Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of mucin 1

Biotechnology Journal

Volumn 4, Issue 11, 2009, Pages 1610-1618

  Kubitzki, Tina ^a   Minör, Daniel ^a   Mackfeld, Ursula ^a   Oldiges, Marco ^a   Noll, Thomas ^b   Lütz, Stephan ^a,c  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b BIELEFELD UNIVERSITY   (Germany)

^c NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Enterokinase; Fusion protein cleavage; Immobilization; MUC1; Protein purification

Indexed keywords

AMINO ACID SEQUENCE; BIOCATALYTIC PROCESS; CANCER IMMUNOTHERAPY; CHO CELL; ENZYME INACTIVATION; FUSION PROTEIN CLEAVAGE; FUSION PROTEINS; HIGH SPECIFICITY; PEPTIDE BONDS; POTENTIAL TOOL; PROCESS CONDITION; PROCESS TIME; PROTEIN PURIFICATION; PURIFICATION PROCESS; REACTION CYCLES; SERINE PROTEASE; TARGET PROTEINS; TOTAL TURNOVER;

AMINO ACIDS; BIOASSAY; BIOCHIPS; ENZYMES; MAMMALS; ORGANIC ACIDS; PHOSPHATASES; PURIFICATION;

ENZYME IMMOBILIZATION;

ENTEROPEPTIDASE; HYBRID PROTEIN; MUCIN 1;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CELL DISRUPTION; CHO CELL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME ISOLATION; ENZYME PURIFICATION; FERMENTATION; FREEZE DRYING; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN CLEAVAGE;

ANIMALS; CATTLE; CHO CELLS; CRICETINAE; CRICETULUS; ENTEROPEPTIDASE; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MAGNESIUM; MUCIN-1; RECOMBINANT FUSION PROTEINS;

BOVINAE; MAMMALIA;

EID: 73449148003     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200900049     Document Type: Article

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