High accuracy of mesoscopic epi-fluorescence tomography for non-invasive quantitative volume determination of fluorescent protein-expressing tumours in mice

European Radiology

Volumn 22, Issue 9, 2012, Pages 1955-1962

  Abou Elkacem, Lotfi ^a   Björn, Saskia ^b   Doleschel, Dennis ^a   Ntziachristos, Vasilis ^b   Schulz, Ralf ^b   Hoffman, Robert M ^c   Kiessling, Fabian ^a   Lederle, Wiltrud ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c ANTICANCER INC   (United States)

Author keywords

Mesoscopic epi fluorescence tomography; Micro computed tomography; Red green fluorescent protein; Tumour volume; Ultrasound

Indexed keywords

GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CANCER CELL; COLON CANCER; COMPARATIVE STUDY; DIAGNOSTIC TEST ACCURACY STUDY; ECHOGRAPHY; EX VIVO STUDY; FEMALE; HUMAN; HUMAN CELL; ILLUMINATION REFLECTANCE IMAGING; IMAGING SYSTEM; IN VIVO STUDY; MESOSCOPIC EPIFLUORESCENCE TOMOGRAPHY; MICRO-COMPUTED TOMOGRAPHY; MOUSE; NON INVASIVE MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; TOMOGRAPHY; TUMOR VOLUME; TUMOR XENOGRAFT;

ANIMALS; CELL LINE, TUMOR; COLONIC NEOPLASMS; FEMALE; GREEN FLUORESCENT PROTEINS; MICE; MICE, NUDE; MOLECULAR IMAGING; NEOPLASM PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84864717575     PISSN: 09387994     EISSN: 14321084     Source Type: Journal    
DOI: 10.1007/s00330-012-2462-x     Document Type: Article

References (20)

1. Graham KC, Wirtzfeld LA, MacKenzie LT et al (2005) Threedimensional high-frequency ultrasound imaging for longitudinal evaluation of liver metastases in preclinical models. Cancer Res 65:5231-5237
2. Wessels JT, Busse AC, Mahrt J, Dullin C, Grabbe E, Mueller GA (2007) In vivo imaging in experimental preclinical tumor research-A review. Cytometry 71:542-549
3. Weissleder R, Pittet MJ (2008) Imaging in the era of molecular oncology. Nature 452:580-589
4. Hoffman RM (2005) The multiple uses of fluorescent proteins to visualize cancer in vivo. Nat Rev Cancer 5:796-806
5. Abou-Elkacem L, Gremse F, Barth S, Hoffman RM, Kiessling F, Lederle W (2011) Comparison of μCT, MRI and optical reflectance imaging for assessing the growth of GFP/RFP-expressing tumors. Anticancer Res 31:2907-2913
6. Ntziachristos V, Ripoll J, Wang LV, Weissleder R (2005) Looking and listening to light: The evolution of whole-body photonic imaging. Nat Biotech 23:313-320
7. Björn S, Ntziachristos V, Schulz R (2010) Mesoscopic epifluorescence tomography: Reconstruction of superficial and deep fluorescence in highly-scattering media. Opt Express 18:8422-8429
8. Björn S, Englmeier KH, Ntziachristos V, Schulz R (2011) Reconstruction of fluorescence distribution hidden in biological tissue using mesoscopic epifluorescence tomography. J Biomed Opt 16:046005
9. Jiang P, Yamauchi K, Yang M et al (2006) Tumor cells genetically labeled with GFP in the nucleus and RFP in the cytoplasm for imaging cellular dynamics. Cell Cycle 5:1198-1201
10. Yamamoto N, Jiang P, Yang M et al (2004) Cellular dynamics visualized in live cells in vitro and in vivo by differential dualcolor nuclear-cytoplasmic fluorescent-protein expression. Cancer Res 64:4251-4256
11. Hoffman RM, Yang M (2006) Subcellular imaging in the live mouse. Nat Protoc 1:775-782
12. Cheung AMY, Brown AS, Hastie LA et al (2005) Threedimensional ultrasound biomicroscopy for xenograft growth analysis. Ultrasound Med Biol 31:865-870
13. Sarantopoulos A, Themelis G, Ntziachristos V (2010) Imaging the bio-distribution of fluorescent probes using multispectral epiillumination cryoslicing imaging. Mol Imaging Biol 13:874-885
14. Ntziachristos V (2010) Going deeper than microscopy: The optical imaging frontier in biology. Nat Methods 7:603-614
15. Vinegoni C, Pitsouli C, Razansky D, Perrimon N, Ntziachristos V (2007) In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography. Nat Methods 5:45-47
16. Weissleder R, Ntziachristos V (2003) Shedding light onto live molecular targets. Nat Med 9:123-128
17. Crane LMA, Themelis G, Pleijhuis RG et al (2011) Intraoperative multispectral fluorescence imaging for the detection of the sentinel lymph node in cervical cancer: A novel concept. Mol Imaging Biol 13:1043-1049
18. van Dam GM, Themelis G, Crane LMA et al (2011) Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-A targeting: First in-human results. Nat Med 17:1315-1319
19. Katz MH, Takimoto S, Spivack D, Moossa AR, Hoffman RM, Bouvet M (2003) A novel red fluorescent protein orthotopic pancreatic cancer model for the preclinical evaluation of chemotherapeutics. J Surg Res 113:151-160
20. Deliolanis NC, Wurdinger T, Pike L et al (2011) In vivo tomographic imaging of red-shifted fluorescent proteins. Biomed Opt Express 2:887-900