Soluble expression and purification of tumor suppressor WT1 and its zinc finger domain

Protein Expression and Purification

Volumn 85, Issue 2, 2012, Pages 165-172

  Fagerlund, Robert D ^a   Ooi, Poh Ling ^a,b   Wilbanks, Sigurd M ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

^b AGENCY FOR SCIENCE   (Singapore)

Author keywords

Affinity tags; Protein DNA complex; WT1; Zinc finger

Indexed keywords

RECOMBINANT PROTEIN; WT1 PROTEIN; ZINC FINGER PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; SOLUBILITY; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; ESCHERICHIA COLI; MICE; RECOMBINANT PROTEINS; SOLUBILITY; WT1 PROTEINS; ZINC FINGERS;

EID: 84865488254     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.08.002     Document Type: Article

References (39)

1. Junien, C., Henry, I., Genetics of Wilms’ tumor: a blend of aberrant development and genomic imprinting. Kidney Int. 46 (1994), 1264–1279.
2. Ehrlich, P.F., Wilms tumor: progress and considerations for the surgeon. Surg. Oncol. 16 (2007), 157–171.
3. Kaste, S.C., Dome, J.S., Babyn, P.S., Graf, N.M., Grundy, P., Godzinski, J., Levitt, G.A., Jenkinson, H., Wilms tumour: prognostic factors, staging, therapy and late effects. Pediatr. Radiol. 38 (2008), 2–17.
4. Miwa, H., Tomlinson, G.E., Timmons, C.F., Huff, V., Cohn, S.L., Strong, L.C., Saunders, G.F., RNA expression of the WT1 gene in Wilms’ tumors in relation to histology. J. Natl Cancer Inst. 84 (1992), 181–187.
5. Pritchard-Jones, K., Fleming, S., Davidson, D., The candidate Wilms’ tumour gene is involved in genitourinary development. Nature 346 (1990), 194–197.
6. Satoh, Y., Nakadate, H., Nakagawachi, T., Higashimoto, K., Joh, K., Masaki, Z., Uozumi, J., Kaneko, Y., Mukai, T., Soejima, H., Genetic and epigenetic alterations on the short arm of chromosome 11 are involved in a majority of sporadic Wilms’ tumours. Br. J. Cancer 95 (2006), 541–547.
7. Varanasi, R., Bardeesy, N., Ghahremani, M., Petruzzi, M.J., Nowak, N., Adam, M.A., Grundy, P., Shows, T.B., Pelletier, J., Fine structure analysis of the WT1 gene in sporadic Wilms tumors. Proc. Natl. Acad. Sci. USA 91 (1994), 3554–3558.
8. Reddy, J.C., Licht, J.D., The WT1 Wilms’ tumor suppressor gene: how much do we really know?. Biochim. Biophys. Acta 1287 (1996), 1–28.
9. Menke, A., McInnes, L., Hastie, N.D., Schedl, A., The Wilms’ tumor suppressor WT1: approaches to gene function. Kidney Int. 53 (1998), 1512–1518.
10. Wang, Z.Y., Madden, S.L., Deuel, T.F., Rauscher, F.J., The Wilms’ tumor gene product, WT1, represses transcription of the platelet-derived growth factor A-chain gene. J. Biol. Chem. 267 (1992), 21999–22002.
11. Wang, Z.Y., Qiu, Q.Q., Deuel, T.F., The Wilms’ tumor gene product WT1 activates or suppresses transcription through separate functional domains. J. Biol. Chem. 268 (1993), 9172–9175.
12. Reddy, J.C., Morris, J.C., Wang, J., English, M.A., Haber, D.A., Shi, Y., Licht, J.D., WT1-mediated transcriptional activation is inhibited by dominant negative mutant proteins. J. Biol. Chem. 270 (1995), 10878–10884.
13. Moffett, P., Bruening, W., Nakagama, H., Bardeesy, N., Housman, D., Housman, D.E., Pelletier, J., Antagonism of WT1 activity by protein self-association. Proc. Natl. Acad. Sci. USA 92 (1995), 11105–11109.
14. Maheswaran, S., Englert, C., Zheng, G., Lee, S.B., Wong, J., Harkin, D.P., Bean, J., Ezzell, R., Garvin, A.J., McCluskey, R.T., DeCaprio, J.A., Haber, D.A., Inhibition of cellular proliferation by the Wilms tumor suppressor WT1 requires association with the inducible chaperone Hsp70. Genes Dev. 12 (1998), 1108–1120.
15. Wang, Z.Y., Qiu, Q.Q., Enger, K.T., Deuel, T.F., A second transcriptionally active DNA-binding site for the Wilms tumor gene product, WT1. Proc. Natl. Acad. Sci. USA 90 (1993), 8896–8900.
16. Loeb, D.M., Korz, D., Katsnelson, M., Burwell, E.A., Friedman, A.D., Sukumar, S., Cyclin E is a target of WT1 transcriptional repression. J. Biol. Chem. 277 (2002), 19627–19632.
17. Bruening, W., Moffett, P., Chia, S., Heinrich, G., Pelletier, J., Identification of nuclear localization signals within the zinc fingers of the WT1 tumor suppressor gene product. FEBS Lett. 393 (1996), 41–47.
18. Hamilton, T.B., Borel, F., Romaniuk, P.J., Comparison of the DNA binding characteristics of the related zinc finger proteins WT1 and EGR1. Biochem. 37 (1998), 2051–2058.
19. Caricasole, A., Duante, A., Larsson, S.H., Hastie, N.D., Little, M., Holmes, G., Todorov, I., Ward, A., RNA binding by the Wilms tumor suppressor zinc finger proteins. Proc. Natl. Acad. Sci. USA 93 (1996), 7562–7566.
20. Bardeesy, N., Pelletier, J., Overlapping RNA and DNA binding domains of the wt1 tumour suppressor gene product. Nucleic Acids Res. 26 (1998), 1784–1792.
21. Morrison, A., Venables, J., Dellaire, G., Ladomery, M., The Wilms’ tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain. Biochem. Cell Biol. 84 (2006), 789–798.
22. Haber, D.A., Sohn, R.L., Buckler, A.J., Pelletier, J., Call, K.M., Housman, D.E., Alternative splicing and genomic structure of the Wilms Tumor gene WT1. Proc. Natl. Acad. Sci. USA 88 (1991), 9618–9622.
23. Roberts, S.G.E., Transcriptional regulation by WT1 in development. Curr. Opin. Genetics Dev. 15 (2005), 542–547.
24. Niksic, M., Slight, J., Sanford, J.R., Caceres, J.F., Hastie, N.D., The Wilms’ tumour protein (WT1) shuttles between nucleus and cytoplasm and is present in functional polysomes. Hum. Mol. Genet. 13 (2004), 463–471.
25. Bor, Y., The Wilms’ tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron. Genes Dev. 20 (2006), 1597–1608.
26. Stoll, R., Lee, B., Debler, E., Laity, J., Wilson, I., Dyson, H., Wright, P., Structure of the Wilms’ tumor suppressor protein zinc finger domain bound to DNA. J. Mol. Biol. 372 (2007), 1227–1245.
27. Pavletich, N.P., Pabo, C.O., Zinc finger-DNA recognition: crystal structure of a Zif268–DNA complex. Science 252 (1991), 809–817.
28. Elrod-Erickson, M., Rould, M.A., Nekludova, L., Pabo, C.O., Zif268 protein–DNA complex refined at 1.6 Å: a model system for understanding zinc finger–DNA interactions. Structure 4 (1996), 1171–1180.
29. Oka, S., Shiraishi, Y., Yoshida, T., Ohkubo, T., Sugiura, Y., Kobayashi, Y., NMR structure of transcription factor Sp1 DNA binding domain. Biochemistry 43 (2004), 16027–16035.
30. Rauscher, F., Morris, J., Tournay, O., Cook, D., Binding of the Wilms’ tumor locus zinc finger protein to the EGR-1 consensus sequence. Science 250 (1990), 1259–1262.
31. Laity, J.H., Chung, J., Dyson, H.J., Wright, P.E., Alternative splicing of Wilms’ tumor suppressor protein modulates DNA binding activity through isoform-specific DNA-induced conformational changes. Biochemistry 29 (2000), 5341–5348.
32. Laity, J.H., Dyson, H.J., Wright, P.E., Molecular basis for modulation of biological function by alternative splicing of the Wilms’ tumor suppressor protein. Proc. Natl. Acad. Sci. USA 97 (2000), 11932–11935.
33. Nurmemmedov, E., Thunnissen, M., Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr. Purif. 46 (2006), 379–389.
34. Nakagama, H., Heinrich, G., Pelletier, J., Housman, D.E., Sequence and structural requirements for high-affinity DNA binding by the WT1 gene product. Mol. Cell. Biol. 15 (1995), 1489–1498.
35. Geng, J., Carstens, R.P., Two methods for improved purification of full-length mammalian proteins that have poor expression and/or solubility using standard Escherichia coli procedures. Protein Expr. Purif. 48 (2006), 142–150.
36. Dyson, M.R., Shadbolt, S.P., Vincent, K.J., Perera, R.L., McCafferty, J., Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression. BMC Biotechnol., 4, 2004, 32.
37. Tsunoda, Y., Sakai, N., Kikuchi, K., Katoh, S., Akagi, K., Miura-Ohnuma, J., Tashiro, Y., Murata, K., Shibuya, N., Katoh, E., Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli. Protein Expr. Purif. 42 (2005), 268–277.
38. Drummond, I.A., Rupprecht, H.D., Rohwernutter, P., Lopezguisa, J.M., Madden, S.L., Rauscher, F.J., Sukhatme, V.P., DNA recognition by splicing variants of the Wilms-tumor suppresson, WT1. Mol. Cell. Biol. 14 (1994), 3800–3809.
39. Iben, S., Royer-Pokora, B., Analysis of native WT1 protein from frozen human kidney and Wilms’ tumors. Oncogene 18 (1999), 2533–2536.