Mass spectrometry-based sequencing of protein C-terminal peptide using α-carboxyl group-specific derivatization and COOH capturing

Analytical Biochemistry

Volumn 428, Issue 2, 2012, Pages 167-172

  Nakajima, Chihiro ^a   Kuyama, Hiroki ^a   Tanaka, Koichi ^a  

^a SHIMADZU CORPORATION   (Japan)

Author keywords

C terminal sequencing; COOH capture; COOH specific derivatization

Indexed keywords

AMINES; DESORPTION; MASS SPECTROMETRY;

C-TERMINAL PEPTIDES; COOH CAPTURE; DERIVATIZATIONS; ENZYMATIC PROTEOLYSIS; MATRIX ASSISTED LASER DESORPTION/IONIZATION; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY; TERMINAL SEQUENCING;

PEPTIDES;

3 AMINOPROPYLTRIS (2,4,6 TRIMETHOXYPHENYL)PHOSPHONIUM BROMIDE; ALPHA CARBONYL; AMINE; BROMINE DERIVATIVE; CALMODULIN; CARBONYL DERIVATIVE; CYTOCHROME C; GALANIN; OXAZOLONE; PROPYLAMIDATION; PYROGLUTAMIC ACID; SUPEROXIDE DISMUTASE; TRIOSEPHOSPHATE ISOMERASE; UNCLASSIFIED DRUG;

AMIDATION; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; FRAGMENTATION REACTION; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DEGRADATION; TANDEM MASS SPECTROMETRY;

EID: 84864332072     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.06.016     Document Type: Article

References (15)

1. W.J. Henzel, T.M. Billeci, J.T. Stults, S.C. Wong, C. Gremley, and C. Watanabe Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases Proc. Natl. Acad. Sci. USA 90 1993 5011 5015 (Pubitemid 23182965)
2. J.J. Chung, S. Shikano, Y. Hanyu, and M. Li Functional diversity of protein C-termini: more than zipcoding? Trends Cell Biol. 12 2002 146 150 (Pubitemid 34164654)
3. J.J. Chung, H. Yang, and M. Li Genome-wide analyses of carboxyl-terminal sequences Mol. Cell. Proteomics 2 2003 173 181
4. F.X. Gomis-Ruth Structure and mechanism of metallocarboxypeptidases Crit. Rev. Biochem. Mol. Biol. 43 2008 319 345
5. J.H. Cox, R.A. Dean, C.R. Roberts, and C.M. Overall Matrix metalloproteinase processing of CXCL11/I-TAC results in loss of chemoattractant activity and altered glycosaminoglycan binding J. Biol. Chem. 283 2008 19389 19399
6. F. Impens, N. Colaert, K. Helsens, K. Plasman, P. Van Damme, J. Vandekerckhove, and K. Gevaert MS-driven protease substrate degradomics Proteomics 10 2010 1284 1296
7. H. Kuyama, K. Shima, K. Sonomura, M. Yamaguchi, E. Ando, O. Nishimura, and S. Tsunasawa A simple and successful C-terminal sequencing analysis of proteins by mass spectrometry Proteomics 8 2008 1539 1550 (Pubitemid 351580201)
8. C. Nakajima, H. Kuyama, T. Nakazawa, and O. Nishimura C-terminal sequencing of protein by MALDI mass spectrometry through the specific derivatization of the α-carboxyl group with 3-aminopropyltris(2,4,6- trimethoxyphenyl)phosphonium bromide Anal. Bioanal. Chem. 404 2012 125 132
9. C. Nakajima, H. Kuyama, T. Nakazawa, O. Nishimura, and S. Tsunasawa A method for N-terminal de novo sequencing of N-blocked proteins by mass spectrometry Analyst 136 2011 113 119
10. Z.-H. Huang, J. Wu, K.D.W. Roth, and Y. Yang A picomole-scale method for charge derivatization of peptides for sequence analysis by mass spectrometry Anal. Chem. 69 1997 137 144 (Pubitemid 127698230)
11. Z.-H. Huang, T. Shen, J. Wu, D.A. Gage, and J.T. Watson Protein sequencing by matrix-assisted laser desorption ionization-postsource decay-mass spectrometry analysis of the N-tris(2,4,6-trimethoxyphenyl)phosphine-acetylated tryptic digests Anal. Biochem. 268 1999 305 317 (Pubitemid 29147146)
12. H. Kuyama, K. Sonomura, and O. Nishimura Sensitive detection of phosphopeptides by matrix-assisted laser desorption/ionization mass spectrometry: use of alkylphosphonic acids as matrix additives Rapid Commun. Mass Spectrom. 22 2008 1109 1116 (Pubitemid 351600758)
13. H. Kuyama, C. Nakajima, T. Nakazawa, O. Nishimura, and S. Tsunasawa A new approach for detecting C-terminal amidation of proteins and peptides by mass spectrometry in conjunction with chemical derivatization Proteomics 9 2009 4063 4070
14. M. Yamaguchi, M. Oka, K. Nishida, M. Ishida, A. Hamazaki, H. Kuyama, E. Ando, T.-A. Okamura, N. Ueyama, S. Norioka, O. Nishimura, S. Tsunasawa, and T. Nakazawa Enhancement of MALDI-MS spectra of C-terminal peptides by the modification of proteins via an active ester generated in situ from an oxazolone Anal. Chem. 78 2006 7861 7869 (Pubitemid 44788732)
15. B. Keil Specificity of Proteolysis 1992 Springer-Verlag Berlin