Trichosanthin inhibits DNA methyltransferase and restores methylation-silenced gene expression in human cervical cancer cells

Molecular Medicine Reports

Volumn 6, Issue 4, 2012, Pages 872-878

  Huang, Yiling ^a,b   Song, Huamei ^b   Hu, Huojun ^b   Cui, Lei ^a,b   You, Chengcheng ^b   Huang, Liming ^a  

^a WUHAN UNIVERSITY   (China)

^b CHINA THREE GORGES UNIVERSITY   (China)

Author keywords

Cervical cancer; DNA methylation; Trichosanthin

Indexed keywords

DNA METHYLTRANSFERASE 1; MESSENGER RNA; TRICHOSANTHIN; TUMOR SUPPRESSOR IN LUNG CANCER 1; TUMOR SUPPRESSOR PROTEIN; UNCLASSIFIED DRUG;

ADENOMATOUS POLYP; ARTICLE; CANCER CELL; CANCER CELL CULTURE; CONTROLLED STUDY; CPG ISLAND; DEMETHYLATION; DNA METHYLATION; DOSE RESPONSE; ENZYME ACTIVITY; ENZYME INHIBITION; GENE EXPRESSION; GENE SILENCING; HELA CELL; HUMAN; HUMAN CELL; PROMOTER REGION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SQUAMOUS CELL CARCINOMA; TUMOR SUPPRESSOR GENE; UTERINE CERVIX CANCER; WESTERN BLOTTING;

ADENOMATOUS POLYPOSIS COLI PROTEIN; ANTINEOPLASTIC AGENTS, PHYTOGENIC; CELL ADHESION MOLECULES; CELL LINE, TUMOR; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DNA METHYLATION; FEMALE; HELA CELLS; HUMANS; IMMUNOGLOBULINS; TRICHOSANTHIN; UTERINE CERVICAL NEOPLASMS;

EID: 84865170990     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2012.994     Document Type: Article

References (29)

1. Narayan G and Murty VV: Integrative genomic approaches in cervical cancer: implications for molecular pathogenesis. Future Oncol 6: 1643-1652, 2010.
2. Feng Q, Balasubramanian A, Hawes SE, et al: Detection of hypermethylated genes in women with and without cervical neoplasia. J Natl Cancer Inst 97: 273-282, 2005. (Pubitemid 40310275)
3. Jurkowska RZ, Jurkowski TP and Jeltsch A: Structure and function of mammalian DNA methyltransferases. Chembiochem 12: 206-222, 2011.
4. Turek-Plewa J and Jagodziński PP: The role of mammalian DNA methyltransferases in the regulation of gene expression. Cell Mol Biol Lett 10: 631-647, 2005. (Pubitemid 43161688)
5. Hermann A, Goyal R and Jeltsch A: The Dnmt1 DNA-(cytosine-C5)- methyltransferase methylates DNA processively with high preference for hemimethylated target sites. J Biol Chem 279: 48350-48359, 2004. (Pubitemid 39540991)
6. Ren J, Singh BN, Huang Q, et al: DNA hypermethylation as a chemotherapy target. Cell Signal 23: 1082-1093, 2011.
7. Juttermann R, Li E and Jaenisch R: Toxicity of 5-aza-2′- deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation. Proc Natl Acad Sci USA 91: 11797-11801, 1994.
8. Cheng JC, Matsen CB, Gonzales FA, et al: Inhibition of DNA methylation and reactivation of silenced genes by zebularine. J Natl Cancer Inst 95: 399-409, 2003. (Pubitemid 36395260)
9. Dueñas-González A, Lizano M, Candelaria M, et al: Epigenetics of cervical cancer. An overview and therapeutic perspectives. Mol Cancer 4: 38, 2005.
10. Collins EJ, Robertus JD, LoPresti M, et al: Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin. J Biol Chem 265: 8665-8669, 1990.
11. Pan KZ, Lin YJ, Zhou KJ, et al: The crystal and molecular structure of trichosanthin at 2.6 A resolution. Sci China B 36: 1069-1081, 1993.
12. Li M, Li X and Li JC: Possible mechanisms of trichosanthin-induced apoptosis of tumor cells. Anat Rec (Hoboken) 293: 986-992, 2010.
13. Ding BN, Chen DJ, Li XR et al: Trichosanthin inhibits growth of breast cancer cells in vitro and in vivo. J Pract Oncol 23: 310-313, 2008.
14. Li J, Xia X, Nie H, et al: PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562. Biochim Biophys Acta 1770: 63-70, 2007. (Pubitemid 44920351)
15. Li J, Xia X, Ke Y, et al: Trichosanthin induced apoptosis in HL-60 cells via mitochondrial and endoplasmic reticulum stress signaling pathways. Biochim Biophys Acta 1770: 1169-1180, 2007. (Pubitemid 47009016)
16. Zhang CY, An CC, Wang RY, et al: Capillary electrophoresis and circular dichroism study of trichosanthin and its mutants. Talanta 57: 467-473, 2002.
17. Xie L, Wei J, Qian X, et al: CXCR4, a potential predictive marker for docetaxel sensitivity in gastric cancer. Anticancer Res 30: 2209-2216, 2010.
18. Steenbergen RD, Kramer D, Braakhuis BJ, et al: TSLC1 gene silencing in cervical cancer cell lines and cervical neoplasia. J Natl Cancer Inst 96: 294-305, 2004. (Pubitemid 38256274)
19. Minami K, Chano T, Kawakami T, et al: DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma. Clin Cancer Res 16: 2751-2759, 2010.
20. Arnold CN, Goel A, Niedzwiecki D, et al: APC promoter hypermethylation contributes to the loss of APC expression in colorectal cancers with allelic loss on 5q. Cancer Biol Ther 3: 960-964, 2004. (Pubitemid 41351019)
21. Murray PG, Fan Y, Davies G, et al: Epigenetic silencing of a proapoptotic cell adhesion molecule, the immunoglobulin super-family member IGSF4, by promoter CpG methylation protects Hodgkin lymphoma cells from apoptosis. Am J Pathol 177: 1480-1490, 2010.
22. Jemal A, Siegel R, Ward E, et al: Cancer statistics. CA Cancer J Clin 59: 225-249, 2009.
23. Sasieni P and Adams J: Changing rates of adenocarcinoma and adenosquamous carcinoma of the cervix in England. Lancet 357: 1490-1493, 2001. (Pubitemid 32488859)
24. Kang S, Kim JW, Kang GH, et al: Comparison of DNA hypermethylation patterns in different types of uterine cancer: cervical squamous cell carcinoma, cervical adenocarcinoma and endometrial adenocarcinoma. Int J Cancer 118: 2168-2171, 2006.
25. Ying Y and Tao Q: Epigenetic disruption of the WNT/betacatenin signaling pathway in human cancers. Epigenetics 4: 307-312, 2009.
26. Wentzensen N, Sherman ME, Schiffman M and Wang SS: Utility of methylation markers in cervical cancer early detection: appraisal of the state-of-the-science. Gynecol Oncol 112: 293-299, 2009.
27. Wu S, Lu XH, Zhu YR, et al: N-glycosidase mechanism of Trichosanthin. Sci China C Life Sci 41: 174-180, 1998. (Pubitemid 128492160)
28. Nie H, Cai X, He X, et al: Position 120-123, a potential active site of trichosanthin. Life Sci 62: 491-500, 1998. (Pubitemid 28050067)
29. Huang J, Plass C and Gerhauser C: Cancer chemoprevention by targeting the epigenome. Curr Drug Targets 12: 1925-1956, 2011.