Expression, purification, and characterization of recombinant purine nucleoside phosphorylase from Escherichia coli

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 180-188

  Lee, John ^a   Filosa, Serena ^a   Bonvin, Julie ^a   Guyon, Sebastien ^a   Aponte, Raphael A ^a   Turnbull, Joanne L ^a  

^a Gina Cody School of Engineering and Computer Science   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYST ACTIVITY; ENZYME ASSAY; EXPRESSION VECTOR; GENE INSERTION; GENE OVEREXPRESSION; GENOMIC FRAGMENT; INOSINE; ISOPROPYL BETA D THIOGALACTOSIDE; PHOSPHATE; PROMOTER REGION; PROTEIN PURIFICATION; PURINE NUCLEOSIDE PHOSPHORYLASE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; STEADY STATE; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034964053     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1437     Document Type: Article

References (27)

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2. The crystal structure of Escherichia coli purine nucleoside phosphorylase: A comparison with the human enzyme reveals a conserved topology
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4. Drugs by design
5. Tumor cell bystander killing in colonic carcinoma utilizing the Escherichia coli deoD gene to generate toxic purines
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7. Purine nucleoside synthesis, an efficient method employing nucleoside phosphorylases
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12. A continuous visible spectrophotometric assay for aspartate transcarbamylase
13. A continuous spectrophotometric assay for aspartate transcarbamylase and ATPase
14. Crystal structure of the purine nucleoside phosphorylase (PNP) from Cellulomonas sp., and its implication for the mechanism of trimeric PNPs
15. Formycins A and B and some analogues: Selective inhibitors of bacterial (Escherichia coli) purine nucleoside phosphorylase
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17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
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20. Crystallization and preliminary X-ray investigation of purine nucleoside phosphorylase from Escherichia coli
21. Use of site directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol
22. Studies of inhibitor binding to Escherichia coli purine nucleoside phosphorylase using the transferred nuclear effect and rotating-frame nuclear overhauser enhancement
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24. Synthesis and PMR studies of some methylated 6-thiopurine nucleosides