siRNAs trigger efficient silencing of a parasitism gene in plant parasitic root-knot nematodes

Genes

Volumn 3, Issue 3, 2012, Pages 391-408

  Arguel, Marie Jeanne ^a,b,c   Jaouannet, Maëlle ^a,b,c   Magliano, Marc ^a,b,c   Abad, Pierre ^a,b,c   Rosso, Marie Noëlle ^a,b,c,d  

^a Institut Sophia Agrobiotech   (France)

^b UNIVERSITÉ CÔTE D'AZUR   (France)

^c CNRS   (France)

^d AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Meloidogyne incognita; Plant parasitic nematode; RKN; RNA interference; RNAi; Root knot nematode; Short interfering RNA; siRNA

Indexed keywords

ACONITATE HYDRATASE; ACTIN; ACYL COENZYME A DEHYDROGENASE; CALRETICULIN; CITRATE SYNTHASE; COMPLEMENTARY DNA; DOUBLE STRANDED RNA; GEX INTERACTING PROTEIN 7; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCEROL KINASE; PYRUVATE KINASE; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; DNA BASE COMPOSITION; ENDOCYTOSIS; ESOPHAGUS; FATTY ACID METABOLISM; FATTY ACID OXIDATION; GENE; GENE DOSAGE; GENE EXPRESSION; GENE LOCATION; GENE SILENCING; GLUCONEOGENESIS; GLYCOLYSIS; MELOIDOGYNE INCOGNITA; MI CRT GENE; NEMATODE; NONHUMAN; PARASITE VIABILITY; PARASITE VIRULENCE; PARASITISM; PHENOTYPE; PLANT PARASITIC PLANT DISEASE; POLYMERASE CHAIN REACTION; REVERSE GENETICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA INTERFERENCE; RNA STRUCTURE; UPREGULATION;

EID: 84865090093     PISSN: None     EISSN: 20734425     Source Type: Journal    
DOI: 10.3390/genes3030391     Document Type: Article

References (35)

1. Abad, P.; Favery, B.; Rosso, M.-N.; Castagnone-Sereno, P. Root-knot nematode parasitism and host response: Molecular basis of a sophisticated interaction. Mol. Plant Pathol. 2003, 4, 217-224.
2. Djian-Caporalino, C. Root-knot nematodes (meloidogyne spp.), a growing problem in french vegetable crops. OEPP/EPPO Bull. 2012, 42, 1-12.
3. Blok, V.C.; Jones, J.T.; Phillips, M.S.; Trudgill, D.L. Parasitism genes and host range disparities in biotrophic nematodes: The conundrum of polyphagy versus specialisation. BioEssays 2008, 30, 249-259.
4. Rosso, M.-N.; Hussey, R.S.; Davis, E.L.; Smant, G.; Baum, T.J.; Abad, P.; Mitchum, M.G. Nematode Effector Proteins: Targets and Functions in Plant Parasitism. In Effectors in Plant-Microbe Interactions; Wiley-Blackwell: Hoboken, NJ, USA, 2011; pp. 327-354.
5. Rosso, M.N.; Jones, J.T.; Abad, P. Rnai and Functional genomics in plant parasitic nematodes. In Annual Review of Phytopathology; Annual Reviews: Palo Alto, CA, USA, 2009; Volume 47, pp. 207-232.
6. Gheysen, G.; Vanholme, B. Rnai from plants to nematodes. Trends Biotechnol. 2007, 25, 89-92.
7. Lilley, C.J.; Davies, L.J.; Urwin, P.E. Rna interference in plant parasitic nematodes: A summary of the current status. Parasitology 2012, 139, 1-11.
8. Mello, C.C.; Conte, D. Revealing the world of rna interference. Nature 2004, 431, 338-342.
9. Sijen, T.; Fleenor, J.; Simmer, F.; Thijssen, K.L.; Parrish, S.; Timmons, L.; Plasterk, R.H.A.; Fire, A. On the Role of RNA Amplification in dsRNA-Triggered Gene Silencing. Cell 2001, 107, 465-476.
10. Abad, P.; Gouzy, J.; Aury, J.M.; Castagnone-Sereno, P.; Danchin, E.G.J.; Deleury, E.; Perfus-Barbeoch, L.; Anthouard, V.; Artiguenave, F.; Blok, V.C.; et al. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita. Nature Biotechnology 2008, 26, 909-915.
11. Dalzell, J.J.; McVeigh, P.; Warnock, N.D.; Mitreva, M.; Bird, D.M.; Abad, P.; Fleming, C.C.; Day, T.A.; Mousley, A.; Marks, N.J.; Maule, A.G. RNAi Effector Diversity in Nematodes. Plos Neglected Tropical Diseases 2011, 5.
12. Dalzell, J.J.; Warnock, N.D.; Stevenson, M.A.; Mousley, A.; Fleming, C.C.; Maule, A.G. Short interfering RNA-mediated knockdown of drosha and pasha in undifferentiated Meloidogyne incognita eggs leads to irregular growth and embryonic lethality. International Journal for Parasitology 2010, 40, 1303-1310.
13. Huang, G.Z.; Allen, R.; Davis, E.L.; Baum, T.J.; Hussey, R.S. Engineering broad root-knot resistance in transgenic plants by rnai silencing of a conserved and essential root-knot nematode parasitism gene. Proc. Natl. Acad. Sci. USA 2006, 103, 14302-14306.
14. Yadav, B.C.; Veluthambi, K.; Subramaniam, K. Host-generated double stranded rna induces rnai in plant-parasitic nematodes and protects the host from infection. Mol. Biochem. Parasitol. 2006, 148, 219-222.
15. Fairbairn, D.J.; Cavallaro, A.S.; Bernard, M.; Mahalinga-Iyer, J.; Graham, M.W.; Botella, J.R. Host-delivered rnai: An effective strategy to silence genes in plant parasitic nematodes. Planta 2007, 226, 1525-1533.
16. Dubreuil, G.; Magliano, M.; Dubrana, M.P.; Lozano, J.; Lecomte, P.; Favery, B.; Abad, P.; Rosso, M.N. Tobacco rattle virus mediates gene silencing in a plant parasitic root-knot nematode. J. Exp. Bot. 2009, 60, 4041-4050.
17. Valentine, T.A.; Randall, E.; Wypijewski, K.; Chapman, S.; Jones, J.; Oparka, K.J. Delivery of macromolecules to plant parasitic nematodes using a tobacco rattle virus vector. Plant Biotechnol. J. 2007, 5, 827-834.
18. Dalzell, J.J.; McMaster, S.; Johnston, M.J.; Kerr, R.; Fleming, C.C.; Maule, A.G. Non-nematodederived double-stranded rnas induce profound phenotypic changes in meloidogyne incognita and globodera pallida infective juveniles. Int. J. Parasitol. 2009, 39, 1503-1516.
19. Dalzell, J.J.; McMaster, S.; Fleming, C.C.; Maule, A.G. Short interfering rna-mediated gene silencing in globodera pallida and meloidogyne incognita infective stage juveniles. Int. J. Parasitol. 2010, 40, 91-100.
20. Vieira, P.; Danchin, E.G.J.; Neveu, C.; Crozat, C.; Jaubert, S.; Hussey, R.S.; Engler, G.; Abad, P.; de Almeida-Engler, J.; Castagnone-Sereno, P.; et al. The plant apoplasm is an important recipient compartment for nematode secreted proteins. J. Exp. Bot. 2011, 62, 1241-1253.
21. Luedtke, S.; O'Connor, V.; Holden-Dye, L.; Walker, R.J. The regulation of feeding and metabolism in response to food deprivation in caenorhabditis elegans. Invertebr. Neurosci. 2010, 10, 63-76
22. Zhao, M.; Yang, H.; Jiang, X.J.; Zhou, W.; Zhu, B.; Zeng, Y.; Yao, K.T.; Ren, C.P. Lipofectamine rnaimax: An efficient sirna transfection reagent in human embryonic stem cells. Mol. Biotechnol. 2008, 40, 19-26.
23. Meloidogyne Incognita Resource. Available online: http://www.inra.fr/meloidogyne_incognita/(Accessed on January 2011).
24. RNAfold WebServer. Available online: http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cg (Accessed on January 2011).
25. Melillo, M.T.; Leonetti, P.; Bongiovanni, M.; Castagnone-Sereno, P.; Bleve-Zacheo, T. Modulation of reactive oxygen species activities and h2o2 accumulation during compatible and incompatible tomato-root-knot nematode interactions. New Phytol. 2006, 170, 501-512.
26. Inoh, Y.; Furuno, T.; Hirashima, N.; Kitamoto, D.; Nakanishi, M. Rapid delivery of small interfering rna by biosurfactant mel-a-containing liposomes. Biochem. Biophys. Res. Commun. 2011, 414, 635-640.
27. Dubreuil, G.; Magliano, M.; Deleury, E.; Abad, P.; Rosso, M.N. Transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism. New Phytol. 2007, 176, 426-436.
28. Bakhetia, M.; Urwin, P.E.; Atkinson, H.J. Characterisation by rnai of pioneer genes expressed in the dorsal pharyngeal gland cell of heterodera glycines and the effects of combinatorial rnai. Int. J. Parasitol. 2008, 38, 1589-1597.
29. Bakhetia, M.; Urwin, P.E.; Atkinson, H.J. Qpcr analysis and rnai define pharyngeal gland cell-expressed genes of heterodera glycines required for initial interactions with the host. Mol. Plant Microbe Interact 2007, 20, 306-312.
30. Jaubert, S.; Milac, A.L.; Petrescu, A.J.; de Almelda-Engler, J.; Abad, P.; Rosso, M.N. In planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode. Mol. Plant Microbe Interact. 2005, 18, 1277-1284.
31. 2-1,4-endoglucanase in the root-knot nematode meloidogyne incognita and expression analysis during plant parasitism. Mol. Plant Microbe Interact. 1999, 12, 585-591.
32. Chomczynski, P.; Sacchi, N. The single-step method of rna isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on. Nat. Protoc. 2006, 1, 581-585.
33. Pfaffl, M.W. A new mathematical model for relative quantification in real-time rt-pcr. Nucleic Acids Res. 2001, 29, E45.
34. Hong, J.; Qian, Z.K.; Shen, S.Y.; Min, T.S.; Tan, C.; Xu, J.F.; Zhao, Y.C.; Huang, W.D. High doses of sirnas induce eri-1 and adar-1 gene expression and reduce the efficiency of rna interference in the mouse. Biochem. J. 2005, 390, 675-679.
35. Melnyk, C.W.; Molnar, A.; Baulcombe, D.C. Intercellular and systemic movement of rna silencing signals. EMBO J. 2011, 30, 3553-3563.