Development and Application of Purified Tissue Dissociation Enzyme Mixtures for Human Hepatocyte Isolation

Cell Transplantation

Volumn 21, Issue 6, 2012, Pages 1245-1260

  Gramignoli, Roberto ^a   Green, Michael L ^b   Tahan, Veysel ^a   Dorko, Kenneth ^a   Skvorak, Kristen J ^a   Marongiu, Fabio ^c   Zao, Wenchen ^d   Venkataramanan, Raman ^d   Ellis, Ewa C S ^e   Geller, David ^f   Breite, Andrew G ^b   Dwulet, Francis E ^b   McCarthy, Robert C ^b   Strom, Stephen C ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b VitaCyte LLC   (United States)

^c UNIVERSITY OF CAGLIARI   (Italy)

^d UNIVERSITY OF PITTSBURGH   (United States)

^e KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^f UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

Author keywords

Collagenase; Hepatocyte isolation; Human hepatocytes

Indexed keywords

AMMONIA; COLLAGENASE; COLLAGENASE 11; COLLAGENASE MA; CYTOCHROME P450; CYTOCHROME P450 1A; CYTOCHROME P450 3A; ETHOXYRESORUFIN DEETHYLASE; NEUTRAL PROTEINASE; RESORUFIN; TESTOSTERONE; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CELL FUNCTION; CELL ISOLATION; CELL METABOLISM; CELL STRUCTURE; CELL VIABILITY; CONJUGATION; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME PURIFICATION; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; HUMAN TISSUE; LIVER CELL; LIVER RESECTION; MALE; PRIORITY JOURNAL;

CELL SEPARATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLLAGENASES; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 CYP3A; HEPATOCYTES; HUMANS; TESTOSTERONE;

EID: 84865529202     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/096368911X600939     Document Type: Article

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