Presence of activated mobile fibroblasts in bronchoalveolar lavage from patients with mild asthma

American Journal of Respiratory and Critical Care Medicine

Volumn 170, Issue 10, 2004, Pages 1049-1056

  Larsen, Kristoffer ^a   Tufvesson, Ellen ^b   Malmström, Johan ^b   Mörgelin, Matthias ^b   Wildt, Marie ^b   Andersson, Annika ^b   Lindström, Anna ^b   Malmström, Anders ^b   Löfdahl, Claes Göran ^b   Marko Varga, György ^b   Bjermer, Leif ^b   Westergren Thorsson, Gunilla ^b  

^a LUND UNIVERSITY   (Sweden)

^b NONE

Author keywords

Asthma; Bronchoalveolar lavage fluid; Fibroblast; Fibrosis; Migration

Indexed keywords

BIGLYCAN; CHLORIDE ION; COFILIN; DECORIN; HEAT SHOCK PROTEIN 20; PERLECAN; RAC1 PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; VERSICAN;

ADULT; AGED; ARTICLE; ASTHMA; BRONCHUS BIOPSY; CELL ACTIVITY; CELL MIGRATION; CELL MOTILITY; CLINICAL ARTICLE; CYTOPLASM; EOSINOPHIL; FEMALE; FIBROBLAST; HUMAN; LUNG FIBROSIS; LUNG LAVAGE; MALE; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RESPIRATORY EPITHELIUM; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ADOLESCENT; ADULT; AGED; ASTHMA; BIOPSY, NEEDLE; BLOTTING, WESTERN; BRONCHIAL HYPERREACTIVITY; BRONCHIAL PROVOCATION TESTS; BRONCHOALVEOLAR LAVAGE FLUID; CELL MOVEMENT; CELLS, CULTURED; COHORT STUDIES; FEMALE; FIBROBLASTS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MICROSCOPY, ELECTRON; MIDDLE AGED; PROBABILITY; PROGNOSIS; SENSITIVITY AND SPECIFICITY; SEVERITY OF ILLNESS INDEX;

EID: 8444250651     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/rccm.200404-507OC     Document Type: Article

References (35)

1. Cohn L, Elias JA, Chupp GL. Asthma: mechanisms of disease persistence and progression. Anna Rev Immunol 2004;22:789-815.
2. Jeffery PK. Remodeling in asthma and chronic obstructive lung disease. Am J Respir Crit Care Med 2001;164:S28-S38.
3. Tamby MC, Chanseaud Y, Guillevin L, Mouthon L. New insights into the pathogenesis of systemic sclerosis. Autoimmun Rev 2003;2:152-157.
4. Ricciardolo FL, Di Stefano A, van Krieken JH, Sont JK, van Schadewijk A, Rabe KF, Donner CF, Hiemstra PS, Sterk PJ, Mauad T. Proliferation and inflammation in bronchial epithelium after allergen in atoic asthmatics. Clin Exp Allergy 2003;33:905-911.
5. Holgate ST. Asthma: more than an inflammatory disease. Curr Opin Allergy Clin Immunol 2002;2:27-29.
6. Huang J, Olivenstein R, Taha R, Hamid Q, Ludwig M. Enhanced proteoglycan deposition in the airway wall of atopic asthmatics. Am J Respir Crit Care Med 1999;160:725-729.
7. Puddicombe SM, Polosa R, Richter A, Krishna MT, Howarth PH, Hoigate ST, Davies DE. Involvement of the epidermal growth factor receptor in epithelial repair in asthma. FASEB J 2000;14:1362-1374.
8. Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol 2002;3:349-363.
9. Fukata M, Nakagawa M, Kaibuchi K. Roles of Rho-family GTPases in cell polarisation and directional migration. Curr Opin Cell Biol 2003; 15:590-597.
10. Serini G, Gabbiani G. Mechanisms of myofibroblast activity and phenotypic modulation. Exp Cell Res 1999;250:273-283.
11. Westergren-Thorsson G, Chakir J, Lafreniere-Allard MJ, Boulet LP, Tremblay GM. Correlation between airway responsiveness and proteoglycan production by bronchial fibroblasts from normal and asthmatic subjects. Int J Biochem Cell Biol 2002;34:1256-1267.
12. Hocking DC. Fibronectin matrix deposition and cell contractility: implications for airway remodeling in asthma. Chest 2002;122:275S-278S.
13. Malmstrom J, Tufvesson E, Lofdahl CG, Hansson L, Marko-Varga G, Westergren-Thorsson G. Activation of platelet-derived growth factor pathway in human asthmatic pulmonary-derived mesenchymal cells. Electrophoresis 2003;24:276-285.
14. Silverman ES, Palmer LJ, Subramaniam V, Hallock A, Mathew S, Vallone J, Faffe DS, Shikanai T, Raby BA, Weiss ST, et al. Transforming growth factor-beta1 promoter polymorphism C-509T is associated with asthma. Am J Respir Crit Care Med 2004;169:214-219.
15. Flood-Page P, Menzies-Gow A, Phipps S, Ying S, Wangoo A, Ludwig MS, Barnes N, Robinson D, Kay AB. Anti-IL-5 treatment reduces deposition of ECM proteins in the bronchial subepithelial basement membrane of mild atopic asthmatics. J Clin Invest 2003;112:1029-1036.
16. Malmstrom J, Larsen K, Hansson L, Lofdahl CG, Norregard-Jensen O, Marko-Varga G, Westergren-Thorsson G. Proteoglycan and proteome profiling of central human pulmonary fibrotic tissue utilizing miniaturized sample preparation: a feasibility study. Proteomics 2002;2:394-404.
17. Redington AE, Roche WR, Madden J, Frew AJ, Djukanovic R, Holgate ST, Howarth PH. Basic fibroblast growth factor in asthma: measurement in bronchoalveolar lavage fluid basally and following allergen challenge. J Allergy Clin Immunol 2001;107:384-387.
18. Tufvesson E, Westergren-Thorsson G. Biglycan and decorin induce morphological and cytoskeletal changes involving signalling by the small GTPases RhoA and Rac1 resulting in lung fibroblast migration. J Cell Sci 2003;116:4857-4864.
19. Tufvesson E, Malmstrom J, Marko-Varga G, Westergren-Thorsson G. Biglycan isoforms with differences in polysaccharide substitution and core protein in human lung fibroblasts. Eur J Biochem 2002;269:3688-3696.
20. Brewster CE, Howarth PH, Djukanovic R, Wilson J, Holgate ST, Roche WR. Myofibroblasts and subepithelial fibrosis in bronchial asthma. Am J Respir Cell Mol Biol 1990;3:507-511.
21. Ludwicka A, Ohba T, Trojanowska M, Yamakage A, Strange C, Smith EA, LeRoy EC, Sutherland S, Silver RM. Elevated levels of platelet derived growth factor and transforming growth factor-beta 1 in bronchoalveolar lavage fluid from patients with scleroderma. J Rheumatol 1995;22:1876-1883.
22. Ohba T, McDonald JK, Silver RM, Strange C, LeRoy EC, Ludwicka A. Scleroderma bronchoalveolar lavage fluid contains thrombin, a mediator of human lung fibroblast proliferation via induction of platelet-derived growth factor alpha-receptor. Am J Respir Cell Mol Biol 1994;10:405-412.
23. Lazaar AL, Panettieri RA Jr. Is airway remodeling clinically relevant in asthma? Am J Med 2003;115:652-659.
24. Kalluri R, Neilson EG. Epithelial-mesenchymal transition and its implications for fibrosis. J Clin Invest 2003;112:1776-1784.
25. Schmidt M, Sun G, Stacey MA, Mori L, Mattoli S. Identification of circulating fibrocytes as precursors of bronchial myofibroblasts in asthma. J Immunol 2003;171:380-389.
26. Ronnov-Jessen L, Villadsen R, Edwards JC, Petersen OW. Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts. Am J Pathol 2002;161:471-480.
27. Flynn CR, Komalavilas P, Tessier D, Thresher J, Niederkofler EE, Dreiza CM, Nelson RW, Panitch A, Joshi L, Brophy CM. Transduction of biologically active motifs of the small heat shock-related protein HSP20 leads to relaxation of vascular smooth muscle. FASEB J 2003; 17:1358-1360.
28. Tessier DJ, Komalavilas P, Panitch A, Joshi L, Brophy CM. The small heat shock protein (HSP) 20 is dynamically associated with the actin cross-linking protein actinin. J Surg Res 2003;111:152-157.
29. Schoenwaelder SM, Burridge K. Bidirectional signaling between the cytoskeleton and integrins. Curr Opin Cell Biol 1999;11:274-286.
30. Wittmann T, Waterman-Storer CM. Cell motility: can Rho GTPases and microtubules point the way? J Cell Sci 2001;114:3795-3803.
31. Hamburg JR. Proteins of the ADF/cofilin family, essential regulators of actin dynamics. Annu Rev Cell Dev Biol 1999;15:185-230.
32. Venkatesan N, Roughley PJ, Ludwig MS. Proteoglycan expression in bleomycin lung fibroblasts: role of transforming growth factor-beta(1) and interferon-gamma. Am J Physiol Lung Cell Mol Physiol 2002;283: L806-L814.
33. Westergren-Thorsson G, Sime P, Jordana M, Gauldie J, Sarnstrand B, Malmstrom A. Lung fibroblast clones from normal and fibrotic subjects differ in hyaluronan and decorin production and rate of proliferation. Int J Biochem Cell Biol 2004;36:1573-1584.
34. Howarth PH. Small airways and asthma: an important therapeutic target? Am J Respir Crit Care Med 1998;157:S173.
35. Martin RJ. Therapeutic significance of distal airway inflammation in asthma. J Allergy Clin Immunol 2002;109:S447-S460.