Localizing flexible regions in proteins using hydrogen-deuterium exchange mass spectrometry

Methods in Molecular Biology

Volumn 896, Issue , 2012, Pages 375-385

  Bobst, Cedric E ^a   Kaltashov, Igor A ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Backbone protection; Flexible loop; Hydrogen deuterium exchange; Mass spectrometry; Protein conformation

Indexed keywords

GLUCOSYLCERAMIDASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE;

AMINO ACID SEQUENCE; DEUTERIUM EXCHANGE MEASUREMENT; GLUCOSYLCERAMIDASE; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY;

EID: 84865322358     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4614-3704-8_25     Document Type: Article

References (11)

1. Smith DL, Deng Y, Zhang Z (1997) Probing the non-covalent structure of proteins by amide hydrogen exchange and mass spectrometry. J Mass Spectrom 32:135-146
2. Zhang Z, Smith DL (1993) Determination of amide hydrogen exchange by mass spectrometry: a new tool for protein structure elucidation. Protein Sci 2:522-531
3. Bobst CE, Thomas JJ, Salinas PA, Savickas P, Kaltashov IA (2010) Impact of oxidation on protein therapeutics: conformational dynamics of intact and oxidized acid-beta-glucocerebrosidase at near-physiological pH. Protein Sci 19:2366-2378
4. Kaplan W, Littlejohn TG (2001) Swiss-PDB Viewer (Deep View). Brief Bioinform 2:195-197
5. Pascal BD, Chalmers MJ, Busby SA, Griffin PR (2009)HD desktop: an integrated platform for the analysis and visualization of H/D exchange data. J Am Soc Mass Spectrom 20:601-610
6. SlyszGW, Baker CA, Bozsa BM, Dang A, Percy AJ, Bennett M, Schriemer DC (2009) Hydra: software for tailored processing of H/D exchange data from MS or tandem MS analyses. BMC Bioinformatics 10:162
7. Weis DD, Engen JR, Kass IJ (2006) Semiautomated data processing of hydrogen exchange mass spectra using HX-Express. J Am Soc Mass Spectrom 17:1700-1703
8. Perkins DN, Pappin DJC, Creasy DM, Cottrell JS (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20:3551-3567
9. Clauser KR, Baker P, Burlingame AL (1999) Role of accurate mass measurement ( +-10 ppm) in protein identification strategies employing MS or MS MS and database searching. Anal Chem 71:2871-2882
10. Wang L, Pan H, Smith DL (2002) Hydrogen exchange-mass spectrometry: optimization of digestion conditions. Mol Cell Proteomics 1:132-138
11. Bai Y, Milne JS, Mayne L, Englander SW (1993) Primary structure effects on peptide group hydrogen exchange. Proteins 17:75-86