Assessment of Candida shehatae viability by flow cytometry and fluorescent probes

Journal of Microbiological Methods

Volumn 91, Issue 1, 2012, Pages 8-13

  Monthéard, Julie ^a,b,c   Garcier, Sandy ^a,b,c   Lombard, Eric ^a,b,c   Cameleyre, Xavier ^a,b,c   Guillouet, Stéphane ^a,b,c   Molina Jouve, Carole ^a,b,c   Alfenore, Sandrine ^a,b,c  

^a UNIVERSITÉ DE TOULOUSE   (France)

^b UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

^c CNRS   (France)

Author keywords

Activity; Candida shehatae; Flow cytometry; Fluorescence

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE ACETOXYMETHYL ESTER; FLUORESCENT DYE; POLOXAMER; PROPIDIUM IODIDE; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BIOREACTOR; CANDIDA; CANDIDA SHEHATAE; CELL VIABILITY; CONTROLLED STUDY; FERMENTATION; FLOW CYTOMETRY; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REPRODUCIBILITY; TEMPERATURE;

CANDIDA; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; MICROBIAL VIABILITY; PROPIDIUM; REPRODUCIBILITY OF RESULTS; STAINING AND LABELING;

CANDIDA SHEHATAE;

EID: 84864354058     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.07.002     Document Type: Article

References (28)

1. Alfenore S., Molina-Jouve C., Guillouet S.E., Uribelarrea J.L., Goma G., Benbadis L. Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process. Appl. Microbiol. Biotechnol. 2002, 60:67.
2. Attfield P.V., Kletsas S., Veal D.A., van Rooijen R., Bell P.J.L. Use of flow cytometry to monitor cell damage and predict fermentation activity of dried yeasts. J. Appl. Microbiol. 2000, 89:207-214.
3. Beney L., Gervais P. Influence of the fluidity of the membrane on the response of microorganisms to environmental stresses. Appl. Microbiol. Biotechnol. 2001, 57:34-42.
4. Bouchez J.C., Cornu M., Danzart M., Leveau J.Y., Duchiron F., Bouix M. Physiological significance of the cytometric distribution of fluorescent yeasts after viability staining. Biotechnol. Bioeng. 2004, 86:520-530.
5. Bouix M., Leveau J.Y. Rapid assessment of yeast viability and yeast vitality during alcoholic fermentation. J. Inst. Brew. 2001, 107:217-225.
6. Boyd A.R., Gunasekera T.S., Attfield P.V., Simic K., Vincent S.F., Veal D.A. A flow-cytometric method for determination of yeast viability and cell number in a brewery. FEMS Yeast Res. 2003, 3:11-15.
7. Breeuwer P., Drocourt J.L., Rombouts F.M., Abee T. Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry. Appl. Environ. Microbiol. 1994, 60:1467-1472.
8. Davey H.M., Kell D.B. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol. Rev. 1996, 60:641-696.
9. Deere D., Shen J., Vesey G., Bell P., Bissinger P., Veal D. Flow cytometry and cell sorting for yeast viability assessment and cell selection. Yeast 1998, 14:147-160.
10. Doherty S.B., Wang L., Ross R.P., Stanton C., Fitzgerald G.F., Brodkorb A. Use of viability staining in combination with flow cytometry for rapid viability assessment of Lactobacillus rhamnosus GG in complex protein matrices. J. Microbiol. Methods 2010, 82:301-310.
11. Fromanger R., Guillouet S.E., Uribelarrea J.L., Molina-Jouve C., Cameleyre X. Effect of controlled oxygen limitation on Candida shehatae physiology for ethanol production from xylose and glucose. J. Ind. Microbiol. Biotechnol. 2010, 37:437-445.
12. Gnansounou E. Production and use of lignocellulosic bioethanol in Europe: current situation and perspectives. Bioresour. Technol. 2010, 101:4842-4850.
13. Hernlem B., Hua S.-S. Dual fluorochrome flow cytometric assessment of yeast viability. Curr. Microbiol. 2010, 61:57-63.
14. Hohenblum H., Borth N., Mattanovich D. Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry. J. Biotechnol. 2003, 102:281-290.
15. Jones K.H., Senft J.A. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate and propidium iodide. J. Histochem. Cytochem. 1985, 33:77-79.
16. Kastner J.R., Jones W.J., Roberts R.S. Oxygen starvation induces cell death in Candida shehatae fermentations of d-xylose, but not d-glucose. Appl. Microbiol. Biotechnol. 1999, 51:780-785.
17. Malacrinò P., Zapparoli G., Torriani S., Dellaglio F. Rapid detection of viable yeasts and bacteria in wine by flow cytometry. J. Microbiol. Methods 2001, 45:127-134.
18. Papadimitriou K., Pratsinis H., Nebe-von-Caron G., Kletsas D., Tsakalidou E. Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes. Int. J. Food Microbiol. 2006, 111:197-205.
19. Parisi-Duchêne E., Reibel C., Grawey I., Heller R., Mazurier I., de Briel D.A., Moskovtchenko P. Rapid antifungal susceptibility testing of fluconazole and amphotericin B by flow cytometry using FUN-1: a preliminary study. J.Med.Mycol. 2006, 16:126-133.
20. Postgate J.R., Crumpton J.E., Hunter J.R. The measurement of bacterial viabilities by slide culture. J. Gen. Microbiol. 1961, 24:15-24.
21. Ramani R., Ramani A., Wong S.J. Rapid flow cytometric susceptibility testing of Candida albicans. J. Clin. Microbiol. 1997, 35:2320-2324.
22. Sanchez-Gonzalez Y., Cameleyre X., Molina-Jouve C., Goma G., Alfenore S. Dynamic microbial response under ethanol stress to monitor Saccharomyces cerevisiae activity in different initial physiological states. Bioprocess Biosyst. Eng. 2009, 32:459-467.
23. Slininger P.J., Bothast R.J., Okos M.R., Ladisch M.R. Comparative evaluation of ethanol production by xylose fermenting yeasts presented high xylose concentrations. Biotechnol. Lett. 1985, 7:431-436.
24. Toivola A., Yarrow D., Van Den Bosch E., Van Dijken J.P., Scheffers W.A. Alcoholic fermentation of d-xylose by yeasts. Appl. Environ. Microbiol. 1984, 47:1221-1223.
25. Vale-Silva L.A., Buchta V., Vokurková D., Pour M. Investigation of the mechanism of action of 3-(4-bromophenyl)-5-acyloxymethyl-2,5-dihydrofuran-2-one against Candida albicans by flow cytometry. Bioorg. Med. Chem. Lett. 2006, 16:2492-2495.
26. Van Zandycke S.M., Simal O., Gualdoni S., Smart K.A. Determination of yeast viability using fluorophores. J. Am. Soc. Brew. Chem. 2003, 61:15-22.
27. Veal D.A., Deere D., Ferrari B., Piper J., Attfield P.V. Fluorescence staining and flow cytometry for monitoring microbial cells. J. Immunol. Methods 2000, 243:191-210.
28. Villarino A., Bouvet O.M.M., Regnault B., Martin-Delautre S., Grimont P.A.D. Exploring the frontier between life and death in Escherichia coli: evaluation of different viability markers in live and heat- or UV-killed cells. Res. Microbiol. 2000, 151:755-768.