Characterization of the novel xylanase from the thermophilic Geobacillus thermodenitrificans JK1

Microbiology (Russian Federation)

Volumn 81, Issue 4, 2012, Pages 418-424

  Gerasimova, J ^a   Kuisiene, N ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

Author keywords

endospore forming; G. thermodenitrificans; Geobacillus; low thermostability; thermostable xylanase; xylan

Indexed keywords

EID: 84865431491     PISSN: 00262617     EISSN: None     Source Type: Journal    
DOI: 10.1134/S0026261712040066     Document Type: Article

References (21)

1. Zhang, Z.-G., Yi, Z.-L., Pei, X.-Q., and Wu, Z.-L., Improving the Thermostability of Geobacillus stearothermophilus Xylanase XT6 by Directed Evolution and Site-Directed Mutagenesis, Biores. Tech., 2010, vol. 101, pp. 9272-9278.
2. Sharma, A., Adhikari, S., and Satyanarayana, T., Alkali-Thermostable and Cellulase-Free Xylanase Production by an Extreme Thermophile Geobacillus thermoleovorans, World J. Microbiol. Biotechnol., 2007, vol. 23, pp. 483-490.
3. Khasin, A., Alchanati, I., and Shoham, Y., Purification and Characterization of a Thermostable Xylanase from Bacillus stearothermophilus T-6, Appl. Environ. Microbiol., 1993, vol. 59, pp. 1725-1730.
4. Baba, T., Shinke, R., and Nanmori, T., Identification and Characterization of Clustered Genes for Thermostable Xylan-Degrading Enzymes, β-Xylosidase and Xylanase, of Bacillus stearothermophilus 21, Appl. Environ. Microbiol., 1994, vol. 60, pp. 2252-2258.
5. Saksono, B. and Sukmarini, L., Structural Analysis of Xylanase from Marine Thermophilic Geobacillus stearothermophilus in Tanjung Api, Poso, Indonesia, HAYATI J. Biosci., 2010, vol. 17, pp. 189-197.
6. Wu, S., Liu, B., and Zhang, X., Characterization of a Recombinant Thermostable Xylanase from Deep-Sea Thermophilic Geobacillus sp. MT-1 in East Pacific, Appl. Microbiol. Biotechnol., 2006, vol. 72, pp. 1210-1216.
7. Canakci, S., Inan, K., Kacagan, M., and Belduz, A. O., Evaluation of Arabinofuranosidase and Xylanase Activities of Geobacillus spp. Isolated from Some Hot Springs in Turkey, J. Microbiol. Biotechnol., 2007, vol. 17, pp. 1262-1270.
8. Kumar, R., Singh, S., and Singh, O., Bioconversion of Lignocellulosic Biomass: Biochemical and Molecular Perspectives, J. Ind. Microbiol. Biotechnol., 2008, vol. 35, pp. 377-391.
9. Lapidot, A., Mechaly, A., and Shoham, Y., Overexpression and Single-Step Purification of a Thermostable Xylanase from Bacillus stearothermophilus T-6, J. Biotechnol., 1996, vol. 51, pp. 259-264.
10. Kuisiene, N., Jomantiene, R., Valiunas, D., and Chitavichius, D., Characterization of Thermophilic Proteolytic Spore-Forming Bacteria from a Geothermal Site in Lithuania Based on 16S rDNA RFLP and ITS-PCR Analyses, Microbiology, 2002, vol. 71, pp. 712-716.
11. Kuisiene, N., Raugalas, J., and Chitavichius, D., Phylogenetic, Inter, and Intraspecific Sequence Analysis of spo0A Gene of the Genus Geobacillus, Curr. Microbiol., 2009, vol. 58, pp. 547-553.
12. Tamura, K., Dudley, J., Nei, M., and Kumar, S., MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4. 0, Mol. Biol. Evol., 2007, vol. 24, pp. 1596-1599.
13. Saitou, N. and Nei, M., The Neighbour-Joining Method: A New Method for Reconstructing Phylogenetic Trees, Mol. Biol. Evol., 1987, vol. 4, pp. 406-425.
14. Beg, Q. K., Kapoor, M., Mahajan, L., and Hoondal, G. S., Microbial Xylanases and Their Industrial Applications: A Review, Appl. Microbiol. Biotechnol., 2001, vol. 56, pp. 326-338.
15. Ng, I.-S., Li, C.-W., Yeh, Y.-F., Chen, P. T., Chir, J.-L., Ma, C.-H., Yu, S.-M., Ho, T. D., and Tong, C.-G., A Novel Endo-Glucanase from the Thermophilic Bacterium Geobacillus sp. 70PC53 with High Activity and Stability over a Broad Range of Temperatures, Extremophiles, 2009, vol. 13, pp. 425-435.
16. Bradford, M. M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248-254.
17. Laemmli, U. K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680-685.
18. Miller, G. L., Use of Dinitrosalicylic Reagent for Determination of Reducing Sugars, Anal. Chem., 1959, vol. 31, pp. 426-428.
19. Gat, O., Lapidot, A., Alchanati, I., Regueros, C., and Shoham, Y., Cloning and DNA Sequence of the Gene Coding for Bacillus stearothermophilus T-6 Xylanase, Appl. Environ. Microbiol., 1994, vol. 60, pp. 1889-1896.
20. Cantarel, B. L., Coutinho, P. M., Rancurel, C., Bernard, T., Lombard, V., and Henrissat, B., The Carbohydrate-Active EnZymes Database (CAZy): An Expert Resource for Glycogenomics, Nucleic Acids Res., 2009, vol. 37, pp. D233-238.
21. Subramaniyan, S. and Prema, P., Cellulase-Free Xylanases from Bacillus and Other Microorganisms, FEMS Microbiol. Lett., 2000, vol. 183, pp. 1-7.