Loss of meningococcal PilU delays microcolony formation and attenuates virulence in vivo

Infection and Immunity

Volumn 80, Issue 7, 2012, Pages 2538-2547

  Eriksson, Jens ^a   Eriksson, Olaspers Sara ^a   Jonsson, Ann Beth ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MENINGOCOCCAL PILT PROTEIN; MENINGOCOCCAL PILU PROTEIN; UNCLASSIFIED DRUG;

ANIMAL MODEL; ARTICLE; BACTEREMIA; BACTERIAL COUNT; BACTERIAL MICROCOLONY FORMATION; BACTERIAL SURVIVAL; BACTERIAL VIRULENCE; BACTERIUM MUTANT; COLONY FORMATION; EPITHELIUM CELL; GENE DELETION; HOST CELL; HUMAN; HUMAN CELL; MOUSE; NEISSERIA MENINGITIDIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SURVIVAL RATE; WILD TYPE;

ANIMALS; BACTERIAL ADHESION; BACTERIAL LOAD; DISEASE MODELS, ANIMAL; EPITHELIAL CELLS; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; GENE DELETION; HUMANS; MENINGOCOCCAL INFECTIONS; MICE; MICE, TRANSGENIC; NEISSERIA MENINGITIDIS; SURVIVAL ANALYSIS; VIRULENCE; VIRULENCE FACTORS;

EID: 84864815548     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.06354-11     Document Type: Article

References (34)

1. Aas FE, et al. 2006. Neisseria gonorrhoeae type IV pili undergo multisite, hierarchical modifications with phosphoethanolamine and phosphocholine requiring an enzyme structurally related to lipopolysaccharide phosphoethanolamine transferases. J. Biol. Chem. 281, 27712-27723.
2. Aas FE, Lovold C, Koomey M. 2002. An inhibitor of DNA binding and uptake events dictates the proficiency of genetic transformation in Neisseria gonorrhoeae, mechanism of action and links to type IV pilus expression. Mol. Microbiol. 46, 1441-1450.
3. Albiger B, Johansson L, Jonsson AB. 2003. Lipooligosaccharide-deficient Neisseria meningitidis shows altered pilus-associated characteristics. Infect. Immun. 71, 155-162.
4. Brown DR, Helaine S, Carbonnelle E, Pelicic V. 2010. Systematic functional analysis reveals that a set of seven genes is involved in fine-tuning of the multiple functions mediated by type IV pili in Neisseria meningitidis. Infect. Immun. 78, 3053-3063.
5. Chamot-Rooke J, et al. 2011. Posttranslational modification of pili upon cell contact triggers N. meningitidis dissemination. Science 331, 778-782.
6. Chiang P, et al. 2008. Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU. Microbiology 154, 114-126.
7. Comolli JC, et al. 1999. Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia. Infect. Immun. 67, 3625-3630.
8. Frasch CE, Mocca LF. 1978. Heat-modifiable outer membrane proteins of Neisseria meningitidis and their organization within the membrane. J. Bacteriol. 136, 1127-1134.
9. Han X, et al. 2008. Twitching motility is essential for virulence in Dichelobacter nodosus. J. Bacteriol. 190, 3323-3335.
10. Helaine S, et al. 2005. PilX, a pilus-associated protein essential for bacterial aggregation, is a key to pilus-facilitated attachment of Neisseria meningitidis to human cells. Mol. Microbiol. 55, 65-77.
11. Johansson L, et al. 2003. CD46 in meningococcal disease. Science 301, 373-375.
12. Johansson L, et al. 2005. Human-like immune responses in CD46 transgenic mice. J. Immunol. 175, 433-440.
13. Jones A, Georg M, Maudsdotter L, Jonsson AB. 2009. Endotoxin, capsule, and bacterial attachment contribute to Neisseria meningitidis resistance to the human antimicrobial peptide LL-37. J. Bacteriol. 191, 3861-3868.
14. Kuwae A, et al. 2011. NafA negatively controls Neisseria meningitidis piliation. PLoS One 6, e21749. doi, 10.1371/journal.pone.0021749.
15. Madico G, et al. 2006. The meningococcal vaccine candidate GNA1870 binds the complement regulatory protein factor H and enhances serum resistance. J. Immunol. 177, 501-510.
16. Merz AJ, So M, Sheetz MP. 2000. Pilus retraction powers bacterial twitching motility. Nature 407, 98-102.
17. Naessan CL, et al. 2008. Genetic and functional analyses of PptA, a phospho-form transferase targeting type IV pili in Neisseria gonorrhoeae. J. Bacteriol. 190, 387-400.
18. Nassif X, et al. 1994. Roles of pilin and PilC in adhesion of Neisseria meningitidis to human epithelial and endothelial cells. Proc. Natl. Acad. Sci. U. S. A. 91, 3769-3773.
19. Nassif X, et al. 1993. Antigenic variation of pilin regulates adhesion of Neisseria meningitidis to human epithelial cells. Mol. Microbiol. 8, 719-725.
20. Park HS, Wolfgang M, Koomey M. 2002. Modification of type IV pilusassociated epithelial cell adherence and multicellular behavior by the PilU protein of Neisseria gonorrhoeae. Infect. Immun. 70, 3891-3903.
21. Pelicic V. 2008. Type IV pili, e pluribus unum? Mol. Microbiol. 68, 827-837.
22. Pujol C, Eugene E, de Saint Martin L, Nassif X. 1997. Interaction of Neisseria meningitidis with a polarized monolayer of epithelial cells. Infect. Immun. 65, 4836-4842.
23. Pujol C, Eugene E, Marceau M, Nassif X. 1999. The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion. Proc. Natl. Acad. Sci. U. S. A. 96, 4017-4022.
24. Rahman M, Kallstrom H, Normark S, Jonsson AB. 1997. PilC of pathogenic Neisseria is associated with the bacterial cell surface. Mol. Microbiol. 25, 11-25.
25. Rudel T, et al. 1995. Role of pili and the phase-variable PilC protein in natural competence for transformation of Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. U. S. A. 92, 7986-7990.
26. Rudel T, Scheurerpflug I, Meyer TF. 1995. Neisseria PilC protein identified as type-4 pilus tip-located adhesin. Nature 373, 357-359.
27. Sjolinder H, Jonsson AB. 2007. Imaging of disease dynamics during meningococcal sepsis. PLoS One 2, e241. doi, 10.1371/journal.pone.0000241.
28. Tonjum T, Caugant DA, Dunham SA, Koomey M. 1998. Structure and function of repetitive sequence elements associated with a highly polymorphic domain of the Neisseria meningitidis PilQ protein. Mol. Microbiol. 29, 111-124.
29. Virji M, et al. 1991. The role of pili in the interactions of pathogenic Neisseria with cultured human endothelial cells. Mol. Microbiol. 5, 1831-1841.
30. Whitchurch CB, Mattick JS. 1994. Characterization of a gene, pilU, required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa. Mol. Microbiol. 13, 1079-1091.
31. Wolfgang M, et al. 1998. PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae. Mol. Microbiol. 29, 321-330.
32. Wolfgang M, Park HS, Hayes SF, van Putten JP, Koomey M. 1998. Suppression of an absolute defect in type IV pilus biogenesis by loss-offunction mutations in pilT, a twitching motility gene in Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. U. S. A. 95, 14973-14978.
33. Wolfgang M, van Putten JP, Hayes SF, Dorward D, Koomey M. 2000. Components and dynamics of fiber formation define a ubiquitous biogenesis pathway for bacterial pili. EMBO J. 19, 6408-6418.
34. Zolfaghar I, Evans DJ, Fleiszig SM. 2003. Twitching motility contributes to the role of pili in corneal infection caused by Pseudomonas aeruginosa. Infect. Immun. 71, 5389-5393.