High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  Basters, Anja ^a   Ketscher, Lars ^a   Deuerling, Elke ^b   Arkona, Christoph ^c   Rademann, Jörg ^c   Knobeloch, Klaus Peter ^a   Fritz, Günter ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF KONSTANZ   (Germany)

^c UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE PROTEINS; ADDUCT FORMATION; COVALENT LINKAGE; E. COLI; ENZYMATIC ACTIVITIES; FUSION PROTEINS; FUSION SYSTEMS; HETEROLOGOUS EXPRESSION; HIGH YIELD; HIGH-THROUGHPUT; PROTEASE INHIBITION; SINGLE-STEP; SMALL MOLECULE INHIBITOR; STRUCTURAL CHARACTERIZATION; TARGET PROTEINS; TRIGGER FACTORS; UBIQUITIN; UBIQUITIN-LIKE PROTEINS; VINYL SULFONES; VIRAL INFECTIONS; VIRAL REPLICATION;

ESCHERICHIA COLI;

PROTEINS;

HYBRID PROTEIN; SULFONE; UBIQUITIN; UNCLASSIFIED DRUG; USP18 PROTEIN; VINYL DERIVATIVE;

ARTICLE; CONJUGATION; COVALENT BOND; ENZYME ACTIVITY; HYDROLYSIS; NONHUMAN; PROTEIN EXPRESSION;

ANIMALS; ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FIBROBLASTS; GENE EXPRESSION; MICE; PEPTIDYLPROLYL ISOMERASE; PROTEIN FOLDING; RECOMBINANT PROTEINS;

EID: 84865239203     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-56     Document Type: Article

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