Biophysical characterization of Met-G-CSF: Effects of different site-specific mono-pegylations on protein stability and aggregation

PLoS ONE

Volumn 7, Issue 8, 2012, Pages

  Natalello, Antonino ^a,b   Ami, Diletta ^a,b   Collini, Maddalena ^a   D'Alfonso, Laura ^a   Chirico, Giuseppe ^a   Tonon, Giancarlo ^c   Scaramuzza, Silvia ^d   Schrepfer, Rodolfo ^d   Doglia, Silvia Maria ^a,b  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b INFM   (Italy)

^c IRCCS MULTIMEDICA   (Italy)

^d Bio Ker srl c/o Sardinia Scientific and Technological Park   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMINE; GRANULOCYTE COLONY STIMULATING FACTOR; METHIONINE; METHIONYL GRANULOCYTE COLONY STIMULATING FACTOR; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; CIRCULAR DICHROISM; CONTROLLED STUDY; FLUORESCENCE SPECTROSCOPY; INFRARED SPECTROSCOPY; KINETICS; LIGHT SCATTERING; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PEGYLATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN STRUCTURE; THERMOSTABILITY;

BIOPHYSICS; CIRCULAR DICHROISM; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; LIGHT; METHIONINE; MODELS, STATISTICAL; PROTEIN CONFORMATION; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SPECTROMETRY, FLUORESCENCE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TEMPERATURE;

EID: 84864697208     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0042511     Document Type: Article

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