Synthesis of O-serogroup specific positive controls and real-time PCR standards for nine clinically relevant non-O157 STECs

Journal of Microbiological Methods

Volumn 91, Issue 1, 2012, Pages 52-56

  Conrad, Cheyenne C ^a,b   Gilroyed, Brandon H ^c   McAllister, Tim A ^b   Reuter, Tim ^d  

^a UNIVERSITY OF LETHBRIDGE   (Canada)

^b LETHBRIDGE RESEARCH CENTRE   (Canada)

^c UNIVERSITY OF GUELPH   (Canada)

^d ALBERTA AGRICULTURE AND RURAL DEVELOPMENT   (Canada)

Author keywords

Detection; Multiplex; Real time PCR; STEC; Synthetic standards

Indexed keywords

OLIGONUCLEOTIDE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; DNA EXTRACTION; DNA FRAGMENTATION; DNA TEMPLATE; ESCHERICHIA COLI O157; GEL ELECTROPHORESIS; GENE AMPLIFICATION; HOST CELL; MOLECULAR CLONING; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEROTYPE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; TOXIN SYNTHESIS;

ESCHERICHIA COLI INFECTIONS; HUMANS; O ANTIGENS; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SHIGA-TOXIGENIC ESCHERICHIA COLI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84864815398     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.07.007     Document Type: Article

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