cell-cell contacts of myofibroblasts: role in connective tissue remodeling. Thromb Haemost 2003;90: 993-1002. (Pubitemid 38004416)
23. Hinz B, Pittet P, Smith-Clerc J, et al. Myofibroblast development is characterized by specific cell-cell adherens junctions. Mol Biol Cell 2004;15:4310-4320. (Pubitemid 39122032)
24. Gabbiani G, Chaponnier C, Huttner I. Cytoplasmic filaments and gap junctions in epithelial cells and myofibroblasts during wound healing. J Cell Biol 1978;76:561-568. (Pubitemid 8295898)
25. Follonier L, Schaub S, Meister JJ, et al. Myofibroblast communication is controlled by intercellular mechanical coupling. J Cell Sci 2008;121(Pt 20):3305-3316.
26. White ES, Thannickal VJ, Carskadon SL, et al. Integrin alpha4beta1 regulates migration across basement membranes by lung fibroblasts: a role for phosphatase and tensin homologue deleted on chromosome 10. Am J Respir Crit Care Med 2003;168:436-442. (Pubitemid 37046872)
27. Lovgren AK, Kovacs JJ, Xie T, et al. Beta-arrestin deficiency protects against pulmonary fibrosis in mice and prevents fibroblast invasion of extracellular matrix. Sci Transl Med 2011;3:74ra23.
28. Fireman E, Kivity S, Shahar I, et al. Secretion of stem cell factor by alveolar fibroblasts in interstitial lung diseases. Immunol Lett 1999;67:229-236. (Pubitemid 29219769)
29. Fireman E, Shahar I, Shoval S, et al. Morphological and biochemical properties of alveolar fibroblasts in interstitial lung diseases. Lung 2001;179:105-117. (Pubitemid 33022276)
30. Larsen K, Tufvesson E, Malmstrom J, et al. Presence of activated mobile fibroblasts in bronchoalveolar lavage from patients with mild asthma. Am J Respir Crit Care Med 2004;170:1049-1056. (Pubitemid 39488616)
31. Larsen K, Malmstrom J, Wildt M, et al. Functional and phenotypical comparison of myofibroblasts derived from biopsies and bronchoalveolar lavage in mild asthma and scleroderma. Respir Res 2006;7:11.