Building droplet-based microfluidic systems for biological analysis

Biochemical Society Transactions

Volumn 40, Issue 4, 2012, Pages 615-623

  Niu, Xize ^a   DeMello, Andrew J ^b  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b ETH ZURICH   (Switzerland)

Author keywords

Droplet based microfluidics; Fluid dynamics; Segmented flow

Indexed keywords

OIL; SURFACTANT;

ANALYTICAL PARAMETERS; CONFERENCE PAPER; FLOW KINETICS; FLUID FLOW; MICROFLUIDICS; PARTICLE SIZE; PRIORITY JOURNAL; SYNTHESIS; VOLUMETRY;

MICROFLUIDICS;

EID: 84864383592     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST20120005     Document Type: Conference Paper

References (103)

1. Tawfik, D.S. and Griffiths, A.D. (1998) Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 16, 652-656 (Pubitemid 28334323)
2. Theberge, A.B., Courtois, F., Schaerli, Y., Fischlechner, M., Abell, C., Hollfelder, F. and Huck, W.T.S. (2010) Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew. Chem. Int. Ed. 49, 5846-5868
3. Huebner, A., Sharma, S., Srisa-Art, M., Hollfelder, F., Edel, J.B. and deMello, A.J. (2008) Microdroplets: a sea of applications? Lab Chip 8, 1244-1254
4. Solvas, X.C.I. (2011) Droplet microfluidics: recent developments and future applications. Chem. Commun. 47, 1936-1942
5. Song, H., Chen, D.L. and Ismagilov, R.F. (2006) Reactions in droplets in microflulidic channels. Angew. Chem. Int. Ed. 45, 7336-7356
6. deMello, A.J. (2006) Control and detection of chemical reactions in microfluidic systems. Nature 442, 394-402
7. Schaerli, Y., Wootton, R.C., Robinson, T., Stein, V., Dunsby, C., Neil, M.A.A., French, P.M.W., deMello, A.J., Abell, C. and Hollfelder, F. (2009) Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Anal. Chem. 81, 302-306
8. Song, H. and Ismagilov, R.F. (2003) Millisecond kinetics on a microfluidic chip using nanoliters of reagents. J. Am. Chem. Soc. 125, 14613-14619 (Pubitemid 37452397)
9. Huebner, A., Srisa-Art, M., Holt, D., Abell, C., Hollfelder, F., deMello, A.J. and Edel, J.B. (2007) Quantitative detection of protein expression in single cells using droplet microfluidics. Chem. Commun., 1218-1220 (Pubitemid 46412142)
10. Srisa-Art, M., deMello, A.J. and Edel, J.B. (2007) High-throughput DNA droplet assays using picoliter reactor volumes. Anal. Chem. 79, 6682-6689 (Pubitemid 47395055)
11. Clausell-Tormos, J., Lieber, D., Baret, J.C., El-Harrak, A., Miller, O.J., Frenz, L., Blouwolff, J., Humphry, K.J., Koster, S., Duan, H. et al. (2008) Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms. Chem. Biol. 15, 427-437 (Pubitemid 351645111)
12. Gulati, S., Rouilly, V., Niu, X.Z., Chappell, J., Kitney, R.I., Edel, J.B., Freemont, P.S. and deMello, A.J. (2009) Opportunities for microfluidic technologies in synthetic biology. J. R. Soc. Interface 6 Suppl. 4, S493-S506
13. Kintses, B., van Vliet, L.D., Devenish, S.R.A. and Hollfelder, F. (2010) Microfluidic droplets: new integrated workflows for biological experiments. Curr. Opin. Chem. Biol. 14, 548-555
14. Teh, S.Y., Lin, R., Hung, L.H. and Lee, A.P. (2008) Droplet microfluidics. Lab Chip 8, 198-220 (Pubitemid 351191417)
15. Baroud, C.N., Gallaire, F. and Dangla, R. (2010) Dynamics of microfluidic droplets. Lab Chip 10, 2032-2045
16. Shah, R.K., Shum, H.C., Rowat, A.C., Lee, D., Agresti, J.J., Utada, A.S., Chu, L.Y., Kim, J.W., Fernandez-Nieves, A., Martinez, C.J. and Weitz, D.A. (2008) Designer emulsions using microfluidics. Mater. Today 11, 18-27 (Pubitemid 351381609)
17. Xia, Y.N. and Whitesides, G.M. (1998) Soft lithography. Annu. Rev. Mater. Sci. 28, 153-184
18. Linder, V., Sia, S.K. and Whitesides, G.M. (2005) Reagent-loaded cartridges for valveless and automated fluid delivery in microfluidic devices. Anal. Chem. 77, 64-71 (Pubitemid 40096669)
19. Chabert, M., Dorfman, K.D., de Cremoux, P., Roeraade, J. and Viovy, J.L. (2006) Automated microdroplet platform for sample manipulation and polymerase chain reaction. Anal. Chem. 78, 7722-7728 (Pubitemid 44788715)
20. Nightingale, A.M., Krishnadasan, S.H., Berhanu, D., Niu, X., Drury, C., McIntyre, R., Valsami-Jones, E. and deMello, J.C. (2011) A stable droplet reactor for high temperature nanocrystal synthesis. Lab Chip 11, 1221-1227
21. Mazutis, L., Baret, J.C., Treacy, P., Skhiri, Y., Araghi, A.F., Ryckelynck, M., Taly, V. and Griffiths, A.D. (2009) Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme. Lab Chip 9, 2902-2908
22. Pan, X.Y., Zeng, S.J., Zhang, Q.Q., Lin, B.C. and Qin, J.H. (2011) Sequential microfluidic droplet processing for rapid DNA extraction. Electrophoresis 32, 3399-3405
23. Prakash, M. and Gershenfeld, N. (2007) Microfluidic bubble logic. Science 315, 832-835
24. Fuerstman, M.J., Garstecki, P. and Whitesides, G.M. (2007) Coding/decoding and reversibility of droplet trains in microfluidic networks. Science 315, 828-832
25. Sessoms, D.A., Amon, A., Courbin, L. and Panizza, P. (2010) Complex dynamics of droplet traffic in a bifurcating microfluidic channel: periodicity, multistability, and selection rules. Phys. Rev. Lett. 105, 154501
26. Bringer, M.R., Gerdts, C.J., Song, H., Tice, J.D. and Ismagilov, R.F. (2004) Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets. Philos. Trans. R. Soc. London Ser. A 362, 1087-1104 (Pubitemid 38652649)
27. Liau, A., Karnik, R., Majumdar, A. and Cate, J.H. D. (2005) Mixing crowded biological solutions in milliseconds. Anal. Chem. 77, 7618-7625 (Pubitemid 41746229)
28. Chabert, M. and Viovy, J.L. (2008) Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells. Proc. Natl. Acad. Sci. U.S.A. 105, 3191-3196 (Pubitemid 351723530)
29. Tan, Y.C., Fisher, J.S., Lee, A.I., Cristini, V. and Lee, A.P. (2004) Design of microfluidic channel geometries for the control of droplet volume, chemical concentration, and sorting. Lab Chip 4, 292-298 (Pubitemid 39150228)
30. Courtois, F., Olguin, L.F., Whyte, G., Bratton, D., Huck, W.T.S., Abell, C. and Hollfelder, F. (2008) An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. ChemBioChem 9, 439-446
31. Mary, P., Abate, A.R., Agresti, J.J. and Weitz, D.A. (2011) Controlling droplet incubation using close-packed plug flow. Biomicrofluidics 5, 24101
32. Ahn, K., Agresti, J., Chong, H., Marquez, M. and Weitz, D.A. (2006) Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels. Appl. Phys. Lett. 88, 264105
33. Niu, X., Gulati, S., Edel, J.B. and deMello, A.J. (2008) Pillar-induced droplet merging in microfluidic circuits. Lab Chip 8, 1837-1841
34. Bremond, N., Thiam, A.R. and Bibette, J. (2008) Decompressing emulsion droplets favors coalescence. Phys. Rev. Lett. 100, 024501
35. Priest, C., Herminghaus, S. and Seemann, R. (2006) Controlled electrocoalescence in microfluidics: targeting a single lamella. Appl. Phys. Lett. 89, 134101
36. Zagnoni, M. and Cooper, J.M. (2010) A microdroplet-based shift register. Lab Chip 10, 3069-3073
37. Song, H., Li, H.W., Munson, M.S., Van Ha, T.G. and Ismagilov, R.F. (2006) On-chip titration of an anticoagulant argatroban and determination of the clotting time within whole blood or plasma using a plug-based microfluidic system. Anal. Chem. 78, 4839-4849 (Pubitemid 44100616)
38. Li, L., Boedicker, J.Q. and Ismagilov, R.F. (2007) Using a multijunction microfluidic device to inject substrate into an array of preformed plugs without cross-contamination: comparing theory and experiments. Anal. Chem. 79, 2756-2761 (Pubitemid 46595628)
39. Evans, H.M., Surenjav, E., Priest, C., Herminghaus, S., Seemann, R. and Pfohl, T. (2009) In situ formation, manipulation, and imaging of droplet-encapsulated fibrin networks. Lab Chip 9, 1933-1941
40. Wang, M., Roman, G.T., Schultz, K., Jennings, C. and Kennedy, R.T. (2008) Improved temporal resolution for in vivo microdialysis by using segmented flow. Anal. Chem. 80, 5607-5615
41. van Dijke, K., Kobayashi, I., Schroen, K., Uemura, K., Nakajima, M. and Boom, R. (2010) Effect of viscosities of dispersed and continuous phases in microchannel oil-in-water emulsification. Microfluid. Nanofluid. 9, 77-85
42. Baret, J.C. (2012) Surfactants in droplet-based microfluidics. Lab Chip 12, 422-433
43. Thorsen, T., Roberts, R.W., Arnold, F.H. and Quake, S.R. (2001) Dynamic pattern formation in a vesicle-generating microfluidic device. Phys. Rev. Lett. 86, 4163-4166 (Pubitemid 32443957)
44. Dittrich, P.S., Jahnz, M. and Schwille, P. (2005) A new embedded process for compartmentalized cell-free protein expression and on-line detection in microfluidic devices. ChemBioChem 6, 811-814 (Pubitemid 40873736)
45. Niu, X.Z., Zhang, M.Y., Peng, S.L., Wen, W.J. and Sheng, P. (2007) Real-time detection, control, and sorting of microfluidic droplets. Biomicrofluidics 1, 44101
46. Roach, L.S., Song, H. and Ismagilov, R.F. (2005) Controlling nonspecific protein adsorption in a plug-based microfluidic system by controlling interfacial chemistry using fluorous-phase surfactants. Anal. Chem. 77, 785-796 (Pubitemid 40194142)
47. Baret, J.C., Beck, Y., Billas-Massobrio, I., Moras, D. and Griffiths, A.D. (2010) Quantitative cell-based reporter gene assays using droplet-based microfluidics. Chem. Biol. 17, 528-536
48. Theberge, A.B., Mayot, E., El Harrak, A., Kleinschmidt, F., Huck, W.T. S. and Griffiths, A.D. (2012) Microfluidic platform for combinatorial synthesis in picolitre droplets. Lab Chip 12, 1320-1326
49. Bagwe, R.P., Kanicky, J.R., Palla, B.J., Patanjali, P.K. and Shah, D.O. (2001) Improved drug delivery using microemulsions: rationale, recent progress, and new horizons. Crit. Rev. Ther. Drug Carrier Syst. 18, 77-140 (Pubitemid 32319398)
50. Bibette, J., Leal Calderon, F. and Poulin, P. (1999) Emulsions: basic principles. Rep. Prog. Phys. 62, 969-1033
51. Bibette, J., Morse, D.C., Witten, T.A. and Weitz, D.A. (1992) Stability criteria for emulsions. Phys. Rev. Lett. 69, 2439-2442
52. Holtze, C., Rowat, A.C., Agresti, J.J., Hutchison, J.B., Angile, F.E., Schmitz, C.H.J., Koster, S., Duan, H., Humphry, K.J., Scanga, R.A. et al. (2008) Biocompatible surfactants for water-in-fluorocarbon emulsions. Lab Chip 8, 1632-1639
53. West, J., Becker, M., Tombrink, S. and Manz, A. (2008) Micro total analysis systems: latest achievements. Anal. Chem. 80, 4403-4419 (Pubitemid 351842328)
54. Kawakatsu, T., Tragardh, G., Tragardh, C., Nakajima, M., Oda, N. and Yonemoto, T. (2001) The effect of the hydrophobicity of microchannels and components in water and oil phases on droplet formation in microchannel water-in-oil emulsification. Colloids Surf. A 179, 29-37 (Pubitemid 32098219)
55. Brouzes, E., Medkova, M., Savenelli, N., Marran, D., Twardowski, M., Hutchison, J.B., Rothberg, J.M., Link, D.R., Perrimon, N. and Samuels, M.L. (2009) Droplet microfluidic technology for single-cell high-throughput screening. Proc. Natl. Acad. Sci. U.S.A. 106, 14195-14200
56. Kuhn, S., Hartman, R.L., Sultana, M., Nagy, K.D., Marre, S. and Jensen, K.F. (2011) Teflon-coated silicon microreactors: impact on segmented liquid-liquid multiphase flows. Langmuir 27, 6519-6527
57. Begolo, S., Colas, G., Viovy, J.L. and Malaquin, L. (2011) New family of fluorinated polymer chips for droplet and organic solvent microfluidics. Lab Chip 11, 508-512
58. Walser, M., Pellaux, R., Meyer, A., Bechtold, M., Vanderschuren, H., Reinhardt, R., Magyar, J., Panke, S. and Held, M. (2009) Novel method for high-throughput colony PCR screening in nanoliter-reactors. Nucleic Acids Res. 37, e57
59. Leng, X.F., Zhang, W.H., Wang, C.M., Cui, L.A. and Yang, C.J. (2010) Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR. Lab Chip 10, 2841-2843
60. Labrot, V., Schindler, M., Guillot, P., Colin, A. and Joanicot, M. (2009) Extracting the hydrodynamic resistance of droplets from their behavior in microchannel networks. Biomicrofluidics 3, 12804
61. Fuerstman, M.J., Lai, A., Thurlow, M.E., Shevkoplyas, S.S., Stone, H.A. and Whitesides, G.M. (2007) The pressure drop along rectangular microchannels containing bubbles. Lab Chip 7, 1479-1489 (Pubitemid 350013182)
62. Schindler, M. and Ajdari, A. (2008) Droplet traffic in microfluidic networks: a simple model for understanding and designing. Phys. Rev. Lett. 100, 044501
63. Anna, S.L., Bontoux, N. and Stone, H.A. (2003) Formation of dispersions using "flow focusing" in microchannels. Appl. Phys. Lett. 82, 364-366
64. Yobas, L., Martens, S., Ong, W.L. and Ranganathan, N. (2006) High-performance flow-focusing geometry for spontaneous generation of monodispersed droplets. Lab Chip 6, 1073-1079 (Pubitemid 44141686)
65. Kelly, R.T., Page, J.S., Marginean, I., Tang, K.Q. and Smith, R.D. (2009) Dilution-free analysis from picoliter droplets by nano-electrospray ionization mass spectrometry. Angew. Chem. Int. Ed. 48, 6832-6835
66. Link, D.R., Grasland-Mongrain, E., Duri, A., Sarrazin, F., Cheng, Z.D., Cristobal, G., Marquez, M. and Weitz, D.A. (2006) Electric control of droplets in microfluidic devices. Angew. Chem. Int. Ed. 45, 2556-2560 (Pubitemid 44105758)
67. Willaime, H., Barbier, V., Kloul, L., Maine, S. and Tabeling, P. (2006) Arnold tongues in a microfluidic drop emitter. Phys. Rev. Lett. 96, 054501
68. Baroud, C.N., de Saint Vincent, M.R. and Delville, J.P. (2007) An optical toolbox for total control of droplet microfluidics. Lab Chip 7, 1029-1033 (Pubitemid 47101741)
69. Nguyen, N.T., Ting, T.H., Yap, Y.F., Wong, T.N., Chai, J.C.K., Ong, W.L., Zhou, J., Tan, S.H. and Yobas, L. (2007) Thermally mediated droplet formation in microchannels. Appl. Phys. Lett. 91, 084102
70. Du, W.B., Li, L., Nichols, K.P. and Ismagilov, R.F. (2009) SlipChip. Lab Chip 9, 2286-2292
71. Shen, F., Du, W.B., Kreutz, J.E., Fok, A. and Ismagilov, R.F. (2010) Digital PCR on a SlipChip. Lab Chip 10, 2666-2672
72. Niu, X.Z., Zhang, B., Marszalek, R.T., Ces, O., Edel, J.B., Klug, D.R. and deMello, A.J. (2009) Droplet-based compartmentalization of chemically separated components in two-dimensional separations. Chem. Commun., 6159-6161
73. Edgar, J.S., Milne, G., Zhao, Y.Q., Pabbati, C.P., Lim, D.S.W. and Chiu, D.T. (2009) Compartmentalization of chemically separated components into droplets. Angew. Chem. Int. Ed. 48, 2719-2722
74. Roman, G.T., Wang, M., Shultz, K.N., Jennings, C. and Kennedy, R.T. (2008) Sampling and electrophoretic analysis of segmented flow streams using virtual walls in a microfluidic device. Anal. Chem. 80, 8231-8238
75. Shestopalov, I., Tice, J.D. and Ismagilov, R.F. (2004) Multi-step synthesis of nanoparticles performed on millisecond time scale in a microfluidic droplet-based system. Lab Chip 4, 316-321 (Pubitemid 39150231)
76. Niu, X., Gielen, F., Edel, J.B. and deMello, A.J. (2009) Electro-coalescence of digitally controlled droplets. Anal. Chem. 81, 7321-7325
77. Link, D.R., Anna, S.L., Weitz, D.A. and Stone, H.A. (2004) Geometrically mediated breakup of drops in microfluidic devices. Phys. Rev. Lett. 92, 054503
78. Hatch, A.C., Fisher, J.S., Tovar, A.R., Hsieh, A.T., Lin, R., Pentoney, S.L., Yang, D.L. and Lee, A.P. (2011) 1-Million droplet array with wide-field fluorescence imaging for digital PCR. Lab Chip 11, 3838-3845
79. Niu, X.Z., Gielen, F., Edel, J.B. and deMello, A.J. (2011) A microdroplet dilutor for high-throughput screening. Nat. Chem. 3, 437-442
80. Chen, D.L.L., Li, L., Reyes, S., Adamson, D.N. and Ismagilov, R.F. (2007) Using three-phase flow of immiscible liquids to prevent coalescence of droplets in microfluidic channels: criteria to identify the third liquid and validation with protein crystallization. Langmuir 23, 2255-2260 (Pubitemid 46406190)
81. Bai, Y.P., He, X.M., Liu, D.S., Patil, S.N., Bratton, D., Huebner, A., Hollfelder, F., Abell, C. and Huck, W.T.S. (2010) A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab Chip 10, 1281-1285
82. Baret, J.C., Miller, O.J., Taly, V., Ryckelynck, M., El-Harrak, A., Frenz, L., Rick, C., Samuels, M.L., Hutchison, J.B., Agresti, J.J. et al. (2009) Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip 9, 1850-1858
83. Abate, A.R., Agresti, J.J. and Weitz, D.A. (2010) Microfluidic sorting with high-speed single-layer membrane valves. Appl. Phys. Lett. 96, 203509
84. Wang, Z.C. and Zhe, J.A. (2011) Recent advances in particle and droplet manipulation for lab-on-a-chip devices based on surface acoustic waves. Lab Chip 11, 1280-1285
85. Baroud, C.N., Delville, J.P., Gallaire, F. and Wunenburger, R. (2007) Thermocapillary valve for droplet production and sorting. Phys. Rev. E: Stat., Nonlinear, Soft Matter Phys 75, 046302
86. Kiss, M.M., Ortoleva-Donnelly, L., Beer, N.R., Warner, J., Bailey, C.G., Colston, B.W., Rothberg, J.M., Link, D.R. and Leamon, J.H. (2008) High-throughput quantitative polymerase chain reaction in picoliter droplets. Anal. Chem. 80, 8975-8981
87. Beer, N.R., Hindson, B.J., Wheeler, E.K., Hall, S.B., Rose, K.A., Kennedy, I.M. and Colston, B.W. (2007) On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets. Anal. Chem. 79, 8471-8475 (Pubitemid 350171354)
88. Williams, R., Peisajovich, S.G., Miller, O.J., Magdassi, S., Tawfik, D.S. and Griffiths, A.D. (2006) Amplification of complex gene libraries by emulsion PCR. Nat. Methods 3, 545-550 (Pubitemid 43941773)
89. Zeng, Y., Novak, R., Shuga, J., Smith, M.T. and Mathies, R.A. (2010) High-performance single cell genetic analysis using microfluidic emulsion generator arrays. Anal. Chem. 82, 3183-3190
90. Agresti, J.J., Antipov, E., Abate, A.R., Ahn, K., Rowat, A.C., Baret, J.C., Marquez, M., Klibanov, A.M., Griffiths, A.D. and Weitz, D.A. (2010) Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc. Natl. Acad. Sci. U.S.A. 107, 4004-4009
91. Li, N., Gao, Y.A., Zheng, L.Q., Zhang, J., Yu, L. and Li, X.W. (2007) Studies on the micropolarities of bmimBF4/TX-100/toluene ionic liquid microemulsions and their behaviors characterized by UV-visible spectroscopy. Langmuir 23, 1091-1097
92. Grad, M., Tsai, C.C., Yu, M., Kwong, D.L., Wong, C.W. and Attinger, D. (2010) Transient sensing of liquid films in microfluidic channels with optofluidic microresonators. Meas. Sci. Technol. 21, 075204
93. Chan, K.L. A., Niu, X., deMello, A.J. and Kazarian, S.G. (2011) Generation of chemical movies: FT-IR spectroscopic imaging of segmented flows. Anal. Chem. 83, 3606-3609
94. Cecchini, M.P., Hong, J., Lim, C., Choo, J., Albrecht, T., deMello, A.J. and Edel, J.B. (2011) Ultrafast surface enhanced resonance Raman scattering detection in droplet-based microfluidic systems. Anal. Chem. 83, 3076-3081
95. Lin, Y.Q., Schiavo, S., Orjala, J., Vouros, P. and Kautz, R. (2008) Microscale LC-MS-NMR platform applied to the identification of active cyanobacterial metabolites. Anal. Chem. 80, 8045-8054
96. Fidalgo, L.M., Whyte, G., Ruotolo, B.T., Benesch, J.L. P., Stengel, F., Abell, C., Robinson, C.V. and Huck, W.T.S. (2009) Coupling microdroplet microreactors with mass spectrometry: reading the contents of single droplets online. Angew. Chem. Int. Ed. 48, 3665-3668
97. Hatakeyama, T., Chen, D.L. and Ismagilov, R.F. (2006) Microgram-scale testing of reaction conditions in solution using nanoliter plugs in microfluidics with detection by MALDI-MS. J. Am. Chem. Soc. 128, 2518-2519 (Pubitemid 43327912)
98. Srisa-Art, M., deMello, A.J. and Edel, J.B. (2010) High-efficiency single-molecule detection within trapped aqueous microdroplets. J. Phys. Chem. B 114, 15766-15772
99. Solvas, X.C.I., Srisa-Art, M., deMello, A.J. and Edel, J.B. (2010) Mapping of fluidic mixing in microdroplets with 1 μs time resolution using fluorescence lifetime imaging. Anal. Chem. 82, 3950-3956
100. Wang, G., Lim, C., Chen, L., Chon, H., Choo, J., Hong, J. and deMello, A.J. (2009) Surface-enhanced Raman scattering in nanoliter droplets: towards high-sensitivity detection of mercury (II) ions. Anal. Bioanal. Chem. 394, 1827-1832
101. Matzneller, P. and Brunner, M. (2011) Recent advances in clinical microdialysis. Trends Anal. Chem. 30, 1497-1504
102. Rogers, M., Leong, C., Niu, X.Z., de Mello, A., Parker, K.H. and Boutelle, M.G. (2011) Optimisation of a microfluidic analysis chamber for the placement of microelectrodes. Phys. Chem. Chem. Phys. 13, 5298-5303
103. Wang, M., Slaney, T., Mabrouk, O. and Kennedy, R.T. (2010) Collection of nanoliter microdialysate fractions in plugs for off-line in vivo chemical monitoring with up to 2 s temporal resolution. J. Neurosci. Methods 190, 39-48