Preparation by differential gradient centrifugation is better than swim- up in selecting sperm with normal morphology (strict criteria)

Fertility and Sterility

Volumn 69, Issue 4, 1998, Pages 722-726

  Prakash, Pranav ^a   Leykin, Lucy ^a   Chen, Zuying ^b   Toth, Thomas ^b   Sayegh, Raja ^a   Schiff, Isaac ^a   Isaacson, Keith ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b NONE

Author keywords

Morphology; Percoll; Sperm preparation; Swim up

Indexed keywords

ARTICLE; CELL STRUCTURE; FERTILIZATION; FERTILIZATION IN VITRO; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERMATOZOON MOTILITY;

EID: 0031969404     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(98)00002-8     Document Type: Article

References (18)

1. Mortimer D, Leslie EE, Kelly RW, Templeton AA. Morphological selection of human spermatozoa in vivo and in vitro. J Reprod Fertil. 64:1982;391-399.
2. Menkeveld R, Swanson RJ, Kotze TJvW, Kruger TF. Comparison of a discontinuous Percoll gradient method versus a swim-up method effects of sperm morphology and other semen parameters . Andrologia. 22:1990;152-158.
3. Liu DY, DuPlessis YP, Nayudu PL, Johnston WI, Baker HWG. The use of in vitro fertilization to evaluate putative tests of human sperm function. Fertil Steril. 49:1988;272-277.
4. Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehninger S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril. 49:1988;112-117.
5. Enginsu ME, Dumoulin JCM, Pieters MHEC, Evers JLH, Geraedts JPM. Predictive value of morphologically normal sperm concentration in the medium for in vitro fertilization. Int J Androl. 16:1993;113-120.
6. Yang YS, Chen SU, Ho HN, Chen HF, Chao KH, Lin HR, et al. Correlation between sperm morphology using strict criteria in original semen and swim-up inseminate and human in vitro fertilization. Arch Androl. 34:1995;105-113.
7. Grow DR, Oehninger S, Seltman HJ, Toner JP, Swanson JR, Kruger TF, et al. Sperm morphology as diagnosed by strict criteria probing the impact of teratozoospermia on fertilization rate and pregnancy outcome in a large in vitro fertilization population . Fertil Steril. 62:1994;559-567.
8. Hinting A, Comhaire F, Vermeulen L, Dhout M, Vermeulen A, Vanderckhove D. Value of sperm characteristics and the result of in vitro fertilization for predicting the outcome of assisted reproduction. Int J Androl. 13:1990;59-66.
9. Kruger TF, Menkeveld R, Stander FSH, Lombard CJ, Van der Merwe JP, vanZyl JA, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 46:1986;1118-1123.
10. Van Der Zwalmen P, Bertin-Segal G, Geerts L, Debauche C, Schoysman R. Sperm morphology and IVF pregnancy rate comparison between Percoll gradient centrifugation and swim-up procedures . Hum Reprod. 6:1991;581-588.
11. Englert Y, Van denBergh M, Rodesch C, Bertrand E, Biramane E, Legreve A. A comparative auto-controlled study between swim-up and Percoll preparation of fresh semen samples for in-vitro fertilization. Hum Reprod. 7:1992;399-402.
12. Smith S, Hosid S, Scott L. Use of postseparation sperm parameters to determine the method of choice for sperm preparation for assisted reproductive technology. Fertil Steril. 63:1995;591-597.
13. Le Lannau D, Blanchard Y. Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or swim-up procedure. J Reprod Fertil. 84:1988;551-556.
14. Pousette A, Akerlof E, Rosenberg L, Fredricsson B. Increase in progressive motility and improved morphology of human spermatozoa following their migration through Percoll gradients. Int J Androl. 9:1983;1-13.
15. Aitken RJ. Assessment of sperm function for IVF. Hum Reprod. 3:1988;89-95.
16. Guerin JF, Mathieu C, Lorange J, Pinatel MC, Boulieu D. Improvement of survival and fertilizing capacity of human spermatozoa in an IVF programme by selection on discontinuous Percoll gradients. Hum Reprod. 4:1989;798-804.
17. Berger T, Marrs RP, Moyer DL. Comparison of techniques for selection of motile spermatozoa. Fertil Steril. 43:1985;268-273.
18. Tanphaichitr N, Millette A, Agulnick A, Fitzgerald LM. Egg penetration ability and structural properties of human sperm prepared by Percoll-gradient centrifugation. Gamete Research. 20:1988;67-81.