Determination of the absolute number of transgene copies in CMVFut transgenic pigs;Określenie liczby kopii transgenu u transgenicznych świń CMVfut

Annals of Animal Science

Volumn 12, Issue 3, 2012, Pages 349-356

  Lipiński, Daniel ^a,b   Zeyland, Joanna ^a   Pławski, Andrzej ^b   Słomski, Ryszard ^a,b  

^a POZNA UNIVERSITY OF LIFE SCIENCES   (Poland)

^b INSTITUTE OF HUMAN GENETICS   (Poland)

Author keywords

Animal transgenesis; RT PCR; Transgene copy number

Indexed keywords

EID: 84864484211     PISSN: 16423402     EISSN: None     Source Type: Journal    
DOI: 10.2478/v10220-012-0029-z     Document Type: Article

References (16)

1. Ballester M., Castelló A., Ibáñez E., Sánchez A., Folch J.M. (2004). Real-time quantitative P CR-based system for determining transgene copy number in transgenic animals. Biotechniques, 37: 610-613.
2. Costa C., Zhao L,Burton W.V., Bondioli K.R., Williams B.L., Hoagland T.A., Ditullio P.A., Ebert KM., Fodor W.L. (1999). Expression of the human alpha 1,2-fucosyltransferase in transgenic pigs modifies the cell surface carbohydrate phenotype and confers resistance to human serum-mediated cytolysis. FASEB J., 13: 1762-1773.
3. Folger K.R., Thomas K., Capecchi M.R. (1985). Nonreciprocal exchanges of information between DNA duplexes coinjected into mammalian cell nuclei. Mol. Cell. Biol., 5: 59-69.
4. Folger K.R., Wong E.A., Wahl G., Capecchi M.R. (1982). Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules. Mol. Cell. Biol., 2: 372-1387.
5. Galili U., Shohet S.B., Kobrin E., Stults C.L., Macher B.A. (1988). Man, apes, and Old World monkeys differ from other mammals in the expression of alpha-galactosyl epitopes on nucleated cells. J. Biol. Chem., 263: 17755-17762.
6. Galili U., Swanson K. (1991). Gene sequences suggest inactivation of alpha-1,3-galactosyltransferase in catarrhines after the divergence of apes from monkeys. Proc. Nat. Acad. Sci. U. S. A., 88: 7401-7404.
7. Ingham D.J., Beer S., Money S., Hansen GH. (2001). Quantitative real-time PCR assay for determining transgene copy number in transformed plants. Biotechniques, 31: 132-140.
8. Jura J., Słomski R., Smorag Z., Gajda B., Wieczorek J., Lipiński D., Kalak R., Juzwa W., Zeyland J. (2004). Production of transgenic pigs suitable for xenotransplantation with the use of standard DNA microinjection. Ann. Anim. Sci., 4, 2: 321-327.
9. Keshet I., Lieman-Hurwitz J., Cedar H. (1986). DNA methylation affects the formation of active chromatin. Cell, 44: 535-543.
10. Lipiński D., Jura J., Zeyland J., Juzwa W., Mały E., Kalak R., Bochenek M., Pławski A., Szalata M., Smorag Z., Słomski R. (2010). Production of transgenic pigs expressing human α1,2-fucosyltransferase to avoid humoral xenograft rejection. Med. Wet., 66: 316-322.
11. Milot E., Strouboulis J., Trimborn T., Wijgerde M., de Boer E., Langeveld A., Tan-Un K., Vergeer W., Yannoutsos N., Grosveld F., Fraser P. (1996). Heterochromatin effects in the frequency and duration of LCR-mediated gene transcription. Cell, 87: 105-114.
12. Muller K., Heller H., Doerfler W. (2001). Foreign DNA integration. J. Biol. Chem., 276: 14271-14278.
13. Sandrin M.S., McKenzie I.F. (1994). Gal alpha (1,3)Gal, the major xenoantigen(s) recognised in pigs by human natural antibodies. Immunol. Rev., 141: 169-190.
14. Sharma A., Okabe J., Birch P., McClellan S.B., Martin M.J., Platt J.L., Logan J.S. (1996). Reduction in the le zvel of Gal(alpha1,3)Gal in transgenic mice and pigs by the expression of an alpha(1,2)fucosyltransferase. Proc. Nat. Acad. Sci. U. S. A., 93: 7190-7195.
15. Shitara H., Sato A., Hayashi J., Mizushima N., Yonekawa H., Taya C. (2004). Simple method of zygosity identification in transgenic mice by real-time quantitative PCR. Transgenic Res., 13: 191-194.
16. Tesson L., Heslan J.M., Ménoret S., Anegon I (2002). Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR. Transgenic Res., 11: 43-48.