Mutations at the putative active cavity of styrene monooxygenase: Enhanced activity and reversed enantioselectivity

Journal of Biotechnology

Volumn 161, Issue 3, 2012, Pages 235-241

  Lin, Hui ^a,b   Tang, De Fang ^a   Ahmed, Abeer Ahmed Qaed ^a,b   Liu, Yan ^a   Wu, Zhong Liu ^a  

^a CHENGDU INSTITUTE OF BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Biocatalysis; Chiral epoxide; Protein engineering; Rational design; Styrene monooxygenase

Indexed keywords

BIOCATALYSIS; CHIRAL EPOXIDES; MONOOXYGENASES; PROTEIN ENGINEERING; RATIONAL DESIGN;

AMINO ACIDS; DEGRADATION; GENETIC ENGINEERING;

STYRENE;

1 PHENYLCYCLOHEXENE; BETA METHYL STYRENE; CYCLOHEXENE DERIVATIVE; HISTIDINE; OXYGENASE; PHENYL GROUP; SERINE; STYRENE; STYRENE OXYGENASE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BIOTRANSFORMATION; CATALYSIS; CRYSTAL STRUCTURE; ENANTIOSELECTIVITY; ENZYME ACTIVITY; EPOXIDATION; MOLECULAR DOCKING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS; SITE DIRECTED MUTAGENESIS; STEREOCHEMISTRY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BIOCATALYSIS; BIOTRANSFORMATION; CATALYTIC DOMAIN; EPOXY COMPOUNDS; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION; OXYGENASES; PSEUDOMONAS PUTIDA; SEQUENCE ALIGNMENT; STEREOISOMERISM; STYRENE; SUBSTRATE SPECIFICITY;

EID: 84864567619     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.06.028     Document Type: Article

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