Elevated expression of cav-1 in a subset of SSc fibroblasts contributes to constitutive Alk1/Smad1 activation

Journal of Cellular and Molecular Medicine

Volumn 16, Issue 9, 2012, Pages 2238-2246

  Haines, Paul ^a   Hant, Faye N ^b   Lafyatis, Robert ^a   Trojanowska, Maria ^a   Bujor, Andreea M ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Caveolin 1; CCN2; Scleroderma; Smad1

Indexed keywords

ACTIVIN RECEPTOR 2; ACVRL1 PROTEIN, HUMAN; CAV1 PROTEIN, HUMAN; CAVEOLIN 1; CONNECTIVE TISSUE GROWTH FACTOR; CTGF PROTEIN, HUMAN; SMAD1 PROTEIN; SMAD1 PROTEIN, HUMAN; SMALL INTERFERING RNA; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; CELL CULTURE; CYTOLOGY; DOWN REGULATION; FIBROBLAST; GENETIC TRANSFECTION; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; METABOLISM; PATHOLOGY; PHOSPHORYLATION; SIGNAL TRANSDUCTION; SKIN; SYSTEMIC SCLEROSIS; UPREGULATION; WESTERN BLOTTING;

ACTIVIN RECEPTORS, TYPE II; BLOTTING, WESTERN; CAVEOLIN 1; CELLS, CULTURED; CONNECTIVE TISSUE GROWTH FACTOR; DOWN-REGULATION; FIBROBLASTS; HUMANS; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; PHOSPHORYLATION; RNA, SMALL INTERFERING; SCLERODERMA, SYSTEMIC; SIGNAL TRANSDUCTION; SKIN; SMAD1 PROTEIN; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA; UP-REGULATION;

EID: 84865443068     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2012.01537.x     Document Type: Article

References (27)

1. Morris E, Chrobak I, Bujor A, et al. Endoglin promotes TGF-beta/Smad1 signaling in scleroderma fibroblasts. J Cell Physiol. 2011; 226: 3340-8.
2. Bujor AM, Asano Y, Haines P, et al. The c-Abl tyrosine kinase controls protein kinase Cdelta-induced Fli-1 phosphorylation in human dermal fibroblasts. Arthritis Rheum. 2011; 63: 1729-37.
3. Pannu J, Asano Y, Nakerakanti S, et al. Smad1 pathway is activated in systemic sclerosis fibroblasts and is targeted by imatinib mesylate. Arthritis Rheum. 2008; 58: 2528-37.
4. Chen Y, Leask A, Abraham DJ, et al. Heparan sulfate-dependent ERK activation contributes to the overexpression of fibrotic proteins and enhanced contraction by scleroderma fibroblasts. Arthritis Rheum. 2008; 58: 577-85.
5. Jun JB, Kuechle M, Min J, et al. Scleroderma fibroblasts demonstrate enhanced activation of Akt (protein kinase B) in situ. J Invest Dermatol. 2005; 124: 298-303.
6. Goumans MJ, Valdimarsdottir G, Itoh S, et al. Activin receptor-like kinase (ALK)1 is an antagonistic mediator of lateral TGFbeta/ALK5 signaling. Mol Cell. 2003; 12: 817-28.
7. Lebrin F, Goumans MJ, Jonker L, et al. Endoglin promotes endothelial cell proliferation and TGF-beta/ALK1 signal transduction. EMBO J. 2004; 23: 4018-28.
8. Pannu J, Nakerakanti S, Smith E, et al. Transforming growth factor-beta receptor type I-dependent fibrogenic gene program is mediated via activation of Smad1 and ERK1/2 pathways. J Biol Chem. 2007; 282: 10405-13.
9. Abraham D. Connective tissue growth factor: growth factor, matricellular organizer, fibrotic biomarker or molecular target for anti-fibrotic therapy in SSc? Rheumatology (Oxford). 2008; 47(Suppl. 5): v8-9.
10. Patel HH, Murray F, Insel PA. Caveolae as organizers of pharmacologically relevant signal transduction molecules. Annu Rev Pharmacol Toxicol. 2008; 48: 359-91.
11. Yamamoto M, Toya Y, Schwencke C, et al. Caveolin is an activator of insulin receptor signaling. J Biol Chem. 1998; 273: 26962-8.
12. Kim S, Lee Y, Seo JE, et al. Caveolin-1 increases basal and TGF-beta1-induced expression of type I procollagen through PI-3 kinase/Akt/mTOR pathway in human dermal fibroblasts. Cell Signal. 2008; 20: 1313-9.
13. Di Guglielmo GM, Le Roy C, Goodfellow AF, et al. Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover. Nat Cell Biol. 2003; 5: 410-21.
14. Tourkina E, Gooz P, Pannu J, et al. Opposing effects of protein kinase Calpha and protein kinase Cepsilon on collagen expression by human lung fibroblasts are mediated via MEK/ERK and caveolin-1 signaling. J Biol Chem. 2005; 280: 13879-87.
15. Wang XM, Zhang Y, Kim HP, et al. Caveolin-1: a critical regulator of lung fibrosis in idiopathic pulmonary fibrosis. J Exp Med. 2006; 203: 2895-906.
16. Tourkina E, Richard M, Gooz P, et al. Antifibrotic properties of caveolin-1 scaffolding domain in vitro and in vivo. Am J Physiol Lung Cell Mol Physiol. 2008; 294: L843-61.
17. Del Galdo F, Sotgia F, de Almeida CJ, et al. Decreased expression of caveolin 1 in patients with systemic sclerosis: crucial role in the pathogenesis of tissue fibrosis. Arthritis Rheum. 2008; 58: 2854-65.
18. Santibanez JF, Blanco FJ, Garrido-Martin EM, et al. Caveolin-1 interacts and cooperates with the transforming growth factor-beta type I receptor ALK1 in endothelial caveolae. Cardiovasc Res. 2008; 77: 791-9.
19. Bujor AM, Pannu J, Bu S, et al. Akt blockade downregulates collagen and upregulates MMP1 in human dermal fibroblasts. J Invest Dermatol. 2008; 128: 1906-14.
20. Horton MR, Radler J, Gast AP. Phase behavior and the partitioning of caveolin-1 scaffolding domain peptides in model lipid bilayers. J Colloid Interface Sci. 2006; 304: 67-76.
21. Wertz JW, Bauer PM. Caveolin-1 regulates BMPRII localization and signaling in vascular smooth muscle cells. Biochem Biophys Res Commun. 2008; 375: 557-61.
22. Shi-wen X, Pennington D, Holmes A, et al. Autocrine overexpression of CTGF maintains fibrosis: RDA analysis of fibrosis genes in systemic sclerosis. Exp Cell Res. 2000; 259: 213-24.
23. Meyer C, Godoy P, Bachmann A, et al. Distinct role of endocytosis for Smad and non-Smad TGF-beta signaling regulation in hepatocytes. J Hepatol. 2011; 55: 369-78.
24. Kunzmann S, Collins JJ, Yang Y, et al. Antenatal inflammation reduces Cav-1 expression and influences multiple signaling pathways in preterm foetal lungs. Am J Respir Cell Mol Biol. 2011; 45: 969-76.
25. Hartung A, Bitton-Worms K, Rechtman MM, et al. Different routes of bone morphogenic protein (BMP) receptor endocytosis influence BMP signaling. Mol Cell Biol. 2006; 26: 7791-805.
26. Nakerakanti SS, Bujor AM, Trojanowska M. CCN2 is required for the TGF-beta induced activation of Smad1-Erk1/2 signaling network. PLoS One. 2011; 6: e21911.
27. Bujor AM, Nakerakanti S, Morris E, et al. Akt inhibition up-regulates MMP1 through a CCN2-dependent pathway in human dermal fibroblasts. Exp Dermatol. 2010; 19: 347-54.