Immobilization of enterokinase on magnetic supports for the cleavage of fusion proteins

Journal of Biotechnology

Volumn 161, Issue 3, 2012, Pages 378-382

  Santana, Sara D F ^a   Pina, Ana S ^a   Roque, Ana C A ^a  

^a UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

2NA; Dex; EDC; EK; Enterokinase; Enzymatic cleavage; Fusion protein; Ga; GD4K 2NA; Immobilization; Magnetic nanoparticles; MNP_Dex; MNP_Ga; MNPs; NHS; Sulfo SMCC

Indexed keywords

2NA; DEX; EDC; EK; ENTEROKINASE; ENZYMATIC CLEAVAGE; FUSION PROTEINS; MAGNETIC NANOPARTICLES; MNP_DEX; MNP_GA; MNPS; NHS; SULFO-SMCC;

BIOPOLYMERS; GALLIUM; IRON OXIDES; RADIOACTIVE WASTE VITRIFICATION;

NANOPARTICLES;

BIOPOLYMER; CYANAMIDE; ENTEROPEPTIDASE; HYBRID PROTEIN; IRON OXIDE; MAGNETIC NANOPARTICLE; MALEIMIDE;

ARTICLE; BIOCATALYST; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME ISOLATION; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN STRUCTURE;

CATALYTIC DOMAIN; ENTEROPEPTIDASE; ENZYMES, IMMOBILIZED; HYDRODYNAMICS; HYDROGEN-ION CONCENTRATION; MAGNETICS; MODELS, MOLECULAR; NANOPARTICLES; RECOMBINANT FUSION PROTEINS; STATIC ELECTRICITY; SUBSTRATE SPECIFICITY;

EID: 84865430068     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.07.005     Document Type: Article

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